LOCUS       sequence-468408-        8028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuorescent reporter encoding H2B-GFP fusion under control of E1b 
            minimal promoter with eight IKZF1 binding sites upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW410 IKZF1tar x8-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..8028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   428..462
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(953..969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(953..969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(966..988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    977..993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1001..1007,1008..1025,1026..1031))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1046..1067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1184..1201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1355..1943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1435..1454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2114..2905,2906..2974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2737..2756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2975..3079)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3105..3560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3242..3263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3454..3473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3687..3709
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3701..3718
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3702..3718
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3848..3910
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           4568..4610
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             4659..5036
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(5055..5057,5058..5768)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5796..5813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5802..6009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6042..6062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6047..6376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6227..6362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6289..6308
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6425..7450
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6425..7450
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7510..7530)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7572..7693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7609..7628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7663..7682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
       61 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
      121 catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata
      181 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
      241 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
      301 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
      361 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
      421 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag
      481 gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc
      541 atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt
      601 gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt
      661 cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat
      721 accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc
      781 aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc
      841 ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa
      901 tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc
      961 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1021 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1081 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1141 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2401 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2701 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     3121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3181 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3301 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3481 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3541 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3601 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3661 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3721 gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa
     3781 tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct
     3841 agattaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc atgtgtaaaa
     3901 ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat
     3961 taagttttat tatatttaca cttacatact aataataaat tcaacaaaca atttatttat
     4021 gtttatttat ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
     4081 tttatgaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     4141 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatccca
     4201 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4261 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaggc
     4321 ctccacggcc acaattgacg ataaacagga aactcttcaa acaggaaaga ggtcaaacag
     4381 gaaacagaac aaacaggaaa gccttcaaac aggaaaaagg tcaaacagga aatcgtacaa
     4441 acaggaaacg tatcagtaaa caggaaacgt atcagtctag agggtatata atggatccca
     4501 tcgcgtctca gcctcacttt gagctcctcc acacgaattc cccgataccg tcgattcaag
     4561 gagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatctacc
     4621 aagcttggta ccgagctcgg atccaactcg aggccaccat gccagagcca gcgaagtctg
     4681 ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca
     4741 agaagcgcaa gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc
     4801 aggtccaccc tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga
     4861 acgacatttt cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct
     4921 cgaccatcac ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg
     4981 ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc
     5041 cccgggtacc ggtcgtctca aagggcgagg aactttttac gggcgttgtt ccgatcctcg
     5101 tcgaattgga cggggacgtt aatgggcata aattctcagt tagtggtgag ggtgaggggg
     5161 acgcgacata tgggaaactt acgctgaagt tcatatgtac caccggaaaa ttgccggtgc
     5221 cgtggcctac tctcgtcacc accctgacat atggtgttca gtgttttagc cggtatccag
     5281 accatatgaa acagcacgat ttttttaaat ccgctatgcc tgaaggttac gtacaggagc
     5341 gaacgatatt ttttaaagac gacggaaact acaaaactag agccgaggta aaattcgagg
     5401 gtgacactct cgtgaaccgg atcgaactga agggcataga ttttaaggag gatggcaata
     5461 tactgggcca caagctcgaa tacaactata attctcataa tgtctatatc atggcggaca
     5521 aacagaaaaa cggcatcaag gtaaacttta aaatccgaca caatattgag gacggctctg
     5581 tccagcttgc ggaccattac caacagaaca cgccgatagg ggatggtcca gtacttctcc
     5641 ctgataatca ctatttgtct actcagtcag ctcttagcaa ggacccaaac gaaaaacggg
     5701 atcacatggt ccttcttgag ttcgtcactg cggctgggat aacactcggg atggacgaac
     5761 tgtataagta gctagcgaat tcgaatttaa atcggcctcg actgtgcctt ctagttgcca
     5821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6001 tgctggggac gcggccgcaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6061 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6121 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6181 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6301 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6361 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6421 cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     6481 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     6541 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     6601 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     6661 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     6721 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     6781 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     6841 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     6901 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     6961 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     7021 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     7081 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     7141 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     7201 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     7261 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     7321 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     7381 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     7441 aaaggaatag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7501 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7561 tgcgttaact aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7681 tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc
     7741 tcatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt
     7801 cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct
     7861 cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg
     7921 attccttttt tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag
     7981 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccac
//
