LOCUS       sequence-468487-       12521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for integration at the native URA3 locus with 3xmCherry 
            under control of the SpH2B promoter and 3xGFP under control of the 
            ScACT1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Colarusso AV, Williams AM, Gladfelter AS, Wirshing ACE, Lew DJ
  TITLE     Optimized vectors for genetic engineering of Aureobasidium 
            pullulans.
  JOURNAL   Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi: 10.1091/mbc.E25-02-0059. 
            Epub 2025 Apr 9.
  PUBMED    40202829
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2025-06-1"; volume: "36(6):mr5. doi"; pages: " 
            10.1091/mbc.E25-02-0059. Epub 2025 Apr 9"
COMMENT     Sequence Label: pAP-U2-2
FEATURES             Location/Qualifiers
     source          1..12521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..150
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      5471..5658
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5526..5550)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7024..7044)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7024..7042)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7063..7079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7063..7079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7076..7098)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7087..7103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7111..7117,7118..7135,7136..7141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7156..7177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7294..7311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7465..8053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7545..7564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8224..9015,9016..9084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8847..8866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9085..9189)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9216..9672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9303..9322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9513..9534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9798..9820
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9812..9829
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9813..9829
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9839..9858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9839..9857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      12289..12486
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aaaaaaaaga aaaatttgaa atataaataa cgttcttaat actaacataa ctattaaaaa
       61 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
      121 gtgggaggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc gacaaaggaa
      181 aaggggcctg attgttaact cttgtagagc tcgtccatgc cgccggtaga gtggcggccc
      241 tcggcgcgct cgtattgctc gacgatggtg tagtcctcgt tgtgagaggt gatgtcgagc
      301 ttgatgttga cgttgtaggc gccagggagt tggacaggct tcttggcctt gtaggtggtc
      361 ttgacctcgg cgtcgtagtg gccgccgtcc ttgagcttga ggcgttgctt gatctcgccc
      421 ttgagggcgc cgtcctcagg gtacatgcgc tcagaagagg cctcccagcc catggtcttc
      481 ttttgcatga cagggccgtc agaagggaag ttggtgccgc ggagcttgac cttgtagatg
      541 aactcgccgt cttggagaga agagtcttgg gtgacggtga cgacgccgcc gtcctcgaag
      601 ttcatgacgc gctcccactt gaagccctca gggaaagaga gcttgaggta gtcagggatg
      661 tcggcagggt gcttgacgta ggccttagag ccgtacatga attgaggaga gaggatgtcc
