LOCUS       sequence-468447-       10346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac-integrated plasmid containing hygromycin resistance gene, 
            ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H...
FEATURES             Location/Qualifiers
     source          1..10346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(169..960,961..1029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     792..811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1030..1134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1160..1615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1297..1318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1509..1528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1742..1764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1756..1773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1757..1773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1903..1965
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           2653..2695
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2744..3121
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3140..3847
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     polyA_signal    3881..4088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4121..4141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4126..4455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4306..4441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4368..4387
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4504..5529
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4504..5529
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5589..5609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5651..5772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5688..5707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5742..5761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        6267..6646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6647..6850
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6801..6821
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6852..6870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6873..6891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6901..6925
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7017..7026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7032..7034,7035..7745)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             8259..8288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    8350..8557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8829..8863
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9354..9370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9354..9370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9367..9389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9378..9394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9402..9408,9409..9426,9427..9432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9447..9468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9585..9602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9756..10344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9836..9855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     1201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     1621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     1801 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     1861 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     1921 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     1981 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     2041 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     2101 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     2161 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     2221 gctccgctcc ggtccggcgc tccccccgca tcccagagcc ggcagcgtgc ggggacagcc
     2281 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     2341 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccacaat tgacgattga
     2401 gtaacactgt atctagtcta cctacagggc tcaaaattgc aaggatatat aaagagctct
     2461 tcaaaattgc aaggatatat aaagaggagg tcaaaattgc aaggatatat aaagagcaga
     2521 acaaaattgc aaggatatat aaagaggcct tccgtatcag tctagagggt atataatgga
     2581 tcccatcgcg tctcagcctc actttgagct cctccacacg aattccccga taccgtcgat
     2641 tcaaggagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat
     2701 ctaccaagct tggtaccgag ctcggatcca actcgaggcc accatgccag agccagcgaa
     2761 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     2821 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     2881 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt
     2941 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     3001 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     3061 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     3121 ggatccccgg gtaccggtcg tgtctaaggg cgaagagctg attaaggaga acatgcacat
     3181 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     3241 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     3301 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3361 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     3421 gagagtcacc acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca
     3481 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     3541 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga
     3601 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     3661 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     3721 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg aaacctacgt
     3781 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     3841 gcttaattag ctagcgaatt cgaatttaaa tcggcctcga ctgtgccttc tagttgccag
     3901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4081 gctggggacg cggccgcaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4381 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4441 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     4501 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     4561 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     4621 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     4681 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     4741 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     4801 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     4861 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4921 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     4981 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5041 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5101 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5161 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     5221 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     5281 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     5341 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     5401 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     5461 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     5521 aaggaatagg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5641 gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5701 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5761 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     5821 catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     5881 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     5941 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     6001 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     6061 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     6121 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     6181 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     6241 tttggggggg ggctgtccct gatgttgaca ttgattattg actagttatt aatagtaatc
     6301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6841 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     6901 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     6961 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttcgcca
     7021 ccatgggatc cgtctcaaag ggcgaggaac tttttacggg cgttgttccg atcctcgtcg
     7081 aattggacgg ggacgttaat gggcataaat tctcagttag tggtgagggt gagggggacg
     7141 cgacatatgg gaaacttacg ctgaagttca tatgtaccac cggaaaattg ccagtgccgt
     7201 ggcctactct cgtcaccacc ctgacatatg gtgttcagtg ttttagccgg tatccagacc
     7261 atatgaaaca gcacgatttt tttaaatccg ctatgcctga aggttacgta caggagcgaa
     7321 cgatattttt taaagacgac ggaaactaca aaactagagc cgaggtaaaa ttcgagggtg
     7381 acactctcgt gaaccggatc gaactgaagg gcatagattt taaggaggat ggcaatatac
     7441 tgggccacaa gctcgaatac aactataatt ctcataatgt ctatatcatg gcggacaaac
     7501 agaaaaacgg catcaaggta aactttaaaa tccgacacaa tattgaggac ggctctgtcc
     7561 agcttgcgga ccattaccaa cagaacacgc cgatagggga tggtccagta cttctccctg
     7621 ataatcacta tttgtctact cagtcagctc ttagcaagga cccaaacgaa aaacgggatc
     7681 acatggtcct tcttgagttc gtcactgcgg ctgggataac actcgggatg gacgaactgt
     7741 ataaggctgc tgcatgggag ctaggaatgt ctggcggatc tgagggccag agcgacgaga
     7801 gagccctgct ggaccagctg cacaccctgc tgagcaacac cgacgccacc ggcctggagg
     7861 agatcgacag agccctgggc atccccgagc tggtgaacca gggccaggcc ctggagccca
     7921 agcaggacgg aggctgggga tctatggccg aggaattcgt gaccctgaag gacgtgggca
     7981 tggacttcac cctgggcgac tgggagcagc tgggcctgga gcagggcgac accttctggg
     8041 acaccgccct ggacaactgc caggacctgt tcctgctgga cccccccggc ggaggagacg
     8101 gccttggttt gggacacgag aagttcccca gcgacctgga cctggacatg ttcaacggca
     8161 gcctggagtg cgacatggag agcatcatca gaagcgagct gatggacgcc gacggcctgg
     8221 acttcaactt cgacagctag gccgctcgag gagggcccga acaaaaactc atctcagaag
     8281 aggatctgaa tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat
     8341 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8521 gggaggattg ggaagacaat agcaggcatg ctggggaatc tataacaaga aaatatatat
     8581 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     8641 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     8701 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     8761 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     8821 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca
     8881 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     8941 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
     9001 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
     9061 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
     9121 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
     9181 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
     9241 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
     9301 atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag
     9361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10321 acgcgcagaa aaaaaggatc tcaaga
//
