LOCUS       sequence-468476-       20678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0470) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.2-Dadh-Delta-Elen0470(ElA2)
FEATURES             Location/Qualifiers
     source          1..20678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(2373..2396)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14302..14329
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14461..14488
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14920..15771)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15758..16597)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17108..18079)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19318..19405
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19747..20141
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20304..20347)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20443..20552
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccatggtt cagtgatggt gatggtgatg gtgatgctcg
     2401 agtgcggcct ccctcccttc gtaggtaggc agccattcct tcagacgggg gtcgtcggag
     2461 gtatggatga tgggcgtgcc ggtggagtcg cacggcacgg ggttgttgaa cggcgagttc
     2521 tcgggcgtgg ccttgtagat cttcacctgg taggcgcgca ggttggacgt gccggagtgc
     2581 gggtcctggg cgtagtggtc gatcagctgg ttcacctggg agaactgcca gccgtggccg
     2641 gcgtccttca cctcggggta ccaccacgtg tgctcgaggt tgatgacgtc ttccttcacg
     2701 ccgtagtaca ggtcagccac ttcgcggatc ttgccccact cggtttcgat ccacacccag
     2761 tcgccctgct cgatgccgta ctcggcggcc gtcttcgggt tgatctccac gcgcggcacg
     2821 ggccacagct cgcggcacca gggaagctgg cgatgctcgg agtggaagta caccgggatg
     2881 cgacggccgg tggtggcggt cagcgggtac tccttgatgc ggtcgccgtc cttcgggccg
     2941 tgaggcggct cggtgtaggt gggaagcctc cactcgggat ggtcgggatg gtgcgattcc
     3001 atgacggtgg accagatctc ctgcttcatc gtcggggtga accagcccgg cttccagtcg
     3061 ccgatgccct tgccggccgt gtagtcgagg cggccccaca cgcggtcgcg tgcgcgcatc
     3121 gcgccggtct ggtagcggcg gtacgtgccc cagttgcgcg gctcgatgtc cttcgcctgc
     3181 caccagccgt gctcctggaa ggccttgacg tactcgtccc acgtcttgta cttgggcgtg
     3241 acctcggcca tcggaacgcc gtggcgctcg aacgtgggct tgccgttctc gacgtgccac
     3301 gtggtgtagg tcagctcgtc gtcggtgagc agcttgatgg agtcgtccag ctgccagttg
     3361 atgtcgggcc actcgttgcc cggctcgtcg gtccacttcc agttcatgcg gcgggcgagg
     3421 tccatgacga tctcgggatc gtacttggct tccgcgggcg gctgcacgca cttgaccgtg
     3481 gcgcccatgg cgccggcgga accttggctg gcgcgcggcg agttgagctc gatccaatgg
     3541 gccaccggca ggatgatgtc ggcggtgtcg ttctgcggcg tgtgccacag gttgaggtcc
     3601 acgtagaagt caagcgagca cagggcctcc caggcgcgcg tggtgttcgt ctggctcatg
     3661 aagttgccgg actcgttcca gagggcgcgc accgggtagg ggtcgcccgt gatgacggcg
     3721 tcccacagcg aatggctgtc gcaccagtac tgccaccagc ccagaagcgg gaacttgtcg
     3781 atgccgatct gcttgcggtt gacttcctcc ggcggggtgg tggcgccggg ggcccaggcg
     3841 ctgaagccct ggaggtcgcc gtcgatgggg acgatggtcg ggccgcggtt gccgcccggg
     3901 gtgtccatgt tgccggtgat gcccacgagg ttgtcgaagg cgcggcagtt ctggatggcg
     3961 ctgcagaagt gctcgaccgc cagcatgtac tggatgccgc cgttgccgta gccggtggac
