LOCUS       sequence-468428-        8078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuorescent reporter encoding H2B-GFP fusion under control of E1b 
            minimal promoter with eight ZNF250 binding sites upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW409 ZNF250tarv3 x8-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..8078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   434..468
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(959..975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(959..975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(972..994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    983..999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1007..1013,1014..1031,1032..1037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1052..1073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1190..1207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1361..1949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1441..1460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2120..2911,2912..2980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2743..2762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2981..3085)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3111..3566)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3248..3269)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3460..3479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3693..3715
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3707..3724
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3708..3724
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3854..3916
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           4624..4666
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             4715..5092
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(5111..5113,5114..5824)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5852..5869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5858..6065
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6098..6118)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6103..6432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6283..6418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6345..6364
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6481..7506
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6481..7506
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7566..7586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7628..7749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7665..7684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7719..7738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
       61 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
      121 taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa caagaaaata
      181 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
      241 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
      301 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
      361 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
      421 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
      481 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
      541 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
      601 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
      661 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
      721 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
      781 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
      841 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
      901 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
      961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2101 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2221 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     3121 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3181 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3241 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3301 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3361 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3421 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3481 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3541 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     3601 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3661 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3721 cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg
     3781 tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac
     3841 gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgt
     3901 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
     3961 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     4021 atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     4081 aaaactttta tgagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     4141 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     4201 atcccagagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     4261 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     4321 aaaggcctcc acggccacaa ttgacgattg agtaacactg tatctagtct acctacaggg
     4381 ctcaaaattg caaggctctt caaaattgca agggaggtca aaattgcaag gcagaacaaa
     4441 attgcaaggg ccttcaaaat tgcaaggaag gtcaaaattg caaggtcgta caaaattgca
     4501 aggcgtatca gaaaattgca aggcgtatca gtctagaggg tatataatgg atcccatcgc
     4561 gtctcagcct cactttgagc tcctccacac gaattccccg ataccgtcga ttcaaggagc
     4621 ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga tctaccaagc
     4681 ttggtaccga gctcggatcc aactcgaggc caccatgcca gagccagcga agtctgctcc
     4741 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     4801 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     4861 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     4921 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     4981 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     5041 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccccg
     5101 ggtaccggtc gtctcaaagg gcgaggaact ttttacgggc gttgttccga tcctcgtcga
     5161 attggacggg gacgttaatg ggcataaatt ctcagttagt ggtgagggtg agggggacgc
     5221 gacatatggg aaacttacgc tgaagttcat atgtaccacc ggaaaattgc cggtgccgtg
     5281 gcctactctc gtcaccaccc tgacatatgg tgttcagtgt tttagccggt atccagacca
     5341 tatgaaacag cacgattttt ttaaatccgc tatgcctgaa ggttacgtac aggagcgaac
     5401 gatatttttt aaagacgacg gaaactacaa aactagagcc gaggtaaaat tcgagggtga
     5461 cactctcgtg aaccggatcg aactgaaggg catagatttt aaggaggatg gcaatatact
     5521 gggccacaag ctcgaataca actataattc tcataatgtc tatatcatgg cggacaaaca
     5581 gaaaaacggc atcaaggtaa actttaaaat ccgacacaat attgaggacg gctctgtcca
     5641 gcttgcggac cattaccaac agaacacgcc gataggggat ggtccagtac ttctccctga
     5701 taatcactat ttgtctactc agtcagctct tagcaaggac ccaaacgaaa aacgggatca
     5761 catggtcctt cttgagttcg tcactgcggc tgggataaca ctcgggatgg acgaactgta
     5821 taagtagcta gcgaattcga atttaaatcg gcctcgactg tgccttctag ttgccagcca
     5881 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5941 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6001 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6061 ggggacgcgg ccgcaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     6121 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6181 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6241 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6361 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6421 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     6481 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6541 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6601 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     6661 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     6721 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     6781 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6841 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6901 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6961 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     7021 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     7081 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7141 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     7201 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     7261 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     7321 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     7381 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7441 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     7501 gaataggtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7561 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg
     7621 ttaactaaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat
     7801 ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca
     7861 aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca
     7921 ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc
     7981 cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     8041 aaagagcagc gagaagcgtt cagaggaaag cgatcccg
//