      721 caggcgaaag ggagagggcc gcccttggtg accttgagct tggcggtttg ggtgccctcg
      781 taagggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttgac agagccctcc
      841 atgtggacct tgaagcgcat gaactccttg atgatggcca tgttgtcctc ctcgccctta
      901 gagaccatgc cagcagagcc agcagacttg tagagctcgt ccatgccgcc ggtagagtgg
      961 cggccctcgg cgcgctcgta ttgctcgacg atggtgtagt cctcgttgtg agaggtgatg
     1021 tcgagcttga tgttgacgtt gtaggcgcca gggagttgga caggcttctt ggccttgtag
     1081 gtggtcttga cctcggcgtc gtagtggccg ccgtccttga gcttgaggcg ttgcttgatc
     1141 tcgcccttga gggcgccgtc ctcagggtac atgcgctcag aagaggcctc ccagcccatg
     1201 gtcttctttt gcatgacagg gccgtcagaa gggaagttgg tgccgcggag cttgaccttg
     1261 tagatgaact cgccgtcttg gagagaagag tcttgggtga cggtgacgac gccgccgtcc
     1321 tcgaagttca tgacgcgctc ccacttgaag ccctcaggga aagagagctt gaggtagtca
     1381 gggatgtcgg cagggtgctt gacgtaggcc ttagagccgt acatgaattg aggagagagg
     1441 atgtcccagg cgaaagggag agggccgccc ttggtgacct tgagcttggc ggtttgggtg
     1501 ccctcgtaag ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttgacagag
     1561 ccctccatgt ggaccttgaa gcgcatgaac tccttgatga tggccatgtt gtcctcctcg
     1621 cccttagaga ccatgccaga gccgccagag cccttgtaga gctcgtccat gccgccggta
     1681 gagtggcggc cctcggcgcg ctcgtattgc tcgacgatgg tgtagtcctc gttgtgagag
     1741 gtgatgtcga gcttgatgtt gacgttgtag gcgccaggga gttggacagg cttcttggcc
     1801 ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttgagctt gaggcgttgc
     1861 ttgatctcgc ccttgagggc gccgtcctca gggtacatgc gctcagaaga ggcctcccag
     1921 cccatggtct tcttttgcat gacagggccg tcagaaggga agttggtgcc gcggagcttg
     1981 accttgtaga tgaactcgcc gtcttggaga gaagagtctt gggtgacggt gacgacgccg
     2041 ccgtcctcga agttcatgac gcgctcccac ttgaagccct cagggaaaga gagcttgagg
     2101 tagtcaggga tgtcggcagg gtgcttgacg taggccttag agccgtacat gaattgagga
     2161 gagaggatgt cccaggcgaa agggagaggg ccgcccttgg tgaccttgag cttggcggtt
     2221 tgggtgccct cgtaagggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttg
     2281 acagagccct ccatgtggac cttgaagcgc atgaactcct tgatgatggc catgttgtcc
     2341 tcctcgccct tagagaccat gtttaattaa ttgtgtgtgt gatggatgag agtggtgcgg
     2401 gtgaaaggtc ttggtcaagt cgcaaagtgt ttgtgaaaaa gacgtagcgg gggaagcgca
     2461 cacacgcccg ccttttatag acgaagcttc gagcaggtca tttaacgatg cttagtagca
     2521 atatacaata aagcctaatt gggactaatt acggtcacgt gaaaaactca ccatgtagcc
     2581 ttttatggaa atattgtaca cgctactaag ccactgtaat atcggcctca tcgtctccgc
     2641 ttggagcata aaacccagcg ctgggaacat atatacacaa ttacagtaac aataacaaga
     2701 ggacagatac taccaaaatg tgtggggaag cgggtaagct gccacagcaa ttaatgcaca
     2761 acatttaacc tacattcttc cttatcggat cttcaaaacc cttaaaaaca tatgcctcac
     2821 cctaacatat tttccaatta accctcaata tttctctgtc acccggcctc tattttccat
     2881 tttcttcttt acccgccacg cgtttttttc tttcaaattt ttttcttcct tcttcttttt
     2941 cttccacgtc ctcttgcata aataaataaa ccgttttgaa accaaactcg cctctctctc
     3001 tcctttttga aatatttttg ggtttgtttg atcctttcct tcccaatctc tcttgtttaa
     3061 tatatattca tttatatcac gctctctttt tatcttcctt tttttcctct ctcttgtatt
     3121 cttccttccc ctttctactc aaaccaagaa gaaaaagaaa aggtcaatct ttgttaaaga
     3181 ataggatctt ctactacatc agcttttaga tttttcacgc ttactgcttt tttcttccca
     3241 agatcgaaaa tttactgaat taacaaaacc ttaagatgtc taagggcgag gagctcttca
     3301 ccggcgtcgt ccctatcctc gtcgagctcg acggcgacgt caacggccac aagttctctg
     3361 tctctggcga gggcgagggc gacgccacct acggcaagct caccctcaag ttcatctgca
