LOCUS       sequence-468332-        2052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2052)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV4-3
FEATURES             Location/Qualifiers
     source          1..2052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(382..398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(382..398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      437..468
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        469..571
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             572..1231
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(638..657)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1255..1282
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1294..1881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1374..1393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1903..1932)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2005..2022
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2006..2022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgggctgcag gctgctctgc tgtgcggttc tctgtctcct gggagcgggt
       61 gagttggtcc ccatggaaac gggagttacg cagacaccaa gacacctggt catgggaatg
      121 acaaataaga agtctttgaa atgtgaacaa catctgggtc ataacgctat gtattggtac
      181 aagcaaagtg ctaagaagcc actggagctc atgtttgtct acagtcttga agaacgggtt
      241 gaaaacaaca gtgtgccaag tcgcttctca cctgaatgcc ccaacagctc tcacttattc
      301 cttcacctac acaccctgca gccagaagac tcggccctgt atctctgtgt gagacgcagg
      361 gaagatggcc gcgaagaaaa cgtcatagct gtttcctgac tgaccattta aatcatacct
      421 gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt
      481 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      541 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      601 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      661 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      721 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      781 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      841 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      901 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      961 cctggcctat ttccctaaag ggtttattga gaatatgttt tttgtctcag ccaatccctg
     1021 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1081 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1141 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1201 gtactgcgat gagtggcagg gcggggcgta atgagggccc aaatgtaatc acctggctca
     1261 ccttcgggtg ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1321 gcatcacaaa aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1741 atttggtatc tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga
     1801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1861 cgcagaaaaa aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg
     1921 tgaaggtgag ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg
     1981 aatgatatca agcttgaatt cgtttgtaaa acgacggcca gtgctaaccg tatcgcattg
     2041 gccataccta ca
//
