LOCUS       sequence-468365-       10599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with 
            alanine mutation in the TRAF6 binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-20xTA-3xAla
FEATURES             Location/Qualifiers
     source          1..10599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1457..1459,1460..2173)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1499..1520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1760..1779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2107..2128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2234..2822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2287..2307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2705..2716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2825..2841)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2826..2842)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2932..3165
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3190..3208)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3191..3208)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3498..3517)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3731..3750)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3850..3872
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3910..3928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3996..4100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4101..4169,4170..4961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4319..4338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5132..5720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5621..5640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5874..5891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5961..5981)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5966..6295
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6146..6281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7429..7494
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7624..7644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8069..8203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8106..8125)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8160..8179
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8371..9004
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9051..9176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9673..9906
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10091..10135
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10420..10537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgactg ctaccgccac
      241 agctactgca actgccacgg ccacagccac ggccaccgcg actgcaactg caaccgctac
      301 tgctactgcc acagctaccg caactgctac tgccacagcg actgccggag gatcaggcgg
      361 atcatccgaa aagcctttac aggtggccag agtggaaagc agtgtcccac aaacaaagga
      421 actgggaggc atcaccaccc ttgatgaccc cctaggacaa acgccggaac ttttcgatgc
      481 ctttatctgc tactgcccca acgatatcga gtttgtgcag gagatgatcc ggcaactaga
      541 acagacagac tatcggctta agttgtgtgt gtccgaccgt gacgtcctgc cgggcacctg
      601 tgtctggtcc attgccagcg agctaattga gaaaaggtgt cgccgcatgg tggtggttgt
      661 ttctgacgat tatctacaga gcaaggaatg tgacttccag accaagtttg cactcagcct
      721 gtctccaggt gtccaacaga agcgactgat tcctattaaa tacaaggcga tgaagaagga
      781 ctttcccagt atcctgcggt tcatcactat atgcgactat accaaccctt gcaccaagtc
      841 ctggttctgg acccgccttg ccaaggcttt gtccctgccc ggaggtagtg gcgggtcccc
      901 tccttctccc caagccaact cctatgtcag ctccactggt agggctcact ccggagccgc
      961 accatggcag ccactcgctg caccatcagg tgcttcagcc caggccgcag agcaactgca
     1021 acggggaccc aaccagccag tcgcgtccga cgaaagtctt ggagggctga gcgctgctct
     1081 gcggtcctgg caccttacac caagttgtcc ccttgatcct gctcctctgc gggaggctgg
     1141 gtgcccccaa ggtgacacag caggggaatc ctcatggggt tcaggacctg ggagccggcc
     1201 aaccgctgtt gaaggcctgg ctttgggctc ctctgcatca tcatcatcag aacctcccca
     1261 gattatcatc aatccagctc gccagaaaat ggttcagaaa ttggctttgt atgaggatgg
     1321 tgccctggat tccttgcaat tgctgtcatc ttcttctctt cccggacttg gacttgaaca
     1381 agacagacaa ggtccagagg cgtccgacga gttccagagc ggaggttccg ggggtactgg
     1441 gggtagtgga ggaagtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1501 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1561 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1621 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1681 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1741 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1801 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1861 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1921 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1981 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2041 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     2101 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2161 gctgtacaag taaagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     2221 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2281 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2341 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2401 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2461 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2521 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2581 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2641 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2701 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2761 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2821 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     2881 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2941 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     3181 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     3241 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     3301 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     3361 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     3421 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     3481 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     3541 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     3601 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     3661 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     3721 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3781 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3841 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3901 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3961 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4321 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4621 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5941 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6001 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6121 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6301 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     6361 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     6421 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     6481 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     6541 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     6601 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     6661 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     6721 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     6781 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     6841 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     6901 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     6961 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     7021 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     7081 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     7141 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     7201 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     7261 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     7321 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     7381 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     7441 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     7501 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     7561 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     7621 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     7681 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     7741 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     7801 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     7861 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     7921 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     7981 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     8041 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     8101 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8161 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     8221 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     8281 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     8341 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     8401 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     8461 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     8521 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     8581 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     8641 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     8701 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     8761 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     8821 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8881 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8941 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9001 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     9061 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     9121 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9181 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9241 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9301 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9361 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9421 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9481 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     9541 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9601 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9661 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9721 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9781 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9841 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9901 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9961 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10021 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10081 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10141 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10201 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10261 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    10321 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    10381 atctcgacgg tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    10441 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    10501 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    10561 agatccagtt tggatcgata agcttgatat cgaattcag
//
