LOCUS       sequence-46834-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate C R1-R2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..180
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(221..526)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(323..342)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agcggccgcc ggcctcgagg gccggatccg aattcccggg agagctcgat atcgaaccac
       61 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      121 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      181 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
      241 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
      301 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
      361 tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca
      421 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
      481 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
      541 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
      601 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
      661 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
      721 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
      781 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
      841 ggcttttagt aagccggatc ctctagatta cgccccgcct gccactcatc gcagtactg
//
