LOCUS       sequence-468483-       10189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pMYB60:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    57..81
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      169..879
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1174..1965
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2306..2894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2795..2814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3230..4378
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4524..4548
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5050..5395
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5338..5358
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5486..6280
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5540..5559)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6150..6169
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6293..6545
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7872..7896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9928..10180
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 agaggatgca catgtgaccg agggacacga agtgatccgt ttaaactatc agtgtttgac
       61 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
      121 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac
      181 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
      241 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct
      301 cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga
      361 ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag
      421 ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat
      481 gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga
      541 cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca
      601 cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg
      661 cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa
      721 ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc
      781 ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc
      841 gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgaggca
      901 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
      961 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1021 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     1081 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     1141 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     1201 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     1261 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
     1321 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     1381 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     1441 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     1501 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     1561 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     1621 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     1681 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     1741 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     1801 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     1861 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1921 gtccattacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     1981 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     2041 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     2101 taataagccc tacacaaatt gggagatata tcatgctgtc agaccaagtt tactcatata
     2161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggca gatcctagat
     2941 gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca tcaagtgttt
     3001 tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg tattcgtgca
     3061 gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt
     3121 cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata
     3181 agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac
     3241 gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc
     3301 cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg
     3361 ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc
     3421 tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc
     3481 aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa
     3541 aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa
     3601 acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc
     3661 ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa
     3721 caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt
     3781 gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca
     3841 ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga
     3901 gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg
     3961 cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga
     4021 atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg
     4081 ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt
     4141 catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag
     4201 ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga
     4261 ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg
     4321 cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg
     4381 ccttgtgggg tcagttccgg ctgggggttc agcagcccct gctcggatct gttggaccgg
     4441 acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     4501 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     4561 agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac actctgtggg
     4621 tctcatgccg aattcggatc cggaggtcaa catggtggag cacgacactc tggtctactc
     4681 caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag
     4741 gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
     4801 gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat
     4861 cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     4921 cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgataacat
     4981 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     5041 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     5101 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     5161 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     5221 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     5281 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     5341 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     5401 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     5461 aattacattt acaattatcg atacaatgat tgaacaagat ggattgcacg caggttctcc
     5521 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5581 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5641 cctgtccggt gccctgaatg aactccaaga cgaggcagcg cggctatcgt ggctggccac
     5701 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5761 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5821 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5881 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5941 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6001 caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg gcgatgcctg
     6061 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6121 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6181 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6241 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcttgtcaag cagatcgttc
     6301 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     6361 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     6421 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     6481 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     6541 agatcgacgc tgcaagaatt caagcttgga gcacaaggac acaaggacat atggtatgat
     6601 gatatgcttt gtttctctgc ttctcttact aatttgaagc tgttggattg atttgtctct
     6661 tcttacgtcc ccttcttttt tttttcgttt tcttttgtcg catagaccag gcaggggcta
     6721 gggcctagtg atgggtattg gcccaatact attgggttat ttgcctggtt tattatttcg
     6781 attttaggtt aattcaattt taagaatacg tagatttgtt tggtttagtt tggtttggtt
     6841 gcactaagtt cggttttaca taaatagaat ctaacactac taattgttat acgtaaaata
     6901 caacaacaat aacagatttt tcgtttcaat tttcgtttaa gagggtagac attttggttt
     6961 ggtttggttc attttttttt tccctttcaa attcacatcc ttcacgtaga tgacaaaata
     7021 aagaaaaaca tgaatgaaag ttgtaacttg taagcatcaa catggaaatc atatcacaaa
     7081 gaacacaaat ctaactaatg ggccttttca catattggta taattataag ctgtaagaat
     7141 attagttaaa cagaggcaac gagagatgcg tgatatatga aaagttgaaa acaaaagaca
     7201 tggatctaaa gagtcaagca aaatgtaata tctttttttc ttctaaactt gaggatgtcc
     7261 aagttgcagt gaatgattcc ctttaatcat ggagaaattc aatgaaataa ttgtgtttct
     7321 tcccacactt tatctttatt tattttctta ccacaattac aactattatc acaaaaatga
     7381 aagtaacata gcttgtgact cttcttccat ttatgagttg attatcacta tatttataag
     7441 taattaccaa cgaatgttcc aaattaagca aaatattgta atcgatacac tatgtattca
     7501 tctacaatat gttaacgagc tccttttatg gaaatatttc gattgaaaaa acatttgatg
     7561 gatcgttcac taaataaata atccagtaac gttttcttaa gggagatata catattcgtg
     7621 tggagatcaa catatcttcg ttaattgact acgcaaaata gttaatggaa aaggcagagt
     7681 gactcgtgag cttggcagat ccaaaagagg ttgtcaagaa aaagcagatt taaaagttct
     7741 tccctcttct ttaagtcacc cattaatttc acatatatgt acatacatgt tgcatttaac
     7801 tcatatacat acatattctc acatctataa agagagcata agactcagag agatctagaa
     7861 ctagtgatat cacaagtttg tacaaaaaag caggcttaaa tgggtcagtc ccttatgtta
     7921 cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt
     7981 ctggatcgcg aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc
     8041 cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat
     8101 tatgcgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggtaa gtcttactct
     8161 ctcttttttg gtctgtattt ttaatttttt gaagtatact atttgtactg acgctaataa
     8221 tcttttttca ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
     8281 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
     8341 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
     8401 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
     8461 caagaaaaag cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt
     8521 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
     8581 gcaagactgt aaccacgcgt ctgttgactg gcaggtactt catgcttcaa cgtgtaactt
     8641 aagagatact gtgtgaaatt ttatatttcc atacatttgc ttgacctttg ctttttgtca
     8701 atttttttcc ccttacaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg
     8761 gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg
     8821 cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag
     8881 acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa
     8941 cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg
     9001 gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac
     9061 tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac
     9121 tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc
     9181 tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca
     9241 gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat agcgcgtgac
     9301 aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa
     9361 ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt
     9421 ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc
     9481 tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa
     9541 acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg
     9601 attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac
     9661 atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc
     9721 gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc
     9781 atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg
     9841 gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag
     9901 ggaggcaaac aatgagcttg tcaagcagat cgttcaaaca tttggcaata aagtttctta
     9961 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    10021 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    10081 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    10141 gataaattat cgcgcgcggt gtcatctatg ttactagatc gacgctact
//