     4021 gggtcgatgc gcgtgccgta ggccgtggcc gccgcctcga tctcggaggc ggggatgccg
     4081 gtgatctcgg ccgccacctc gggcttgtac tcggcggcgc gggccttgta gtgctcccac
     4141 accggcttga ccttcacggt cttgccgtcc ttcagcgtga tctcgaactc gccttccagc
     4201 gcggggtcga tggcggggtc gaacggcatg ggatcgggca gccagccctg cgtctggccc
     4261 ggcagcaggt tgtcctgcgc ggcctcgcgg ccttggcgcg ccttctcgga gtcgtagttc
     4321 tcgccctccc agaagccgcc ggtctcgtcg atgacgcctt cctggtcggc gttgaaccag
     4381 gtgaacgcgc cgtactcgtg ctcgacgccc tcggccttca gcttctccca gttgttgtcg
     4441 tacacgagga acttataggg gctgccgccc tccttgatgt cgctttcctt gagcagcgcg
     4501 gttttcacgt cccagtagga accgtcggtg cgcacggtgg ggaagccgga gggctccatg
     4561 tcctcgcaca cgaggaacgg ggcgttcgtc cacttcttca cgtacagctc gtcgtagagc
     4621 ttcttctcga tgatgacgtt cgtccaggcc agggccaggg cgccgtcggt gccggggcgc
     4681 aggtgctgcc agtagtcggc ttccttgccc atgttggcca tgcgggggtc gacgctgatg
     4741 tgcacgtcgg cgcgcgatgc cacgtccacc gtggtgcggc aggagtcgtc gtagttggac
     4801 agctcggacg cgccgcccca ctgcacgtac actttcgggc gcgtgatggt ttccatccac
     4861 gacatggcga actgcgacac catggtggtg gcgaagtggc gcgggccctt gcagatctgc
     4921 caggcctcaa cgttgttcgg cgtttccagc atgttcttca ggatggactc cgagtgcatg
     4981 caccagatgc gcgacgtgcc cacctggcag gcgatggcct cgccgccgtg cttcgccttg
     5041 atgtccatga agttgtccac gatggactgc atggcctcgt cccacgagat gcgctgccag
     5101 ccgggctcct cgcccttcgg gttcgtgcgc ttcatgggat agtgcagacg gtcggggtgg
     5161 tacgccgcct ggatggacga ctgcgacttc gtgcagcagt tgcccatgga ctggaacgct
     5221 ccctcgtcgc cctcgacgcg tatggcccgc ccgttctgga cgatgacctt gacgccgcac
     5281 tccatcttgc cgcagccgcg gcagcacgtt ttcacggcca cggtgtcgtt gccggacacg
     5341 ctgctgtacg tctcttcggc cagcgcggtg tgcgtcgaag cgccgaacgc cgcggctgcc
     5401 gcaccggtga tggcggcggt cttgacgaac gtgcgtcgtg acatggtcag gttacccata
     5461 ctcctcctcc ttctttctcc tcgttctcgt cttgctcgtc ttcgttcgtg cgatcgggga
     5521 ggggggctgc cgggtgaagc ctcgggctcc gaaccggtgc aaagtccccg tgtcgcttgg
     5581 gccgaccata cggatgcgag ggggtgcaga gcatcgtcca aaggttggaa agcaggttcg
     5641 accgcatcat aaattcttat gatcttgtga tcatgaaaaa ttcgatagtg ctttgaactg
     5701 gggaaacttt aaccgtaggt cataaattat tctgaaacta gaatagtttc ctcaaagagg
     5761 gccgctcatc ttttccgtat aatcgaaccc gtcgacagag gggtcggcgc gagcgacgtt
     5821 atgtacgggc tcacaggggg tggggcatgg aaaagcttcg gagtgcatgg gataccgcgt
     5881 tggggacgag cctttccctc aagcgcatcg cgggcgcggc cttgcaatgg ctttggatgc
     5941 tcatgatgtt ctacagcatc gtgccgtacg gcttctcgtc ggacgtgcgc ggctcgctgt
     6001 acgtcagcct gatcatatcg ctggtggcca tggtggtgac catgctgttc gtctccgtca
     6061 tcgtgcggcg cgagcgggtg agcggcaacc gctccatcgt gctgggatcg gccatcatga
     6121 tgagcgcggg cagcgtgctc acgccgttct ccgatcctac cacgcccgtc ggcatgctgg
     6181 tgctgggcct gagcgcggtc atgaccggca cgggctcggc cgtgctgttc ttgtgctgga
     6241 tcgagctcga gtcggggctg ggaggccgct tggcgcttgt ggagcttgcc tcgtcgctgt
     6301 gcatcgcgtt cgtcgtgggg ttcttgctga tcgtagggcc ggcgataccc gtcatcgtgc
