LOCUS       sequence-468361-       10347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which 
            contains 1 TRAF6 binding motif with a alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-1xA
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1130..1132,1133..1843)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1172..1193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1433..1452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1780..1801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1982..2570
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2035..2055)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2453..2464)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2573..2589)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2574..2590)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2680..2913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2938..2956)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2939..2956)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3246..3265)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3479..3498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3598..3620
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3658..3676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3744..3848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3849..3917,3918..4709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4067..4086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4880..5468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5369..5388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5622..5639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5709..5729)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5714..6043
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5894..6029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7177..7242
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7372..7392
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7817..7951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7854..7873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7908..7927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8119..8752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8799..8924
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9421..9654
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9839..9883
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10168..10285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgtctg cgggagaccc
      241 ccgcgtggga tccgggtccc tggactcctt catgttctcc atacccttgg tcgcgcttaa
      301 cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct cggacgcccg tggcggccga
      361 ctggaccttg ctggcggagg agatgggctt cgagtacttg gagatccgag agctggaaac
      421 gcgccctgac cccactcgca gtttgttgga tgcctggcag gggcgctctg gcgcgtctgt
      481 cggcaggctg ctagagctgc tggccttgtt agaccgtgag gatatactga aggagctgaa
      541 gtcgcgcatc gaggaggact gccagaaata cttaggtaag cagcagaacc aggagtccga
      601 gaagccttta caggtggcca gagtggaaag cagtgtccca caaacaaagg aactgggagg
      661 catcaccacc cttgatgacc ccctaggaca aacgccggaa cttttcgatg cctttatctg
      721 ctactgcccc aacgatatcg agtttgtgca ggagatgatc cggcaactag aacagacaga
      781 ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg ccgggcacct gtgtctggtc
      841 cattgccagc gagctaattg agaaaaggtg tcgccgcatg gtggtggttg tttctgacga
      901 ttatctacag agcaaggaat gtgacttcca gaccaagttt gcactcagcc tgtctccagg
      961 tgtccaacag aagcgactga ttcctattaa atacaaggcg atgaagaagg actttcccag
     1021 tatcctgcgg ttcatcacta tatgcgacta taccaaccct tgcaccaagt cctggttctg
     1081 gacccgcctt gccaaggctt tgtccctgcc cggaggtagt ggcgggtccg tgagcaaggg
     1141 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1201 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1261 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1321 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1381 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1441 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1501 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1561 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1621 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1741 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1801 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtgggg gctcaggtgg
     1861 aactgggtca ggtggcggtt ctggaggtcc aaaacaagaa ccacaggcga tagactttta
     1921 aagcggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     1981 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2041 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2101 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2161 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2221 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2281 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2341 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2401 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2461 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2521 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     2581 tcgacctcga gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     2641 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2701 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2881 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     2941 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     3001 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     3061 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     3121 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     3181 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     3241 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     3301 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     3361 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     3421 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     3481 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     3541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     3661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3841 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3901 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3961 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4021 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4081 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4141 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4201 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4261 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4321 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4381 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4441 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4501 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4561 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4621 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4681 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5521 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5581 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5641 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5701 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5761 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6001 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     6061 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     6121 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     6181 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     6241 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     6301 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     6361 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     6421 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     6481 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     6541 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     6601 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     6661 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     6721 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     6781 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     6841 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     6901 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     6961 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     7021 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     7081 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     7141 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     7201 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     7261 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     7321 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     7381 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     7441 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     7501 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     7561 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     7621 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     7681 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     7741 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7801 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7921 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     7981 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     8041 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     8101 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     8161 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     8221 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     8281 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     8341 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     8401 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     8461 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     8521 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     8581 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8641 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8701 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8761 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8821 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8881 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8941 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     9001 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9061 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9121 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9181 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9241 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     9301 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9361 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9421 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9481 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9541 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9601 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9661 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     9721 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9781 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9841 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9901 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9961 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10021 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    10081 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
    10141 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
    10201 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    10261 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
    10321 gatcgataag cttgatatcg aattcag
//
