LOCUS       sequence-468363-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which 
            contains only 5 TRAF6 binding motifs with a alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-5xA
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..212
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      71..206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1131..1133,1134..1844)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1173..1194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1434..1453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1781..1802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2247..2835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2300..2320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2718..2729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2838..2854)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2839..2855)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2945..3178
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3203..3221)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3204..3221)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3511..3530)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3744..3763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3863..3885
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3923..3941)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4009..4113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4114..4182,4183..4974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4332..4351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5145..5733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5634..5653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5887..5904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5974..5994)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5979..6308
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6159..6294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7442..7507
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7637..7657
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8082..8216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8119..8138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8173..8192
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8384..9017
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9064..9189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9686..9919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10104..10148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10433..10550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
       61 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      121 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
      181 gtagtgagga ggcttttttg gaggcctagg ctacgcgtgc caccatgtct gcgggagacc
      241 cccgcgtggg atccgggtcc ctggactcct tcatgttctc catacccttg gtcgcgctta
      301 acgtgggagt gaggcgccgc ctatcgctgt tcttgaaccc tcggacgccc gtggcggccg
      361 actggacctt gctggcggag gagatgggct tcgagtactt ggagatccga gagctggaaa
      421 cgcgccctga ccccactcgc agtttgttgg atgcctggca ggggcgctct ggcgcgtctg
      481 tcggcaggct gctagagctg ctggccttgt tagaccgtga ggatatactg aaggagctga
      541 agtcgcgcat cgaggaggac tgccagaaat acttaggtaa gcagcagaac caggagtccg
      601 agaagccttt acaggtggcc agagtggaaa gcagtgtccc acaaacaaag gaactgggag
      661 gcatcaccac ccttgatgac cccctaggac aaacgccgga acttttcgat gcctttatct
      721 gctactgccc caacgatatc gagtttgtgc aggagatgat ccggcaacta gaacagacag
      781 actatcggct taagttgtgt gtgtccgacc gtgacgtcct gccgggcacc tgtgtctggt
      841 ccattgccag cgagctaatt gagaaaaggt gtcgccgcat ggtggtggtt gtttctgacg
      901 attatctaca gagcaaggaa tgtgacttcc agaccaagtt tgcactcagc ctgtctccag
      961 gtgtccaaca gaagcgactg attcctatta aatacaaggc gatgaagaag gactttccca
     1021 gtatcctgcg gttcatcact atatgcgact ataccaaccc ttgcaccaag tcctggttct
     1081 ggacccgcct tgccaaggct ttgtccctgc ccggaggtag tggcgggtcc gtgagcaagg
     1141 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1201 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1261 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1321 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1381 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1441 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1501 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1561 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1621 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1681 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1741 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1801 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtgga agtcctaaac
     1861 aagaacctca agcgatcgat ttcggaggag gcagtggcgg cacaggatcc ggcgggggca
     1921 gcggtgggcc caaacaggaa ccacaggcaa ttgatttcgg cggcgggtct ggaggtacag
     1981 gtagcggggg cactggaggc tctccaaagc aggagcctca ggcgatagac tttggcgggg
     2041 ggagcggcgg tactggatct ggcggtggca gtggtggccc taagcaggag ccccaagcaa
     2101 tcgactttgg gggaggcagt ggtgggaccg gatcaggagg aactgggggt agtcccaagc
     2161 aagaaccaca agcgatcgac ttttaaagcg gccgcgactc tagagtcgac ctgcaggcat
     2221 gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2281 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2341 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2401 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2461 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2521 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2581 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2641 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2701 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2761 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2821 gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct
     2881 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2941 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     3001 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3061 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3121 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     3181 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     3241 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     3301 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     3361 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     3421 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     3481 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     3541 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     3601 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     3661 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     3721 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3781 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3841 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3901 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3961 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4321 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5941 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     6001 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6061 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6121 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6241 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6301 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     6361 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     6421 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     6481 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     6541 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     6601 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     6661 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     6721 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     6781 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     6841 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     6901 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     6961 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     7021 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     7081 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     7141 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     7201 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     7261 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     7321 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     7381 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     7441 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     7501 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     7561 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     7621 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     7681 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     7741 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     7801 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     7861 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     7921 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     7981 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     8041 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     8101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     8221 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     8281 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     8341 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
     8401 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     8461 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     8521 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     8581 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     8641 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     8701 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8761 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8821 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9001 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     9061 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     9121 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9181 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9241 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9301 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9361 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9421 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9481 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     9541 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9601 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9661 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9721 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9781 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9841 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9901 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9961 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10021 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10081 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10141 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10201 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10261 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    10321 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    10381 gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa
    10441 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10501 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    10561 acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc a
//