     6361 tcatcgttgt catgcccgtg ctttcggcgg cagctctcag gcgcagcgcg cccgatacgc
     6421 cgcagctgcc gcgcgagccc gagcagcctt tgtcgcgcca tacggcgacc ctgttcgcga
     6481 aggcgcttct gggcgcggtg ctcatcggca tgttgatggg gttcttcgac gtggtgtcgg
     6541 ggttcaaaac ctacgaggtg caggacatct tcggcacgta cctgtttctg gcgggcttca
     6601 tcggagcgtt ggccatctgc ctgatagccg tgttcctgca gcgcgacagc gtgttcttct
     6661 cgtaccgcgt ctccatgctg atgctgtgct tgggatgctt gtccacgccg ttcatggccg
     6721 acaacaacac gtattcgagc gcgcttatct tcggcggcta ccactgcttc gtgctggtgc
     6781 tgtgcgtggt gtgcatcgac gtggcgtcca gcttccgcgt gagcccggcg cggacgatcg
     6841 gcctggggtt cgtggcgctg tacggcggcg agaccgcagg ccagctgttc gcccattcgc
     6901 tggaggcgac cggggtcagc atgttcgacc tcacgctggt cacgctggtg gccgtgtcgc
     6961 tgctgttcat cgcgcatttg ttcctgttca cggagaccga cctcatcaaa atcggcatcg
     7021 gcgaggtcag cctgatgggc acggtgcccg aagaggccgc cgacgacccg gccgacccgg
     7081 tggatccgtg cgcgctgatc gcggagcgct tcgccctgtc gccgcgcgag accgacgtgc
     7141 ttccgctgct gctggagggc cgcaccatct cgcgcatcca ggagacgctg ttcatctcgg
     7201 cgggcacggt gagcacgcat atccgccaca tctaccagaa gacgggcgtg gacaatcgcc
     7261 aggagctcat cgacctggcc cacgagacgg ccgcgcagga cgaggcggtc tgaggcgatg
     7321 gagacggcgt atctgcacga gttcgcgaag gtggccgatt gcggcagctt cacggccgcc
     7381 gcgcgcgagc tgcatctgac gcagtcgacg ctgagcaagc acgtggcctt gttggagcgc
     7441 gaattcgggg ccgacctgtt cgtgcgcgac cgcagcggcg tgagcctgac cgaggcgggc
     7501 ggcgtgctgt acgcccaggc gcggcagatg gagaagctgc tgcgcgccac cgagctgctg
     7561 gtgcgcgcgg cgcgcgacgg gcagggttcg gcgggcgcgg gggttgcggg cgcgacggcg
     7621 ggcgcagcgg cgggcggggg tgccgtcgac ggctcgcctg cggccgcgtg cgacacggcg
     7681 cttcgctgca agtgccggct cgcggccgag cgctgcgggc tcgaccggcg cgagctgggc
     7741 gcgctcatcc tgtacgtgga ggagcgcggc ttcgaggcga tccagggcga gctggcgctg
     7801 acgcgcgacg aggtggccga cgtgctgggc agcgtgtacc gcaagctggg cgtgggcgac
     7861 aagcaggagg cgctcgattt catccattcc gtttcggaat gatggtagac gcgcccgcgg
     7921 cggttgggca tactgcgtag aagtgaaagc gctatgaagc tggggggctt cgagcaaagg
     7981 gcttcatccg aagaatggat ggagaccgaa tatgagtgct tggactgctg cgtccgtcga
     8041 agacgcggcg aacaatcttc ttgaagaact gaatagcggc gcgatgcgct tgtgcgtgga
     8101 gcatgacgcg tccgacgacg ttgcgccggc gttgctgcgc gcggctcgcg ctcgcgtcga
     8161 ggcggggtcg aacgtgcgcg tcgtgtgcgc cgacgccgtt gcagctgcgc gctttcgggc
     8221 gcttgcggcc gacgatccgc tgctgggcgc gctcgacgtg gtatcggtgc gcgagctggc
     8281 cctctcgatt ctaggcgacg cgcgggtcgg cgatgccgtg ggacgcgacg cgcgcgtgct
     8341 ggacgagaac gagctggccg tgctcatgga ggacgtgaag gtgagcggcc tcaagccggg
     8401 ccggctgcgc gagatgctca agttcttcta caagagcatc agcgactgcg ccgacgatga
     8461 cgagcgctgg ctgatcaccg ccgaagagca gaccgtgcac gccatcctca ccgagaacct
     8521 ggaggcgcgc cgcgcgttgc tgccgtgcga ggtttcgtcg ctggcgtatc gcggccttgt
     8581 gaaagccggg gtcgagcgcg agccgctgac gctggtggca gacgacttcg ggtcgctcag
     8641 caaggcgtcg cagcgcctcg tgcgccatct ggcgacggac ggcctcgtgg cggcgggccg
     8701 cacgctggcc tcgtccagct ccgaggagcc gtatccgtgc ttcgacgggt tccgcgcgct
