LOCUS       sequence-468456-        8076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuorescent reporter encoding H2B-GFP fusion under control of E1b 
            minimal promoter with eight ZNF35 binding sites upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EW408 (ZNF35tar-compact)x8-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..8076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   365..399
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(890..906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(890..906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(903..925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    914..930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(938..944,945..962,963..968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    983..1004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1121..1138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1292..1880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1372..1391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2051..2842,2843..2911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2674..2693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2912..3016)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3042..3497)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3179..3200)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3391..3410
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3624..3646
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3638..3655
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3639..3655
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3785..3847
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           4553..4595
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             4644..5021
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(5040..5042,5043..5753)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5781..5798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5787..5994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6027..6047)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6032..6361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6212..6347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6274..6293
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6410..7435
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6410..7435
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7495..7515)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7557..7678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7594..7613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7648..7667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat
       61 ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat atatatataa
      121 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt
      181 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa
      241 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag
      301 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag
      361 aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat
      421 catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc
      481 ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc
      541 gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg
      601 gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc
      661 agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac
      721 ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt
      781 gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg
      841 acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt
      901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1081 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2161 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     3061 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3121 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3181 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3241 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3301 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3361 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3421 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3481 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3541 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3601 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
     3661 cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt
     3721 gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctaga
     3781 ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg tgtaaaattg
     3841 acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa
     3901 gttttattat atttacactt acatactaat aataaattca acaaacaatt tatttatgtt
     3961 tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt
     4021 atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg
     4081 gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg catcccagag
     4141 ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg
     4201 aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaggcctc
     4261 cacggccaca attgacgatt gagtaacact gtatctagtc tacctacagg gctcatatat
     4321 aaagaggcct tcatatataa agagctcttc atatataaag agcagaacat atataaagag
     4381 gaggtcatat ataaagagtc gtacatatat aaagagcaga acatatataa agagaaggtc
     4441 atatataaag agcgtatcag tctagagggt atataatgga tcccatcgcg tctcagcctc
     4501 actttgagct cctccacacg aattccccga taccgtcgat tcaaggagct tgcttgttct
     4561 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaccaagct tggtaccgag
     4621 ctcggatcca actcgaggcc accatgccag agccagcgaa gtctgctccc gccccgaaaa
     4681 agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag cgcaagcgca
     4741 gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca
     4801 ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc
     4861 gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca
     4921 gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt
     4981 ccgagggtac taaggccatc accaagtaca ccagcgctaa ggatccccgg gtaccggtcg
     5041 tctcaaaggg cgaggaactt tttacgggcg ttgttccgat cctcgtcgaa ttggacgggg
     5101 acgttaatgg gcataaattc tcagttagtg gtgagggtga gggggacgcg acatatggga
     5161 aacttacgct gaagttcata tgtaccaccg gaaaattgcc ggtgccgtgg cctactctcg
     5221 tcaccaccct gacatatggt gttcagtgtt ttagccggta tccagaccat atgaaacagc
     5281 acgatttttt taaatccgct atgcctgaag gttacgtaca ggagcgaacg atatttttta
     5341 aagacgacgg aaactacaaa actagagccg aggtaaaatt cgagggtgac actctcgtga
     5401 accggatcga actgaagggc atagatttta aggaggatgg caatatactg ggccacaagc
     5461 tcgaatacaa ctataattct cataatgtct atatcatggc ggacaaacag aaaaacggca
     5521 tcaaggtaaa ctttaaaatc cgacacaata ttgaggacgg ctctgtccag cttgcggacc
     5581 attaccaaca gaacacgccg ataggggatg gtccagtact tctccctgat aatcactatt
     5641 tgtctactca gtcagctctt agcaaggacc caaacgaaaa acgggatcac atggtccttc
     5701 ttgagttcgt cactgcggct gggataacac tcgggatgga cgaactgtat aagtagctag
     5761 cgaattcgaa tttaaatcgg cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5821 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5881 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5941 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggacgcggc
     6001 cgcaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6181 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6241 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6301 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6361 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc
     6421 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     6481 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6541 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     6601 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     6661 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     6721 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     6781 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     6841 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     6901 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6961 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     7021 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     7081 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     7141 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     7201 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     7261 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     7321 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     7381 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggtcga
     7441 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7501 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcgt taactaaact
     7561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7681 atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct
     7741 tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct
     7801 tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc
     7861 cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg
     7921 cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     7981 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     8041 cgctgccggc tcggggatgc ggggggagcg ccggac
//
