LOCUS       sequence-468461-        8017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b 
            minimal promoter with four Gal4-binding sites upstream.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Wolfsberg E, Paul J-P, Tycko J, Chen B, Bassik MC, Bintu L, Alizadeh
            AA, Gao XJ
  TITLE     Machine-Guided Dual-Objective Protein Engineering for Deimmunization
            and Therapeutic Functions
  JOURNAL   bioRxiv 2025.02.24.639918
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.24.639918"
COMMENT     Sequence Label: EK0007-PB-4XnrUAS-H2B-BFP
FEATURES             Location/Qualifiers
     source          1..8017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(219..323)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(349..804)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(486..507)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     698..717
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     931..953
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     945..962
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     946..962
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1092..1154
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     5'UTR           1807..1849
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             1898..2275
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             2294..3001
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     primer_bind     complement(3029..3046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3035..3242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3275..3295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3280..3609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3460..3595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3522..3541
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3658..4683
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3658..4683
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(4743..4763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4805..4926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4842..4861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4896..4915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5689..5723
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6214..6230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6214..6230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6227..6249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6238..6254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6262..6268,6269..6286,6287..6292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6307..6328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6445..6462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6616..7204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6696..6715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7375..8017,1..149,150..218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7998..8017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
       61 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
      361 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      421 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      481 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      541 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      601 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      661 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      721 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      781 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      961 gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg
     1021 caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc
     1081 agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgtgt
     1141 aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag
     1201 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
     1261 ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
     1321 aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
     1381 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
     1441 cccagagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     1501 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     1561 aggcctccac ggccacaatt ggcaaaaagc ttaggcctcc aaggcggtgg cttctaatcc
     1621 gtgagtccta gcgggtgaca gccctccgtc ttcacaggcg gaggagagtc ttccgtaggg
     1681 ttcctcggag tactgtcctc cggcaagggt cgactctaga gggtatataa tggatcccat
     1741 cgcgtctcag cctcactttg agctcctcca cacgaattcc ccgataccgt cgattcaagg
     1801 agcttgcttg ttctttttgc agaagctcag aataaacgct caactttggc agatctacca
     1861 agcttggtac cgagctcgga tccaactcga ggccaccatg ccagagccag cgaagtctgc
     1921 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa
     1981 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca
     2041 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa
     2101 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc
     2161 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc
     2221 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc
     2281 ccgggtaccg gtcgtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct
     2341 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     2401 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     2461 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     2521 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     2581 caccacatac gaggacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     2641 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     2701 gcagaagaaa acactcggct gggaggcctt caccgaaacg ctgtaccccg ctgacggcgg
     2761 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     2821 cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     2881 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgaaacct acgtcgagca
     2941 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     3001 ttagctagcg aattcgaatt taaatcggcc tcgactgtgc cttctagttg ccagccatct
     3061 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3121 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3181 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3241 gacgcggccg caatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3301 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3361 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3421 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3481 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3541 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3601 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
     3661 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3721 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3781 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3841 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3901 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     3961 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4021 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     4081 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     4141 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     4201 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     4261 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     4321 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     4381 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     4441 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     4501 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     4561 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4621 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     4681 taggtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4741 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta
     4801 actaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg
     4981 gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     5041 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     5101 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     5161 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     5221 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     5281 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     5341 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     5401 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     5461 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     5521 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     5581 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     5641 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     5701 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     5761 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     5821 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     5881 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     5941 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     6001 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     6061 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
     6121 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     6181 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7981 cccggcgtca atacgggata ataccgcgcc acatagc
//