     8761 tgccgacgag gccgactgcc tggtgacgct ggcgtgcgag cggccggccg cggcgcgcga
     8821 gtcccgcgcg ctggacgacc cggcggccga gttcgcgttc gcggcggcgt ccgtggagga
     8881 gtgcctggcc gacggcttcc ggccaggcga cgtggctgtg gccgtgccga acgccgcttg
     8941 gggcgagcgc atcgccgccg agctggaagg gcgcggcatc gccgtggagc gcgggttcga
     9001 cagcgggaag atcaagggcg acccgcgcac cgagggccgt tacgccgacc tcaagctggc
     9061 cgcgttcctg cggttgtacc tcgatccgca cgatttcacg tcgttgcgct cgtggctggg
     9121 gttcggcgat tggctgctgc gctccgatgc gtttttggag ctgatggcgt acgcgcgcga
     9181 ccatgagacg accgtgcccg aagccgtggc gcagctgcgc acgcagcgcg acgccgatcg
     9241 ctcctcgacg atattcggga agttcgacgg cccgctcgac gagctggacg agctgctgcg
     9301 cgcctgcgag gaagggctga cgcgcgaggc cgccgtagcg ctgttcgaag cgcacggaat
     9361 gccgttgtcc ccgcgccacg tggagctgct gggcgacgac cctgcgcatg cggacgtcga
     9421 gcgcttggcg cgctgcgggt tcgcgaagcc ggtggaggat gtcgcgcgcg atgccgtgca
     9481 tgtggtctcc tacgcgcgct gccacgggca ccatgtgcgc acggtgttcg tcaccggcct
     9541 ggtgaacggc ttcttgccgg cgagcgacgc cgtggacgac aagttcacca tcgaccatcg
     9601 gcgcgtggcg ctcgaacgcg agcgcgcgct gttcctggac gtgctgtcct gtgcgagcga
     9661 acgagcggta tgcacgcggt tcgtgcgcga ccttttagag aacaccgcgt tcacgaaggt
     9721 gcagacctcg cgcgtgttcg tgcgcgacgg cgagcgcttc gcgcgcgtga cgccgtcgga
     9781 gttcgcgtcg ctgaccgacg aggtgctctc tcccgcgccc gacgcgcctc gcgagctgcc
     9841 gacgaaggtg ctgtacgcgt cttcgacttt gtagctccaa cggtctgacg accgcgcgaa
     9901 ccttcgctga cttttcctga ccggtgattt tgaaatgatg aaaggactga tagatatgag
     9961 cgatgttgat gtattcgtga tcgatgacga tttcgatccc ctggccgacc tgagcctgga
    10021 cgacggcgag cgggacgatg ccgaggcgga ttacctgccg cccatccccg atgccgacaa
    10081 gagcgtggtg cctcccgtgg tgccgttgtc tgcgaccgag cgcatcgaga agctgctggc
    10141 gggcattccc ggccagcagt tccgcctgct gcacgccgtg gaattctgca ccgagccgaa
    10201 gacgatggac gaggccgtgg ccaatttgga cgcggcgtat ccgaacacga cgagcgtgta
    10261 cggctcggcg caggtggtgc agctgctcga gcgcgacggc gcgctggagc gcatcgtgga
    10321 cgaggacgcc gcccatgcgg ccgccggcga cgctgccgat acgcccgccg aggacgaggg
    10381 cttcatctcg gtgacgcccg ctccgccgtg tcactaccgt gccacgcaag cgggcctcga
    10441 tgccgtcgcg gcgcacgtca acgagggcct cgtggcggag cggatcagcg aagacgagcg
    10501 ctatctgccc atcttccagc gcgtgcttga gatgtgcgcg cgcgaaggag gctgccccac
    10561 caaggagctg gaccaggcgg tggacggcga cccgctgtgc caagagccgc gccgcttctg
    10621 cgggttcttc cgcggcaagc tggaagaaac aggcgccatc gaatggcgcg acgcctggac
    10681 cattaccgat ctggggcgca gcgtgctcgc gtccggcttg ttcgccgccg cttccccgtc
    10741 cgaacgctag agaaggagcc acccatgtcc cacgatcagg aaaccaccat cgccgacgtg
    10801 ctgcgcggcc gcatggcaac ctaccagttc ctctcgcgcc tgttccgcgt ggaggtggac
    10861 caggagctgt acgacacgct gatgtccatg cgcttcccgg cgaacaccgg caacgcgctg
    10921 gtggacgaag gctaccggat gatctgcggt tatctgagcc aagccgacgg cacggtgctc
    10981 accgagctgg cggtggacta cgtgcgcgcg ttcatcgggt cgggcaacga cggcttctcg
    11041 gccgcgtacc cgtacgagtc ggtgtacacg agcccgaagc gcctcatgat gcaggacgcc
    11101 cgcgacgagg tgctggtgct gtaccgcgcc ttcggtctgg acaagcagga aagctggaag
