LOCUS       sequence-46833-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate C R2-R1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..190
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(231..536)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(333..352)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agggccggat ctgcaggtcg accagctggc caagcttctt ctagaggtac cgcatgcgat
       61 atcgaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata
      121 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      181 agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      241 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      301 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      361 tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt
      421 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
      481 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
      541 ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
      601 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
      661 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
      721 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac
      781 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
      841 actgttatct ggctttt
//
