LOCUS       sequence-468486-       10521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pCSRM2C:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    72..96
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      184..894
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1189..1980
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2321..2909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2810..2829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3245..4393
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4539..4563
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5065..5410
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5353..5373
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5501..6295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5555..5574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6165..6184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6308..6560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      join(10275..10521,1..6)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 tagatcgacg ctactagagg atgcacatgt gaccgaggga cacgaagtga tccgtttaaa
       61 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
      121 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc
      181 agccgccttt gcgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
      241 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg
      301 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
      361 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
      421 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
      481 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
      541 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
      601 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
      661 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
      721 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
      781 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat
      841 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg
      901 ccaattcccg aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
      961 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     1021 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     1081 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1141 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     1201 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1261 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1321 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1381 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1441 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     1501 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     1561 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     1621 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     1681 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     1741 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     1801 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1861 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     1921 cagttggaag aatttgtcca ttacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     1981 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     2041 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     2101 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg ctgtcagacc
     2161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2941 tggcagatcc tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt
     3001 ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc
     3061 gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt
     3121 ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg
     3181 gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg
     3241 gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt
     3301 ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac
     3361 cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt
     3421 gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct
     3481 tgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac
     3541 gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca
     3601 ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga
     3661 tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct
     3721 gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca
     3781 cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga
     3841 actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac
     3901 cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac
     3961 gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg
     4021 cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa
     4081 gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga
     4141 ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat
     4201 gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct
     4261 catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg
     4321 cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca
     4381 ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag cccctgctcg
     4441 gatctgttgg accggacagt agtcatggtt gatgggctgc ctgtatcgag tggtgatttt
     4501 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
     4561 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt
     4621 tgaacactct gtgggtctca tgccgaattc ggatccggag gtcaacatgg tggagcacga
     4681 cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga
     4741 gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
     4801 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
     4861 taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc
     4921 acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga
     4981 ttgatgtgat aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     5041 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     5101 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     5161 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     5221 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
     5281 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     5341 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     5401 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
     5461 aacaaacaac attacaatta catttacaat tatcgataca atgattgaac aagatggatt
     5521 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5581 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5641 ttttgtcaag accgacctgt ccggtgccct gaatgaactc caagacgagg cagcgcggct
     5701 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5761 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5821 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5881 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5941 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     6001 agccgaactg ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac
     6061 ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6121 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6181 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6241 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcttg
     6301 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     6361 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     6421 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     6481 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     6541 gtcatctatg ttactagatc gacgctgcaa gaattcaagc ttggagcttt ccctctcgta
     6601 aaccgcaatc gtttcatctt ccctttaatt ttgtttgttt gcatgctagc tcattttgcg
     6661 tttcttcttc cgaatagagc cgtcagcaca caataaaata agctattcta ggaccacaaa
     6721 gcattgcata caaacaaaac tggaacaaac aaaattagta atccttttcg caaaagaaag
     6781 atacactaca aagcaaacaa aagctcgact gtttcccaat gaggcgtgca tgcacagaga
     6841 tgtaattcca tggaagcaaa cagacaatat ggcacacagg attcgcatgc ttttcgtgtc
     6901 cgttcactga aattttctct tggtcttttc cattgttcaa gtacgtgtgc ctactcactg
     6961 actggcaaat actagatcag ttctccaaac gaaattgtaa tccatggatc tatctactct
     7021 tgtgcatcag tggatgcaga gggccgtggg gttatctttc tttttcgacg aaaaaaagct
     7081 actccagcct ccggctaata tggtcgaaaa ttccgtactc acatttaccc tcacaaacaa
     7141 actttctctc tcgtaaaccg caatcgtttc atcttccctt taatttgttt gtttgcatgc
     7201 cagctcattt tgagtttctt cttcggaata gggccgtgca acacacaata aaataagcta
     7261 ctctaggacc acaaagcatt gcatacaaac aaagccggac cagaaaattt aatatttttt
     7321 gcaaaagaaa gatactagaa agcaaacaga aactctactg ttcctaatgg aggcgtgctt
     7381 agatctactt cctccgattc taaattgttg tcgaaatatt acatgtatct aaacgctttt
     7441 taagaataaa tgcatccata tttgagcaaa tttgagtcaa gaatttagag tcagagaaag
     7501 tatatatgta aatccatgga agcaaacaga caacatggca catagggttc gcatgctttt
     7561 cgtgtacgtt cacctgaaat tttctcttgg tcttttccat gttcacgtaa gtgtgcctag
     7621 tcactggcaa atatagatca gttttatcca cacgaaattg taatccatgg atctactcca
     7681 gctaatatag ggtcgaaact cggtgctcac atttacacgc aaattaatga gtgtattaac
     7741 ttgaaaaaaa ctcggtgctc acgtttacac gcaaattaat gagtgtatta acttgagttt
     7801 gatgcaagtt taataatgtc catggaatta tccgtgatat gtctttcact aggtggagtg
     7861 tgattctaag cgtgtggtgt ttctatttgc ttcctccgtt tcataattct tattaaaatc
     7921 ttacatgtgt ctagatgttt tttaaaaata aatacattca ttttttaagt aaatttgaga
     7981 caaaaattat gaaacagaca aaatactctg caatggccaa aagtaaaagt aaaaaggaaa
     8041 ataaatacga agatgtttgt atgtagcaca gctagcaacc cagccagcta gggacggacg
     8101 gtaccactga ctggcctccg gcgtcctata agaagcaaag ggaagccagc ttagccgtcc
     8161 cgtctcaaat cccaacaagg gttgttcgag ctgcagagga gagaggaagg agaggataag
     8221 tgttagcgag cgagagctcg agggcaatgg gtcagtccct tatgttacgt cctgtagaaa
     8281 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     8341 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
     8401 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
     8461 tctggtatca gcgcgaagtc tttataccga aaggtaagtc ttactctctc ttttttggtc
     8521 tgtattttta attttttgaa gtatactatt tgtactgacg ctaataatct tttttcaggt
     8581 tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg
     8641 gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc
     8701 acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg
     8761 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag
     8821 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc
     8881 acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac
     8941 cacgcgtctg ttgactggca ggtacttcat gcttcaacgt gtaacttaag agatactgtg
     9001 tgaaatttta tatttccata catttgcttg acctttgctt tttgtcaatt tttttcccct
     9061 tacaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
     9121 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
     9181 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
     9241 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
     9301 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
     9361 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
     9421 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
     9481 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
     9541 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
     9601 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
     9661 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
     9721 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
     9781 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
     9841 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
     9901 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
     9961 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
    10021 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
    10081 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
    10141 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
    10201 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat
    10261 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    10321 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    10381 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    10441 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    10501 gcgcggtgtc atctatgtta c
//