    11161 gagggcgagg accatatcgc gctggagctg gagttcgagc agatcctatg cgagcgcgct
    11221 atcagggcgt acgaggccgg cgacgaggac gagtgcctga agctgctgct gtcgcagcgc
    11281 aacttcctgg aggaccacct gctggcgtgg taccccatga tggcggccga cttgcagaag
    11341 ttcccgcaga ccgacttcta caagggcctg ggcaagctga ccgacggctt cctgcgcaac
    11401 gaccgcgaat ttttggacgc cgtgctgtct gagaacgaag cggattgcca tccagagcgg
    11461 aggccgcagg ccgaagccga aggatcccgt gcggcgtcgg ccgaaaccga agtcgaggtt
    11521 gcatgatgga cgagcacggc atcgagccca agcgcgatac gggcccctgc gtgtcgcgcc
    11581 gcacgttcgc cctgggagcc gtgggagcgt gcgccgtcat cgggctgggc ggcgtgaagt
    11641 acctgcccag tgccacgttg ctgcgcccgc cgggcgggca ggacgaggag cagctggcgc
    11701 gcggctgcct gcattgcgag aagtgccgcg aggtgtgccc gaagcatgcc atcgctcctg
    11761 ctcatcttga ggacggcatc ttgaacgcgc gcacaccgcg catggacttc aaaagcggct
    11821 ggtgcgattt ctgcgagaac gagccgggcg ggccgaagtg cgtcgcggcg tgcccgacgc
    11881 atgcgctttc gtgccccgac ccgtcgcagg cgatcatcgg caaggcggag atcaaccgcg
    11941 actggtgcct tgccgcgaag ggcatgggct gccacgagtg cgtcgacgtg tgcaactacg
    12001 aggcgctcga gctgggcgcc gacaacgtgc cggtggtgga cgtggatgcg tgcaacggct
    12061 gcggcgcctg cgagctggcg tgcatctcgc tgtcggcagg ttccatcacg gccggcgcca
    12121 ccgatcgcgc catcgtcgtc cgtccgaccg aagaagtgga ggcgtgatca ggatgaagaa
    12181 gctcaaaacc tatcggctgc ggttcctgat catgctggcg ctgctggccg tggtggccgt
    12241 ggggtacttc acggccggcg gcatcggcaa cttctgcggc atcggcttcg agtccatcac
    12301 gctgctgtgc cccctaggcg cgctgctggc catgatcgcc gagcgcacag ccatcccgat
    12361 ggccgtgatc agcgttgcgg tcgtgctgct ggtgtgtatc gtgctaggca aggtgttctg
    12421 cgcctgggcg tgccccgtgc acttcctgtc gcgaggaagg aagaaggggg ataaggggga
    12481 caaggagggt aaagggggca aggttgcttc cccttgctcc gcgtgcgctt ccccttgcgg
    12541 caaagccaag ggcatcaaga tcgacagccg tcacggcatc ctcgctgcgg cgttgggctc
    12601 cacgctcgtc ttcggcttcc cggtgttctg cctcgtctgc ccgatcggcc tcacgttcgc
    12661 cacggtgctg ctggtcatgc gcctgttcgc gttcggcgac gccacgtgga cggtcgtcgc
    12721 cttcccggcc atcatcgcgc tggagatcct gctgctgccg aagtggtgcc agcgcttctg
    12781 cccgttgggc gcgttgcttt cgctgttcag cgggctgaac cggacgttcc gcccccaggt
    12841 ggacgccgag aagtgcctgc gcgagggtcg cggcaaggcc tgcaacctgt gcgagcaggc
    12901 atgccccgag ggcatcaacc tgcacgatgt cgcggcgggc gagaccacgc tcaacgactg
    12961 cagcaagtgc cgcgcgtgcg ccgacgtgtg ccccgagcgc gccatcacgt tcccgttgct
    13021 gccgaagaag caggcgcagg cgacgccgct gcccgtcgac gacgagatcc cggaaagggg
    13081 ataggccatg tccctgatca acggcctgct ggccctgttg ggcgagatcg agagcgatgc
    13141 catcgccgtg catgaggaac gctgcatctc ggttcgcaac cgcaacgccg actgcctgcg
    13201 ctgcgtggag gcatgcacgt cgggcgcgct ggcgtaccgc gcgggcgagc tgctcgtaga
    13261 gcccgagcgc tgcatcggat gcggcacgtg cgccacggcg tgccccacct gcgccatcga
    13321 gctgcgcaac ccgacggatg ccgagctgac ggcgcagctc aagcactcca tcgtggcgac
    13381 gaaaggccat ccggtcatcg tgtgcgagca ggcgctcgag gcggccaacg tcgcggccga
    13441 cgatgcgtcg gccgcgtgcg cggtgccgtg cctggggcgc atggacgagt cggcgctcgt
    13501 ggggttggcg gcgtaccgcg ccttcgacgc gacgctggcc tgcggttcgt gcgagacgtg
