LOCUS       sequence-468432-       10841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the 3' UTR in regulating ALDH5A1-P2 
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10841)
  TITLE     Study the role and mechanism of the 3' UTR in regulating gene 
            function.
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ALDH5A1-P2
FEATURES             Location/Qualifiers
     source          1..10841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8188..9840)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      9837..9846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9887..10386)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9935..9954)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10328..10351
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    10443..10726
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttac agggagagga aattctgtta
     4681 attccacgtt tattaatcac acagctctct gcagactaga cactaaaaca cacaattgtc
     4741 aaaaactaga aaaatgagtt atgtccacgt tttaaaagca aaactttata aatttcttac
     4801 cacactcatt cccaagtttt atcccacaaa gtatagcatg aaacaatgac aacatacata
     4861 ttattcaagt aaaatgctat ttaaaatagc tgcacacagg taattaaaac actaggatcc
     4921 agtttttaga ggaaaaagtc atgtggcaca atttcaagtt cataattgaa gttaacagta
     4981 aaacagattt gctcacattt gcttctgatc tttatttctg ctgtctctct ttagtagaat
     5041 aaagaaatgg cacttcacat aaaatcatat ttaaaagcta ctaaaatgga taaacagatg
     5101 cagatcagct ctttaatgag aatccctgca tagctggagg aagttcaagg ataattgttt
     5161 tttcaaggca aatagactct tgttacgtgg ctaatttcat gtacattatc atcgctggga
     5221 agttcctgct gagaactcac ggtagctatg ccagaaaagt actttcttca caggaggtgt
     5281 tgaaataatg ccggataaat gcaagtaagg tcaatatctc cctcttggtc tagatcagga
     5341 gctggcaaac tttttcttta aggagacaag tagtaaatct tttaggcttt gcaggctata
     5401 ctgtccctgt cccaactatt caactctgcc aagaaagcag tcccagacaa taccataaat
     5461 gaatgggtgt ggctatgctc taataacatt ttgtttatgg acactgaaat ctgaatttca
     5521 tagaattttc aggtgtcatg aaatattgtt cttcttttga ttttctgccc aatcatttga
     5581 aaacatataa accattctta gctcacaggt tgtataaaaa caggtggcag gccaggtttg
     5641 attcgtgggc catagtttgc caacccctga tctaatggtc cttttctagt ccatgttgta
     5701 aaatgtatat atttttaaaa tcccgttaca tatggctact ttatttaaaa aacaacaaaa
     5761 acgttcagtt aaaaataatt ctctttcttc ccacaaccaa gggccatttt actaaacaat
     5821 aagctatttc ctttaattag aaaattgatc aaggatatac aatgagtctc tggcctcaat
     5881 ttatgaaccc atgagccaaa catgcaagaa gactcaaaat ttgccaccca gccaaagaat
     5941 ctactggctt acaatgttaa aaatttattt ggaagtattc ctgcacacat ctcagcatcg
     6001 gtaatccaga gttataaaaa ataatgttgg agcatttgta ttcttttttt gaggcggagt
     6061 ctcgctctgt catccaggct ggagtgcagt ggcgtgatct ctgctcactg caagctccgc
     6121 ctcctgggtt cacgccattc tcctgcctca gcctccagag tagctgggac tacaggcacc
     6181 tgccaccaca tccgactaat tttttttgta tttttagtag agacggggtt tcaccgtgtt
     6241 agccaggatg gtctcgatct cctgatgtcg tgatctgcct gccttggcct cccaaggtgt
     6301 tgggattaca ggtgtgagcc actgtgccca gcccagcatt tgtattctta atagaaaaag
     6361 gatggataca tctaaatcac aagtaattaa aatgttatat aaaaccacct aaaaactaca
     6421 caaataaaga gaagacatta actgtcaaaa tgctgagtat gtgattcttg acagggccgg
     6481 ggcactgtca ctaggagatg aatttcagct cttccttctc cttgggacct tgggaatgtg
     6541 gctagtacac tccaggccag atgctaggca gctccctgtg tgcccagggt cttgccagac
     6601 ctttagttac tcaaccatag cttcccagtg gctcaatgag ggatgttaaa tcctaggtgg
     6661 ggttcaggag taattgcatc cagcactact ttcattcaca ttttccttcc acaacccaga
     6721 ataaccacaa gatgtaagtg gagtctacac agacagagat ggggaaagga aggtgcttcg
     6781 tctccaccta cttggccgcc taattggatt tgcatctgtt taagattacg gagtctttct
     6841 ttcaaagtga gaggcaacgc cggtcatagc ggcttttgtt ttttgtgcgt tatttgaatg
     6901 atgagctgta aaaactctca ttagataaaa ggtgcctgaa attcaagggc tcatgcctct
     6961 tctacaaagt gggttggcaa ttacagaaat tctttccctt ttgggttgac tgtggtgaaa
     7021 gtacttttgc tcttttggag gtgagggagc agcaccacac caggacagaa agaacaggct
     7081 caggtgacag ccacttactc aggctcaggt cggtgggcaa gtgggcactg gcctcctccc
     7141 atcttggcaa agccactgac agtaggcaag gaagggctgg gcggtgctgc tcgtaacaat
     7201 ggctcttgta tgtccttaga attttcattt ttttgtgaag tgcttttact ttctctctgg
     7261 ctcacagtat cgcaaggagc taagtaaggc ctgtatggaa tctccactgt atccatggga
     7321 tgggaggtgc tgaacacacg cccaaggcaa caaggccact cagggaagga gccaggccat
