LOCUS       sequence-468336-        2043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2043)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV5-7
FEATURES             Location/Qualifiers
     source          1..2043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(322..333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(373..389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      428..459
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        460..562
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             563..1222
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(629..648)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1246..1273
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1285..1872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1365..1384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1894..1923)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1996..2013
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1997..2013
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgggccccgg gctcctctgc tgggtgctgc tttgtcccct aggagaaggc
       61 ccagtggacg ctggagtcac ccaaagtccc acacacctga tcaaaacgag aggacagcac
      121 gtgactctga gatgctctcc tatctctggg cacaccagtg tgtcctcgta ccaacaggcc
      181 ctgggtcagg ggccccagtt tatctttcag tattatgaga aagaagagag aggaagagga
      241 aacttccctg atcaattctc aggtcaccag ttccctaact atagctctga gctgaatgtg
      301 aacgccttgt tgctagggga ctcggccctc tatctctgtg tgagacgcag ggaagatggc
      361 cgcgaagaaa acgtcatagc tgtttcctga ctgaccattt aaatcatacc tgacctccat
      421 agcagaaagt caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc
      481 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      541 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      601 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      661 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      721 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      781 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      841 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      901 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      961 tttccctaaa gggtttattg agaatatgtt ttttgtctca gccaatccct gggtgagttt
     1021 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1081 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1141 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1201 tgagtggcag ggcggggcgt aatgagggcc caaatgtaat cacctggctc accttcgggt
     1261 gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1321 aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1741 ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa
     1801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1861 aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgaaggtga
     1921 gccagtgagt tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc
     1981 aagcttgaat tcgtttgtaa aacgacggcc agtgctaacc gtatcgcatt ggccatacct
     2041 aca
//