    13561 cccacatgct ccgggcggcg cgctcgtgcg cgaggtggtg gagggcgcgc gcaacctgct
    13621 tgaggcgttc ggatcctcca tgcccatcga cctggaggaa tgcgtgcccg agcgcgtgtt
    13681 ggagcccggc gggtcgcacg gccaggcagg cagcgacggg gtggggcggc gcgacttctt
    13741 ccgctcggcc aaggacgcat cgacccgcgc agctggcgcg gcggtggccg aggagttggg
    13801 gatggtcgag gccgagcccg ttcctgcggc gcatcgcaaa gtgggcgcag atgggacgct
    13861 gtcgcagttc gtccccacgc gccgcgtgcg gctgtacaac tacctgcagc acgtggggca
    13921 accgacggcc gacgaggtgg agacgcgtgt catcggcgcc gtgtccatcg atgcccagaa
    13981 gtgcagttcg tgccgcatgt gcgcggtgtt ctgtcccacg ggcgccatca agaagctcga
    14041 cgagggcggc gtgttcggca tcgtgcaccg tccgagcgcc tgcatgcagt gccgcctgtg
    14101 cgagcacatc tgtcccgagc aggccatcgc ggtgagcggg cgcgtgccca tcaagcagtt
    14161 catgggcaag gaagccgtgt gcttcgctat gaagcgtcca acctggaccc cgaacaagcc
    14221 ctcatcgatg tacgataagc tgcacgcgac gctcggcgaa cacttggaga tgtgcatgtt
    14281 ctagactagt cgactcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
    14341 gagagagaat ataaaaagcc agattattaa tccggctttt ttattattta attcctgtta
    14401 taaaaaaagg atcaattttg aactctctcc caaagttgat cccttaacga tccctgcagg
    14461 agaaggccat cctgacggat ggcctttttg aagctttcta gaatcggggg cccccggggg
    14521 gattctagaa caatggaagt cctcgtctcg ccctcgaatt agcccgctta atgagcgggc
    14581 tttttttgaa ttaattctcg cgagctggca cgacaggttt cccgactgga aagcgggcag
    14641 tgagcgcaac gcaattaatg taagttagcg cgaattgcaa gctggccgac gcgctgggct
    14701 acgtcttgct ggcgttcggg agcagaagag catacatctg gaagcaaagc caggaaagcg
    14761 gcctatggag ctgtgcggca gcgctcagta ggcaattttt caaaatattg ttaagccttt
    14821 tctgagcatg gtatttttca tggtattacc aattagcagg aaaataagcc attgaatata
    14881 aaagataaaa atgtcttgtt tacaatagag tggggggggt cagcctgccg ccttgggccg
    14941 ggtgatgtcg tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc
    15001 cggcaacgcc tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac tggcctctga
    15061 cggccagaca tagccgcaca aggtatctat ggaagccttg ccggttttgc cggggtcgat
    15121 ccagccacac agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc
    15181 gtccatgctg atgcgcacat gctggccgcc acccatgacg gcctgcgcga tcaaggggtt
    15241 cagggccacg tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa
    15301 cccctgccgc ttgcggccat tctgggcgat gatggatacc ttccaaaggc gctcgatgca
    15361 gtcctgtatg tgcttgagcg ccccaccact atcgacctct gccccgattt cctttgccag
    15421 cgcccgatag ctacctttga ccaccatggc atcagcggtg acggcctccc acttgggttc
    15481 caggaacagc cggagctgcc gtccgccttc ggtcttgggt tccgggccaa gcactaggcc
    15541 attaggccca gccatggcca ccagcccttg caggatgcgc agatcatcag cgcccagcgg
    15601 ctccgggccg ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt
    15661 gctgcgcttg cgctcgcccc gcttgagggc acggaacagg ccgggggcca gacagtgcgc
    15721 cgggtcgtgt cggacgtggc tgaggctgtg cttgttctta ggcttcacca cggggcaccc
    15781 ccttgctctt gcgctgcctc tccagcacgg cgggcttgag caccccgccg tcatgccgcc
    15841 tgaaccaccg atcagcgaac ggtgcgccat agttggcctt gctcacaccg aagcggacga