     3421 ccaccggcaa gctccctgtc ccttggccta ccctcgtcac caccttcggc tacggcgtcc
     3481 aatgcttcgc ccgctaccct gaccacatga agcaacacga cttcttcaag tctgccatgc
     3541 ctgagggcta cgtccaagag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3601 gcgccgaggt caagttcgag ggcgacaccc tcgtcaaccg catcgagctc aagggcatcg
     3661 acttcaagga ggacggcaac atcctcggcc acaagctcga gtacaactac aactctcaca
     3721 acgtctacat catggccgac aagcaaaaga acggcatcaa ggtcaacttc aagatccgcc
     3781 acaacatcga ggacggctct gtccaactcg ccgaccacta ccaacaaaac acccctatcg
     3841 gcgacggccc tgtcctcctc cctgacaacc actacctctc tacccaatct gccctctcta
     3901 aggaccctaa cgagaagcgc gaccacatgg tcctcctcga gttcgtcacc gccgccggca
     3961 tcacccacgg catggacgag ctctacaagg gctctggcgg ctctggcatg tctaagggcg
     4021 aggagctctt caccggcgtc gtccctatcc tcgtcgagct cgacggcgac gtcaacggcc
     4081 acaagttctc tgtctctggc gagggcgagg gcgacgccac ctacggcaag ctcaccctca
     4141 agttcatctg caccaccggc aagctccctg tcccttggcc taccctcgtc accaccttcg
     4201 gctacggcgt ccaatgcttc gcccgctacc ctgaccacat gaagcaacac gacttcttca
     4261 agtctgccat gcctgagggc tacgtccaag agcgcaccat cttcttcaag gacgacggca
     4321 actacaagac ccgcgccgag gtcaagttcg agggcgacac cctcgtcaac cgcatcgagc
     4381 tcaagggcat cgacttcaag gaggacggca acatcctcgg ccacaagctc gagtacaact
     4441 acaactctca caacgtctac atcatggccg acaagcaaaa gaacggcatc aaggtcaact
     4501 tcaagatccg ccacaacatc gaggacggct ctgtccaact cgccgaccac taccaacaaa
     4561 acacccctat cggcgacggc cctgtcctcc tccctgacaa ccactacctc tctacccaat
     4621 ctgccctctc taaggaccct aacgagaagc gcgaccacat ggtcctcctc gagttcgtca
     4681 ccgccgccgg catcacccac ggcatggacg agctctacaa gtctgctggc tctgctggca
     4741 tgtctaaggg cgaggagctc ttcaccggcg tcgtccctat cctcgtcgag ctcgacggcg
     4801 acgtcaacgg ccacaagttc tctgtctctg gcgagggcga gggcgacgcc acctacggca
     4861 agctcaccct caagttcatc tgcaccaccg gcaagctccc tgtcccttgg cctaccctcg
     4921 tcaccacctt cggctacggc gtccaatgct tcgcccgcta ccctgaccac atgaagcaac
     4981 acgacttctt caagtctgcc atgcctgagg gctacgtcca agagcgcacc atcttcttca
     5041 aggacgacgg caactacaag acccgcgccg aggtcaagtt cgagggcgac accctcgtca
     5101 accgcatcga gctcaagggc atcgacttca aggaggacgg caacatcctc ggccacaagc
     5161 tcgagtacaa ctacaactct cacaacgtct acatcatggc cgacaagcaa aagaacggca
     5221 tcaaggtcaa cttcaagatc cgccacaaca tcgaggacgg ctctgtccaa ctcgccgacc
     5281 actaccaaca aaacacccct atcggcgacg gccctgtcct cctccctgac aaccactacc
     5341 tctctaccca atctgccctc tctaaggacc ctaacgagaa gcgcgaccac atggtcctcc
     5401 tcgagttcgt caccgccgcc ggcatcaccc acggcatgga cgagctctac aagggtggat
     5461 cctaggcgcc gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     5521 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
     5581 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat
     5641 tgaccacacc tctaccggca gatccgctag ggataacagg gtaatacata tcactgacat
     5701 ttgagaagct gggatattgt tcaggcagga cgaggatgtg tgcttttcta cggtcaggaa
     5761 ccgacaggaa acatcgaagc ctccgatacg gaggacagac tggaaactga attgaggatc
     5821 atatttggag gactactttt tcaaggcgca tgttggagtg aagatacaaa aaagtgtaga
     5881 gcagcttggg attcatccat catcaccatc atcttcataa tcactttacc tccgtctcaa
     5941 ctcatgagtt ggtccgattg accttgggag ctgactacca acaatggttc ggatcgcacg
     6001 gcacaagatt tgattgatca gcgagctggt ccctgatgtg tccggatgat ttgtgttgta
     6061 tacggagggc tccacaatga ttgagaaacg gcaagacttg ctgcaagatt cggcactcac
     6121 gatcgaccat gcgagaagag cttgtaggcc gctgaacgaa ttgggtgata tcgcctaccc
