LOCUS       sequence-468351-       11925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the MS2-VP64-P65-HSF1 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Wu Y, Zhong A, Evangelisti A, Sidharta M, Danwei H, Studer L, Zhou T
  TITLE     Leveraging CRISPR activation for rapid assessment of gene editing 
            products in human pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2025 Apr 25:102499. doi: 
            10.1016/j.stemcr.2025.102499.
  PUBMED    40345204
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2025 Apr 25:102499. doi: 10.1016/j.stemcr.2025.102499."
COMMENT     Sequence Label: pLV-MVPH
FEATURES             Location/Qualifiers
     source          1..11925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..613
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             660..840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(858..1056)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(883..903)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1058..1437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1609..1628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1703..1807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1808..1876,1877..2668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2026..2045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2839..3427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3328..3347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3581..3598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3715..3736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3751..3756,3757..3774,3775..3781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3789..3805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3794..3816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3813..3829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3813..3829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3878..3999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3889..3908)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3943..3962
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4129..4503)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4522..4569)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4617..4946)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4631..4766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4685..4704)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4931..4951
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4960..5388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5070..5091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5282..5301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5434..5658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5647..5664
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5690..5870
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     6375..6391
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6376..6392
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6475..7653
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6706..7644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7601..7621
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7660..7669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7669..8055
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8110..8130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8155..8304
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8332..8874
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8899..9270
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9286..9339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9343..10362
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    10402..10990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10455..10475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10873..10884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    11043..11160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11458..11502)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11687..11920
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
       61 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
      121 tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt
      181 gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca
      241 cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa
      301 gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag
      361 gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt
      421 ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct
      481 taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg
      541 gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc
      601 tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact
      661 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
      721 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      781 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      841 agtacaggca aaacgcgctg cttatataga cctcccaccg tacacgccta ccgcccattt
      901 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
      961 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     1021 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     1081 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     1141 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     1201 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     1261 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     1321 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     1381 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     1441 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct
     1501 tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact
     1561 cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc
     1621 agattgtact gagagtgcac cataggggat cgggagatct cccgatccgt cgacgtcagg
     1681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2341 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2941 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3481 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3541 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3601 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3661 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3721 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3781 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     3841 caagctctag ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga
     3901 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     3961 gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag
     4021 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4081 caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc
     4141 ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca
     4201 cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac
     4261 gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt
     4321 gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac
     4381 cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa
     4441 ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc
     4501 catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta
     4561 attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg
     4621 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag
     4681 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     4741 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     4801 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     4861 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     4921 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
     4981 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5041 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5101 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5161 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5221 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5281 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5341 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