    15901 agaaccggcg ctggtcgtcg tccacacccc attcctcggc ctcggcgctg gtcatgctcg
    15961 acaggtagga ctgccagcgg atgttatcga ccagtaccga gctgccccgg ctggcctgct
    16021 gctggtcgcc tgcgcccatc atggccgcgc ccttgctggc atggtgcagg aacacgatag
    16081 agcacccggt atcggcggcg atggcctcca tgcgaccgat gacctgggcc atggggccgc
    16141 tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc
    16201 cctcggcggc gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc
    16261 cgatcagcgg ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt
    16321 gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt
    16381 agatcaccgg gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct
    16441 gtgcggccag ttgcagggcc agcatggatt taccggcacc accgggcgac accagcgccc
    16501 cgaccgtacc ggccaccatg ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga
    16561 acgcctccag aatattgata ggcttatggg tagccattga ttgcctcctt tgcaggcagt
    16621 tggtggttag gcgctggcgg ggtcactacc cccgccctgc gccgctctga gttcttccag
    16681 gcactcgcgc agcgcctcgt attcgtcgtc ggtcagccag aacttgcgct gacgcatccc
    16741 tttggccttc atgcgctcgg catatcgcgc ttggcgtaca gcgtcagggc tggccagcag
    16801 gtcgccggtc tgcttgtcct tttggtcttt catatcagtc accgagaaac ttgccggggc
    16861 cgaaaggctt gtcttcgcgg aacaaggaca aggtgcagcc gtcaaggtta aggctggcca
    16921 tatcagcgac tgaaaagcgg ccagcctcgg ccttgtttga cgtataacca aagccaccgg
    16981 gcaaccaata gcccttgtca cttttgatca ggtagaccga ccctgaagcg cttttttcgt
    17041 attccataaa acccccttct gtgcgtgagt actcatagta taacaggcgt gagtaccaac
    17101 gcaagcacta catgctgaaa tctggcccgc ccctgtccat gcctcgctgg cggggtgccg
    17161 gtgcccgtgc cagctcggcc cgcgcaagct ggacgctggg cagacccatg accttgctga
    17221 cggtgcgctc gatgtaatcc gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg
    17281 cctcggccat ggccttgccg atttcctcgg cactgcggcc ccggctggcc agcttctgcg
    17341 cggcgataaa gtcgcacttg ctgaggtcat caccgaagcg cttgaccagc ccggccatct
    17401 cgctgcggta ctcgtccagc gccgtgcgcc ggtggcggct aagctgccgc tcggacagtt
    17461 cgaggctggc cagcctgcgg gccttctcct gctgccgctg ggcctgctcg atctgctggc
    17521 cagcctgctg caccagcgcc gggccagcgg tggcggtctt gcccttggat tcacgcagca
    17581 gcacccacgg ctgataaccg gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg
    17641 ccaagcggcc atagtggcgg ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca
    17701 gcgtccgggc aatctgcccc cgaagttcac cgcctgcggc gtcggccacc ttgacccatg
    17761 cctgatagtt cttcgggctg gtttccacta ccagggcagg ctcccggccc tcggctttca
    17821 tgtcatccag gtcaaactcg ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct
    17881 cggcgggcct gatatacacg tcattgccct gggcattcat ccgcttgagc catggcgtgt
    17941 tctggagcac ttcggcggct gaccattccc ggttcatcat ctggccggtg gtggcgtccc
    18001 tgacgccgat atcgaagcgc tcacagccca tggccttgag ctgtcggcct atggcctgca
    18061 aagtcctgtc gttcttcatc gggccaccaa gcgcagccag atcgagccgt cctcggttgt
    18121 cagtggcgtc aggtcgagca agagcaacga tgcgatcagc agcaccaccg taggcatcat
    18181 ggaagccagc atcacggtta gccatagctt ccagtgccac ccccgcgacg cgctccgggc
