LOCUS       sequence-468341-        2040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRBV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2040)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRBV6-8
FEATURES             Location/Qualifiers
     source          1..2040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(370..386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(370..386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      425..456
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        457..559
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             560..1219
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(626..645)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1243..1270
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1282..1869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1362..1381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1891..1920)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1993..2010
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1994..2010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcacca tgagcctcgg gctcctgtgc tgtgcggcct tttctctcct gtgggcaggt
       61 cccgtgaatg ctggtgtcac tcagacccca aaattccaca tcctgaagac aggacagagc
      121 atgacactgc agtgtgccca ggatatgaac catggataca tgtcctggta tcgacaagac
      181 ccaggcatgg ggctgagact gatttactac tcagctgctg ctggtacaac tgacaaagaa
      241 gtccccaatg gctacaatgt ctctagatta aacacagagg atttcccact caggctggtg
      301 tcggctgctc cctcccagac atctgtgtac ttgtgtgtga gacgcaggga agatggccgc
      361 gaagaaaacg tcatagctgt ttcctgactg accatttaaa tcatacctga cctccatagc
      421 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      481 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      541 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      601 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      661 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      721 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      781 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      841 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      901 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      961 ccctaaaggg tttattgaga atatgttttt tgtctcagcc aatccctggg tgagtttcac
     1021 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1081 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1141 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1201 gtggcagggc ggggcgtaat gagggcccaa atgtaatcac ctggctcacc ttcgggtggg
     1261 cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1321 tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1381 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1441 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1501 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1561 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1621 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1681 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1741 cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc cggcaaacaa
     1801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1861 ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg aaggtgagcc
     1921 agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgatatcaag
     1981 cttgaattcg tttgtaaaac gacggccagt gctaaccgta tcgcattggc catacctaca
//
