LOCUS       sequence-468433-        8050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuorescent reporter encoding H2B-GFP fusion under control of E1b 
            minimal promoter with four ZNF35/ZNF250 binding sites upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..8050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(169..960,961..1029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     792..811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1030..1134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1160..1615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1297..1318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1509..1528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1742..1764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1756..1773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1757..1773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1903..1965
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           2645..2687
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2736..3113
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(3132..3134,3135..3845)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3873..3890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3879..4086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4119..4139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4124..4453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4304..4439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4366..4385
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4502..5527
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4502..5527
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5587..5607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5649..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5686..5705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5740..5759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6533..6567
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7058..7074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7058..7074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7071..7093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7082..7098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7106..7112,7113..7130,7131..7136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7151..7172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7289..7306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7460..8048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7540..7559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     1201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     1621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     1801 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     1861 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     1921 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     1981 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     2041 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     2101 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     2161 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     2221 gctccgctcc ggtccggcgc tccccccgca tcccagagcc ggcagcgtgc ggggacagcc
     2281 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     2341 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccacaat tgacgattga
     2401 gtaacactgt atctagtcta cctacagggc tcatatataa agagatatat aaagagctct
     2461 tcatatataa agagatatat aaagaggagg tcatatataa agagatatat aaagagcaga
     2521 acatatataa agagatatat aaagagtatc agtctagagg gtatataatg gatcccatcg
     2581 cgtctcagcc tcactttgag ctcctccaca cgaattcccc gataccgtcg attcaaggag
     2641 cttgcttgtt ctttttgcag aagctcagaa taaacgctca actttggcag atctaccaag
     2701 cttggtaccg agctcggatc caactcgagg ccaccatgcc agagccagcg aagtctgctc
     2761 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
     2821 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
     2881 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
     2941 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
     3001 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
     3061 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatcccc
     3121 gggtaccggt cgtctcaaag ggcgaggaac tttttacggg cgttgttccg atcctcgtcg
     3181 aattggacgg ggacgttaat gggcataaat tctcagttag tggtgagggt gagggggacg
     3241 cgacatatgg gaaacttacg ctgaagttca tatgtaccac cggaaaattg ccggtgccgt
     3301 ggcctactct cgtcaccacc ctgacatatg gtgttcagtg ttttagccgg tatccagacc
     3361 atatgaaaca gcacgatttt tttaaatccg ctatgcctga aggttacgta caggagcgaa
     3421 cgatattttt taaagacgac ggaaactaca aaactagagc cgaggtaaaa ttcgagggtg
     3481 acactctcgt gaaccggatc gaactgaagg gcatagattt taaggaggat ggcaatatac
     3541 tgggccacaa gctcgaatac aactataatt ctcataatgt ctatatcatg gcggacaaac
     3601 agaaaaacgg catcaaggta aactttaaaa tccgacacaa tattgaggac ggctctgtcc
     3661 agcttgcgga ccattaccaa cagaacacgc cgatagggga tggtccagta cttctccctg
     3721 ataatcacta tttgtctact cagtcagctc ttagcaagga cccaaacgaa aaacgggatc
     3781 acatggtcct tcttgagttc gtcactgcgg ctgggataac actcgggatg gacgaactgt
     3841 ataagtagct agcgaattcg aatttaaatc ggcctcgact gtgccttcta gttgccagcc
     3901 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3961 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4021 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4081 tggggacgcg gccgcaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4201 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4381 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4441 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     4501 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     4561 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     4621 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     4681 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     4741 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     4801 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     4861 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     4921 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     4981 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     5041 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     5101 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     5161 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     5221 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     5281 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     5341 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     5401 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     5461 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     5521 ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5581 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5641 gttaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5761 atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctca
     5821 tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc
     5881 aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac
     5941 aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt
     6001 ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct
     6061 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
     6121 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     6181 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
     6241 tggggggggg ctgtccctga tatctataac aagaaaatat atatataata agttatcacg
     6301 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     6361 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     6421 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     6481 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     6541 aattttacgc agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg
     6601 tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa
     6661 gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc
     6721 tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc
     6781 atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg
     6841 cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc
     6901 ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga
     6961 tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc
     7021 cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7081 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7141 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7201 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7861 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8041 gatctcaaga
//