    18241 gctctgcgcg gcgctgctca cctcggcggc tacctcccgc aactctttgg ccagctccac
    18301 ccatgccgcc cctgtctggc gctgggcttt cagccactcc gccgcctgcg cctcgctggc
    18361 ctgctgggtc tggctcatga cctgccgggc ttcgtcggcc agtgtcgcca tgctctgggc
    18421 cagcggttcg atctgctccg ctaactcgtt gatgcctctg gatttcttca ctctgtcgat
    18481 tgcgttcatg gtctattgcc tcccggtatt cctgtaagtc gatgatctgg gcgttggcgg
    18541 tgtcgatgtt cagggccacg tctgcccggt cggtgcggat gccccggcct tccatctcca
    18601 ccacgttcgg ccccaggtga acaccgggca ggcgctcgat gccctgcgcc tcaagtgttc
    18661 tgtggtcaat gcgggcgtcg tggcctgccc gctctaatgc ccggttggca tggtcggccc
    18721 atgcctcgcg ggtctgctca agccatgcct tgggcttgag cgcttcggtc ttctgtgccc
    18781 cgcccttctc cggggtcttg ccgttgtacc gcttgaacca ctgagcggcg ggccgctcga
    18841 tgccgtcatt gatccgctcg gagatcatca ggtggcagtg cgggttctcg ccgccaccgg
    18901 catggatggc cagcgtatac ggcaggcgct cggcaccggt caggtgctgg gcgaactcgg
    18961 acgccagcgc cttctgctgg tcgagggtca gctcgaccgg cagggcaaat tcgacctcct
    19021 tgaacagccg cccattggcg cgttcataca ggtcggcagc atcccagtag tcggcgggcc
    19081 gctcgacgaa ctccggcatg tgcccggatt cggcgtgcaa gacttcatcc atgtcgcggg
    19141 catacttgcc ttcgcgctgg atgaagtcgg ccttggccct ggccgattgg ccgcccgacc
    19201 tgctgccggt tttcgccgta aggtgataaa tcgccatgct gcctcgctgt tgcttttgct
    19261 tttcggctcc atgcaatggc cctcggagag cgcaccgccc gaagggtggc cgttaggcca
    19321 gtttctcgaa gagaaaccgg taagtgcgcc ctcccctaca aagtagggtc gggattgccg
    19381 ccgctgtgcc tccatgatag cctacgagac agcacattaa caatggggtg tcaagatggt
    19441 taaggggagc aacaaggcgg cggatcggct ggccaagctc gaagaacaac gagcgcgaat
    19501 caatgccgaa attcagcggg tgcgggcaag ggaacagcag caagagcgca agaacgaaac
    19561 aaggcgcaag gtgctggtgg gggccatgat tttggccaag gtgaacagca gcgagtggcc
    19621 ggaggatcgg ctcatggcgg caatggatgc gtaccttgaa cgcgaccacg accgcgcctt
    19681 gttcggtctg ccgccacgcc agaaggatga gccgggctga atgatcgacc gagacaggcc
    19741 ctgcggggct gcacacgcgc ccccaccctt cgggtagggg gaaaggccgc taaagcggct
    19801 aaaagcgctc cagcgtattt ctgcggggtt tggtgtgggg tttagcgggc tttgcccgcc
    19861 tttccccctg ccgcgcagcg gtggggcggt gtgtagccta gcgcagcgaa tagaccagct
    19921 atccggcctc tggccgggca tattgggcaa gggcagcagc gccccacaag ggcgctgata
    19981 accgcgccta gtggattatt cttagataat catggatgga tttttccaac accccgccag
    20041 cccccgcccc tgctgggttt gcaggtttgg gggcgtgaca gttattgcag gggttcgtga
    20101 cagttattgc aggggggcgt gacagttatt gcaggggttc gtgacagtta gtacgggagt
    20161 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
    20221 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
    20281 atgtggcggc cctcgcgaga attaattcag ataaaaaaaa tccttagctt tcgctaagga
    20341 tgatttctag cgatgaccct gctgattggt tcgctgaccg tagactggtt ggcttggttt
    20401 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
    20461 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
    20521 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
    20581 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gacatcgcgc gcgcgcgcgc
    20641 ggatgctagc ggcgcgccaa aaaaaagccc gctcatta
//
