LOCUS       sequence-468488-       12538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for integration at the native URA3 locus with 3xmCherry 
            under control of the ScACT1 promoter and 3xGFP under control of the 
            ApTUB1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Colarusso AV, Williams AM, Gladfelter AS, Wirshing ACE, Lew DJ
  TITLE     Optimized vectors for genetic engineering of Aureobasidium 
            pullulans.
  JOURNAL   Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi: 10.1091/mbc.E25-02-0059. 
            Epub 2025 Apr 9.
  PUBMED    40202829
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2025-06-1"; volume: "36(6):mr5. doi"; pages: " 
            10.1091/mbc.E25-02-0059. Epub 2025 Apr 9"
COMMENT     Sequence Label: pAP-U2-4
FEATURES             Location/Qualifiers
     source          1..12538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(2396..2583)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2504..2528
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7921..7939)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(8106..8303)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(10734..10753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10735..10753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10763..10780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10763..10779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10772..10794)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10920..11376)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11058..11079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11270..11289
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11403..11507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11508..11576,11577..12368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11726..11745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcct atacacgtct cgcgagcgac agtgtaagct
     1081 gggctacgac tcctcgcccg aatgcgattt gttccaattg ggtcagacca tcaaattctt
     1141 caaacgtatc aacactcttt ccttgcaaag caccatcttt gcaacctcag atccgcctga
     1201 accctacgat ggcgacgtcc tagatcttgt cgacagcttc aaacaagctc ccgagtatca
     1261 ggtagacaaa aaccatcatc actgtggcat tcgcacacgc atcctgcctc tattagatct
     1321 cttggttctt gctctaggtg aagtcggcgt atgttggtgg tgctggcagg agtgcagaca
     1381 tgactatgct tggtcaaagg tcaagaaacc gttggtatgg aagaaagatt ctgctggagc
     1441 tagcatgcac caacactaca agaatcgaca agctcaaagt catttgttca aacatcttga
     1501 taccagagac ctgttcatgg ctaacgagag gctttgggca gcctagacat agctcaacca
     1561 catatccata gtttgacaga atcgaaaccg agcataacat tgttctatca tacgggtcac
     1621 gtccatatat ccttatgggc ttccacttgt ttggaccggc ttcttgtaga agagcagaat
     1681 gaagtaggtg attgcaggcg agagagtgtt caacagctat cgaggcataa aagagaaaga
     1741 gagggagacg cactttcaaa gcccgcttaa cgcatgttca cgcctacgtc cctttcatct
     1801 tttttgattc attcactcaa accggatctt cgaatatggt ttgcttgtga tgtcttcgtc
     1861 actgttctta aatgggtagg cgatatcacc caattcgttc agcggcctac aagctcttct
     1921 cgcatggtcg atcgtgagtg ccgaatcttg cagcaagtct tgccgtttct caatcattgt
     1981 ggagccctcc gtatacaaca caaatcatcc ggacacatca gggaccagct cgctgatcaa
     2041 tcaaatcttg tgccgtgcga tccgaaccat tgttggtagt cagctcccaa ggtcaatcgg
     2101 accaactcat gagttgagac ggaggtaaag tgattatgaa gatgatggtg atgatggatg
     2161 aatcccaagc tgctctacac ttttttgtat cttcactcca acatgcgcct tgaaaaagta
     2221 gtcctccaaa tatgatcctc aattcagttt ccagtctgtc ctccgtatcg gaggcttcga
     2281 tgtttcctgt cggttcctga ccgtagaaaa gcacacatcc tcgtcctgcc tgaacaatat
     2341 cccagcttct caaatgtcag tgatatgtat taccctgtta tccctagcgg atctgccggt
     2401 agaggtgtgg tcaataagag cgacctcata ctatacctga gaaagcaacc tgacctacag
     2461 gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt
     2521 gtatacactt atttttttta taacttattt aataataaaa atcataaatc ataagaaatt
     2581 cgcggcgcct aggatccacc cttgtagagc tcgtccatgc cgtgggtgat gccggcggcg
     2641 gtgacgaact cgaggaggac catgtggtcg cgcttctcgt tagggtcctt agagagggca
     2701 gattgggtag agaggtagtg gttgtcaggg aggaggacag ggccgtcgcc gataggggtg
     2761 ttttgttggt agtggtcggc gagttggaca gagccgtcct cgatgttgtg gcggatcttg
     2821 aagttgacct tgatgccgtt cttttgcttg tcggccatga tgtagacgtt gtgagagttg
     2881 tagttgtact cgagcttgtg gccgaggatg ttgccgtcct ccttgaagtc gatgcccttg
     2941 agctcgatgc ggttgacgag ggtgtcgccc tcgaacttga cctcggcgcg ggtcttgtag