     6181 atttaagaac agtgacgaag acatcacaag caaaccatat tcgaagatcc ggtttgagtg
     6241 aatgaatcaa aaaagatgaa agggacgtag gcgtgaacat gcgttaagcg ggctttgaaa
     6301 gtgcgtctcc ctctctttct cttttatgcc tcgatagctg ttgaacactc tctcgcctgc
     6361 aatcacctac ttcattctgc tcttctacaa gaagccggtc caaacaagtg gaagcccata
     6421 aggatatatg gacgtgaccc gtatgataga acaatgttat gctcggtttc gattctgtca
     6481 aactatggat atgtggttga gctatgtcta ggctgcccaa agcctctcgt tagccatgaa
     6541 caggtctctg gtatcaagat gtttgaacaa atgactttga gcttgtcgat tcttgtagtg
     6601 ttggtgcatg ctagctccag cagaatcttt cttccatacc aacggtttct tgacctttga
     6661 ccaagcatag tcatgtctgc actcctgcca gcaccaccaa catacgccga cttcacctag
     6721 agcaagaacc aagagatcta atagaggcag gatgcgtgtg cgaatgccac agtgatgatg
     6781 gtttttgtct acctgatact cgggagcttg tttgaagctg tcgacaagat ctaggacgtc
     6841 gccatcgtag ggttcaggcg gatctgaggt tgcaaagatg gtgctttgca aggaaagagt
     6901 gttgatacgt ttgaagaatt tgatggtctg acccaattgg aacaaatcgc attcgggcga
     6961 ggagtcgtag cccagcttac actgtcgctc gcgagacgtg tataggagct ccagcttttg
     7021 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7081 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7141 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7201 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7381 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7441 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7501 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7561 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7621 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7681 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7741 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7801 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7861 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7921 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7981 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8101 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8161 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8221 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8281 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8341 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8401 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8461 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8521 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8581 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8641 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8701 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8761 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8821 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8881 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8941 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9001 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9061 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9181 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     9241 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9301 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9361 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9421 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9481 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9541 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9601 ttggttaaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9661 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9721 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9781 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     9841 atacgactca ctatagggcg aattgggtac cgggccctga gtgttggtgt acgcgcatac
