LOCUS       sequence-468330-        2037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2037)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV30
FEATURES             Location/Qualifiers
     source          1..2037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(367..383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(367..383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      422..453
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        454..556
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             557..1216
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(623..642)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1240..1267
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1279..1866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1359..1378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1888..1917)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1990..2007
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1991..2007
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgctctgctc tctccttgcc cttctcctgg gcactttctt tggggtcaga
       61 tctcagacta ttcatcaatg gccagcgacc ctggtgcagc ctgtgggcag cccgctctct
      121 ctggagtgca ctgtggaggg aacatcaaac cccaacctat actggtaccg acaggctgca
      181 ggcaggggcc tccagctgct cttctactcc attggtattg gccagatcag ctctgaggtg
      241 ccccagaatc tctcagcctc cagaccccag gaccggcagt tcatcctgag ttctaagaag
      301 ctcctcctca gtgactctgg cttctatctc tgtgtgagac gcagggaaga tggccgcgaa
      361 gaaaacgtca tagctgtttc ctgactgacc atttaaatca tacctgacct ccatagcaga
      421 aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt
      481 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      541 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      601 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      661 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      721 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
      781 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
      841 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
      901 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      961 taaagggttt attgagaata tgttttttgt ctcagccaat ccctgggtga gtttcaccag
     1021 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1081 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1141 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1201 gcagggcggg gcgtaatgag ggcccaaatg taatcacctg gctcaccttc gggtgggcct
     1261 ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1321 atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1681 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1741 tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1861 tctcaagaag atcctttgat tttctaccga agaaaggccc acccgtgaag gtgagccagt
     1921 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatcaagctt
     1981 gaattcgttt gtaaaacgac ggccagtgct aaccgtatcg cattggccat acctaca
//