     7381 gccactgcgt gccttccatc ctaccaagct gcttctgcta ttctttgctt tacacttcag
     7441 ctcatttact caaggaaatg aaatgaagtg gcaaacagga cagaaatgaa aatagaattc
     7501 tttaagtggt aaatttggga caataaaggc atcaattaaa atatggttca ggaataggga
     7561 gaggtgctcc ttcgtgcctc acagaagcac ggacaattct gtgctattta ttgtctttga
     7621 aagagggttc actcaaacct ctgccgcaac acctccagat gcacattcgg tgtcttctgt
     7681 gctgagctgg cagatgccgg ggaggggtgc tgtgcccctc acctgcctgc cagtcacagt
     7741 caagggtctc cttcaaggtg ctctgggccc atgttctgta gcgacatggg ggatggttgt
     7801 gccactgccc aggcagtgat caagcctttg tgacggccca tggggctggg cctgccttgt
     7861 gctgtgcctg ctctgaggca gctggggctg tggtggggag ccagggcctc attctgtaaa
     7921 cccagtgctg gttctctggg agtctgcagc cacaggcacg tggcacatat tggtccctag
     7981 ttacattaag ggcaggggta ggatttgcat ctgtttaaga ttacaaggac tggatgagtt
     8041 ctgaaaaatt cataattctg aaaacctatt agtttattta aaataatgga tggcatgtac
     8101 ctatatatgt agataagtct ccttttaaat ttttttaaag aaccaaagaa tcactcgagg
     8161 ctagcgagct cgtttaaaca actagaatta cacggcgatc ttgccgccct tcttggcctt
     8221 aatgagaatc tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt taggcacctc
     8281 gtccacgaac acaacaccac cgcgcagctt cttggcggtt gtaacctggc tggccacata
     8341 gtccacgatc tccttctcgg tcatggtttt accgtgttcc agcacgacga ctgcggcggg
     8401 cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga tgttggggtg
     8461 ttgcagcagg atgctctcca gttcggctgg ggctacctgg tagcccttgt atttgatcag
     8521 gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg cgatgtcgcc
     8581 gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta gcctcggggt tgttaacgta
     8641 gccgctcatg atcatggggc cacggacgca cagctcgccg cgctggttca cacccagtgt
     8701 cttaccggtg tccaagtcca ccaccttagc ctcgaagaag ggcaccacct tgcctactgc
     8761 gccaggcttg tcgtcccctt cgggggtgat cagaatggcg ctggttgttt ctgtcaggcc
     8821 gtagccctgg cggatgcctg gtaggtggaa gcgtttggcc acggcctcac ctacctcctt
     8881 gctgagcggc gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt acttgtcgat
     8941 gagagtgctc ttagcgaaga agctaaatag tgtgggcacc agcagggcag attgaatctt
     9001 atagtcttgc aagctgcgca agaatagctc ctcctcgaag cggtacatga gcacgacccg
     9061 aaagccgcag atcaagtagc ccagcgtggt gaacatgccg aagccgtggt gaaatggcac
     9121 cacgctgagg atagcggtgt cggggatgat ctggttgccg aagatggggt cgcgggcatg
     9181 actgaatcgg acacaagcgg tgcggtgcgg tagggctacg cccttgggca atccggtact
     9241 gccactactg ttcatgatca gggcgatggt tttgtcccgg tcgaagctct cgggcacgaa
     9301 gtcgtactcg ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca tgctttggaa
     9361 gccctggtag tcggtcttgc tatccatgat gatgatcttt tgtatgatcg gtagcttctt
     9421 ttgcacgttg aggatctttt gcagcccttt cttgctcacg aatacgacgg tgggctggct
     9481 gatgcccatg ctgttcagca gctcgcgctc gttgtagatg tcgttagctg gggccacagc
     9541 cacaccgatg aacagggcac ccaacacggg catgaagaac tgcaagctat tctcgctgca
     9601 caccacgatc cgatggtttg tattcagccc atagcgcttc atagcttctg ccagccgaac
     9661 gctcatctcg aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt cggtaaaggc
     9721 gatggtgccg ggcaccaggg cgtagcgctt catggctttg tgcagctgct cgccggcggt
     9781 cccgtcttcg agtgggtaga atggcgctgg gcccttctta atgtttttgg catcttccat
     9841 ggtggcttta ccaacagtac cggattgcca agcttgggct gcaggtcgaa aggcccggag
     9901 atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc
     9961 ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg
    10021 acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc
    10081 tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag
    10141 tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga
    10201 acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg
    10261 gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc
    10321 aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc
    10381 ctacccggta gatctcgatc ctctacgccg gacgcatcgt ggccggagta ctgcggttta
    10441 tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca
    10501 ccgctaaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac
    10561 ccgaaaaact gccgcaatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca
    10621 gagggtacag gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc
    10681 accatcccag cgaagggcca tccagcgtgc gttcctgtat ttccggacta taaaaggatc
    10741 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10801 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