     9901 acaatgatcc gcggaaggat gttccccctg cggtggcaca gaacaggcaa tctaacaagc
     9961 tcgcccttgc tgcaaggcac agaggagctg aagccggcgc atgattgttg aagatctcca
    10021 ggtcttcgac agcaagcata aaggcaaaac gaaacgggcg cagagtcgca aaccgagagc
    10081 tgcggaggga caagttgagc gacaagcggg ggcaggaatt gacgagcttc ctccatgcac
    10141 gtctcgcgga ccggaagctg ctgcgaccca cacgggtgtc gttgggactg gcctagaaag
    10201 gatttacatg gcttggcttg ggctggcaag gaagactccg aagagtggag tgttctcagg
    10261 cagttcagag tcaagatcag gaaggtgcag acagtgaaga ggagtgttga tggatgcagg
    10321 gatcgctagc agggctgcga tagacgggga agagcgaatc cgagcagagg tggtctctga
    10381 ggtcgcagtg cactacctgt caatggccct agaccctgca ttggcgtgcg actgcggtca
    10441 agcagagtct tgtgttttgg tggacacttg cggcctcgaa tatgcagcag cggcaggttt
    10501 caagcagcat ccgaagccct cgggtcccac cccacctctg gaggcttttg agcagcaatg
    10561 gcaggtggca ggagtcggaa gagcgaaact ggctcccgca cgctgcttgt cagaaggaag
    10621 cataaagatg gacatgtaga cagcagcctc gctctttgct gctttccttt cccaactgcc
    10681 agagcctccc ccagcctgac cgtccttgag ttgtggcttg ttgacttgtt gacttgttga
    10741 cttgttgact tgtgattgtg tactgcacat ctccggccgt gtcctctcgc tttgacttgc
    10801 tcggcgcaaa atctcgactg ctctcagtgg tctgggctgg tgctcctcgg tcgtcattta
    10861 acattttgct tgcaacttcc catctggtgc ttcttgcgta caagcatcct tccctctcaa
    10921 ttgccttgat ccatagctat tcgacagcta cccagtcgcc cagcctcccg catctcttga
    10981 ccggcttcgt ccacgcacct cccacactcc cacaaagtct cgctcgacgt cgacgcccgc
    11041 aatgtcccgt cacgcaacaa tcacccagtc ataccaggag cgagcctcgc tgcccaagac
    11101 atcaccggtc gcctcgtacc tcctacgatt gatcgcagcg aagcagacaa atctctgtgt
    11161 ctcggcagat gtgtcgacaa ccagagagct gctgcaactc gccgaagagg tcggcgactc
    11221 aatatgcatg ctcaagacac acgccgacat cattaatgac tttggccctc gcaccatcga
    11281 gggcctgaaa gagattgctg ccaagaagca cttcctcgtc ttcgaggacc gcaagtttgg
    11341 cgacattggc agtaggtgcc tgccccaccc gtgtcttcca atcaccgacc actgacacag
    11401 cttcttgcag gtaccgtgca aaagcagttc actgccggcc ctctgcagat cgttcgctgg
    11461 gccaatatca tcaatgccca catcttccct ggccccgcca tcatcactgc tctctcgcaa
    11521 gccgcccacg atgccgtcag ttctctcaac actgctgtca ccacttccat ctcggcctcg
    11581 cccgttccct catacatgga cgacagcgac gaagtagact cgcctgcact gtctcaccac
    11641 gaagactcag acgatctcga cagactctcg agcgacgatg actccaacca attgtctaca
    11701 tacagtgacc ccactggtcg caagccaagc gtcgtttccg tatctaccac tatcagcacc
    11761 aagacagaaa gcatctcacc ccagcctacg ccaaaccacc acggtggccc caacgattct
    11821 atttcgtcca tcagcgccgg ctcggctggt caagaatcgt ctgctttggc ccgcctcgga
    11881 gagccccctc tcctccgaag cttgctgatc ctcgcagaga tgagcagtgc aggcaacttg
    11941 atgactggct catacactga acaatgtgtt gtggaagcac gcaagaaccc ggagttcgtc
    12001 atgggcttca tcgcacaacg cactctcaat gagcagcctg gtgacaattt catcaccatg
    12061 actcctggcg tccagattgg cgccactgga gatggtctcg gccaacagta caacactccg
    12121 gaaaaggtca tcggcgaagc tggcaccgac atcatcattg tcggccgcgg tgtctacggt
    12181 gctcaagata agagggccaa ggccgaggaa tacagagtca gagcatggaa ggcatacgag
    12241 gggagaattg gcgctgctac tgcaacaaaa ggtagcaggt gagaattcac tgacaataaa
    12301 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
    12361 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
    12421 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
    12481 atactgcccg ggatgttaca tgcgtacacg cgtttgtaca g
//