     5401 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     5461 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     5521 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     5581 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     5641 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct gctagagatt
     5701 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     5761 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     5821 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc
     5881 ggatgcagct ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat
     5941 acttctctct ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc
     6001 tctccttcat tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat
     6061 ccaaaggtca gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag
     6121 tagccttgtg tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt
     6181 agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     6241 gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac ccacctcctc ctcctcttgt
     6301 gcttctagcc aggcacaatc agcattggta gctgctgtat tgctacttgt gattgctcca
     6361 tgtttttcta ggtctcgagg tcgacggtat cgattgcaaa gatggataaa gttttaaaca
     6421 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     6481 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6541 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6601 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6661 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     6721 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     6781 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     6841 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     6901 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     6961 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     7021 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     7081 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     7141 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     7201 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     7261 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg aaggacgcgg
     7321 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     7381 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     7441 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     7501 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     7561 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     7621 caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatggc ttcaaacttt
     7681 actcagttcg tgctcgtgga caatggtggg acaggggatg tgacagtggc tccttctaat
     7741 ttcgctaatg gggtggcaga gtggatcagc tccaactcac ggagccaggc ctacaaggtg
     7801 acatgcagcg tcaggcagtc tagtgcccag aagagaaagt ataccatcaa ggtggaggtc
     7861 cccaaagtgg ctacccagac agtgggcgga gtcgaactgc ctgtcgccgc ttggaggtcc
     7921 tacctgaaca tggagctcac tatcccaatt ttcgctacca attctgactg tgaactcatc
     7981 gtgaaggcaa tgcaggggct cctcaaagac ggtaatccta tcccttccgc catcgccgct
     8041 aactcaggta tctacagcgc tggaggaggt ggaagcggag gaggaggaag cggaggagga
     8101 ggtagcggac ctaagaaaaa gaggaaggtg gcggccgctg gatccggacg ggctgacgca
     8161 ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt tgaccttgac
     8221 atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag tgacgccctt
     8281 gatgatttcg acctggacat gctgattaac tgtacaggca gtggaggatc cccttcaggg
     8341 cagatcagca accaggccct ggctctggcc cctagctccg ctccagtgct ggcccagact
     8401 atggtgccct ctagtgctat ggtgcctctg gcccagccac ctgctccagc ccctgtgctg
     8461 accccaggac caccccagtc actgagcgct ccagtgccca agtctacaca ggccggcgag
     8521 gggactctga gtgaagctct gctgcacctg cagttcgacg ctgatgagga cctgggagct
     8581 ctgctgggga acagcaccga tcccggagtg ttcacagatc tggcctccgt ggacaactct
     8641 gagtttcagc agctgctgaa tcagggcgtg tccatgtctc atagtacagc cgaaccaatg
     8701 ctgatggagt accccgaagc cattacccgg ctggtgaccg gcagccagcg gccccccgac
     8761 cccgctccaa ctcccctggg aaccagcggc ctgcctaatg ggctgtccgg agatgaagac
     8821 ttctcaagca tcgctgatat ggactttagt gccctgctgt cacagatttc ctctagtggg
     8881 cagggaggag gtggaagcgg cttcagcgtg gacaccagtg ccctgctgga cctgttcagc
     8941 ccctcggtga ccgtgcccga catgagcctg cctgaccttg acagcagcct ggccagtatc
     9001 caagagctcc tgtctcccca ggagcccccc aggcctcccg aggcagagaa cagcagcccg
     9061 gattcaggga agcagctggt gcactacaca gcgcagccgc tgttcctgct ggaccccggc
     9121 tccgtggaca ccgggagcaa cgacctgccg gtgctgtttg agctgggaga gggctcctac
     9181 ttctccgaag gggacggctt cgccgaggac cccaccatct ccctgctgac aggctcggag
     9241 cctcccaaag ccaaggaccc cactgtctcc tgtacaggca gtggagaggg cagaggaagt
     9301 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa ccatgaaaaa gcctgaactc
     9361 accgctacct ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     9421 cagctctccg agggcgaaga atctcgggct ttcagcttcg atgtgggagg gcgtggatat
     9481 gtcctgcggg tgaatagctg cgccgatggt ttctacaaag atcgctatgt ttatcggcac
     9541 tttgcatccg ccgctctccc tattcccgaa gtgcttgaca ttggggagtt cagcgagagc
     9601 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacct tgcaagacct gcctgaaacc
     9661 gaactgcccg ctgttctcca gcccgtcgcc gaggccatgg atgccatcgc tgccgccgat
     9721 cttagccaga ccagcgggtt cggcccattc ggacctcaag gaatcggtca atacactaca
     9781 tggcgcgatt tcatctgcgc tattgctgat ccccatgtgt atcactggca aactgtgatg
     9841 gacgacaccg tcagtgcctc cgtcgcccag gctctcgatg agctgatgct ttgggccgag
     9901 gactgccccg aagtccggca cctcgtgcac gccgatttcg gctccaacaa tgtcctgacc
     9961 gacaatggcc gcataacagc cgtcattgac tggagcgagg ccatgttcgg ggattcccaa
    10021 tacgaggtcg ccaacatctt cttctggagg ccctggttgg cttgtatgga gcagcagacc
    10081 cgctacttcg agcggaggca tcccgagctt gcaggatctc ctcggctccg ggcttatatg
    10141 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
    10201 gcttgggctc agggtcgctg cgacgcaatc gtccggtccg gagccgggac tgtcgggcgt
    10261 acacaaatcg cccgcagaag cgctgccgtc tggaccgatg gctgtgtgga agtgctcgcc
    10321 gatagtggaa acagacgccc cagcactcgt cctagggcaa aggatctgca gtaatgagaa
    10381 ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga aagattgact
    10441 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    10501 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    10561 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    10621 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    10681 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    10741 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    10801 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    10861 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    10921 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    10981 gcctccccgc atcgctagct aattaaccaa actggatctc tgctgtccct gtaataaacc
    11041 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
    11101 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
    11161 ttgtggatga atactgccat ttgtctgcag aattggcgca cgcagtgccg atccgttcac
    11221 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
    11281 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
    11341 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
    11401 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
    11461 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
    11521 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
    11581 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
    11641 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
    11701 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
    11761 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
    11821 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
    11881 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcc
//