     3001 ttgccgtcgt ccttgaagaa gatggtgcgc tcttggacgt agccctcagg catggcagac
     3061 ttgaagaagt cgtgttgctt catgtggtca gggtagcggg cgaagcattg gacgccgtag
     3121 ccgaaggtgg tgacgagggt aggccaaggg acagggagct tgccggtggt gcagatgaac
     3181 ttgagggtga gcttgccgta ggtggcgtcg ccctcgccct cgccagagac agagaacttg
     3241 tggccgttga cgtcgccgtc gagctcgacg aggataggga cgacgccggt gaagagctcc
     3301 tcgcccttag acatgccagc agagccagca gacttgtaga gctcgtccat gccgtgggtg
     3361 atgccggcgg cggtgacgaa ctcgaggagg accatgtggt cgcgcttctc gttagggtcc
     3421 ttagagaggg cagattgggt agagaggtag tggttgtcag ggaggaggac agggccgtcg
     3481 ccgatagggg tgttttgttg gtagtggtcg gcgagttgga cagagccgtc ctcgatgttg
     3541 tggcggatct tgaagttgac cttgatgccg ttcttttgct tgtcggccat gatgtagacg
     3601 ttgtgagagt tgtagttgta ctcgagcttg tggccgagga tgttgccgtc ctccttgaag
     3661 tcgatgccct tgagctcgat gcggttgacg agggtgtcgc cctcgaactt gacctcggcg
     3721 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcttggac gtagccctca
     3781 ggcatggcag acttgaagaa gtcgtgttgc ttcatgtggt cagggtagcg ggcgaagcat
     3841 tggacgccgt agccgaaggt ggtgacgagg gtaggccaag ggacagggag cttgccggtg
     3901 gtgcagatga acttgagggt gagcttgccg taggtggcgt cgccctcgcc ctcgccagag
     3961 acagagaact tgtggccgtt gacgtcgccg tcgagctcga cgaggatagg gacgacgccg
     4021 gtgaagagct cctcgccctt agacatgcca gagccgccag agcccttgta gagctcgtcc
     4081 atgccgtggg tgatgccggc ggcggtgacg aactcgagga ggaccatgtg gtcgcgcttc
     4141 tcgttagggt ccttagagag ggcagattgg gtagagaggt agtggttgtc agggaggagg
     4201 acagggccgt cgccgatagg ggtgttttgt tggtagtggt cggcgagttg gacagagccg
     4261 tcctcgatgt tgtggcggat cttgaagttg accttgatgc cgttcttttg cttgtcggcc
     4321 atgatgtaga cgttgtgaga gttgtagttg tactcgagct tgtggccgag gatgttgccg
     4381 tcctccttga agtcgatgcc cttgagctcg atgcggttga cgagggtgtc gccctcgaac
     4441 ttgacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcttgg
     4501 acgtagccct caggcatggc agacttgaag aagtcgtgtt gcttcatgtg gtcagggtag
     4561 cgggcgaagc attggacgcc gtagccgaag gtggtgacga gggtaggcca agggacaggg
     4621 agcttgccgg tggtgcagat gaacttgagg gtgagcttgc cgtaggtggc gtcgccctcg
     4681 ccctcgccag agacagagaa cttgtggccg ttgacgtcgc cgtcgagctc gacgaggata
     4741 gggacgacgc cggtgaagag ctcctcgccc ttagacatct taagtttgtc aagagaaggg
     4801 gaagtgagga aagcacgacc cttcttgttg ttgtttacag ctagggtctt ggcgcacctt
     4861 ccctcttcaa cccgcttctt gcaccccagc ctccagccaa tgacccgtcc aagaagctat
     4921 atcactgctg agtctgccta actgattctt gcagactgat taatcactca gatcgatgct
     4981 tcgaccggga catttaatca aatctgttct tctgctgcct ctgacccatc gatgatgctc
     5041 atcctgtcta tcttgctcta cacatgcttc ctggccgctc ctgccatacc cagcgctggg
     5101 aacatatata cacaattaca gtaacaataa caagaggaca gatactacca aaatgtgtgg
     5161 ggaagcgggt aagctgccac agcaattaat gcacaacatt taacctacat tcttccttat
     5221 cggatcttca aaacccttaa aaacatatgc ctcaccctaa catattttcc aattaaccct
     5281 caatatttct ctgtcacccg gcctctattt tccattttct tctttacccg ccacgcgttt
     5341 ttttctttca aatttttttc ttccttcttc tttttcttcc acgtcctctt gcataaataa
     5401 ataaaccgtt ttgaaaccaa actcgcctct ctctctcctt tttgaaatat ttttgggttt
     5461 gtttgatcct ttccttccca atctctcttg tttaatatat attcatttat atcacgctct
     5521 ctttttatct tccttttttt cctctctctt gtattcttcc ttcccctttc tactcaaacc
     5581 aagaagaaaa agaaaaggtc aatctttgtt aaagaatagg atcttctact acatcagctt
     5641 ttagattttt cacgcttact gcttttttct tcccaagatc gaaaatttac tgaattaaca
     5701 ttaattaaac atggtctcta agggcgagga ggacaacatg gccatcatca aggagttcat
     5761 gcgcttcaag gtccacatgg agggctctgt caacggccac gagttcgaga tcgagggcga
     5821 gggcgagggc cgcccttacg agggcaccca aaccgccaag ctcaaggtca ccaagggcgg
     5881 ccctctccct ttcgcctggg acatcctctc tcctcaattc atgtacggct ctaaggccta
     5941 cgtcaagcac cctgccgaca tccctgacta cctcaagctc tctttccctg agggcttcaa
     6001 gtgggagcgc gtcatgaact tcgaggacgg cggcgtcgtc accgtcaccc aagactcttc
     6061 tctccaagac ggcgagttca tctacaaggt caagctccgc ggcaccaact tcccttctga
     6121 cggccctgtc atgcaaaaga agaccatggg ctgggaggcc tcttctgagc gcatgtaccc
     6181 tgaggacggc gccctcaagg gcgagatcaa gcaacgcctc aagctcaagg acggcggcca
     6241 ctacgacgcc gaggtcaaga ccacctacaa ggccaagaag cctgtccaac tccctggcgc
     6301 ctacaacgtc aacatcaagc tcgacatcac ctctcacaac gaggactaca ccatcgtcga
     6361 gcaatacgag cgcgccgagg gccgccactc taccggcggc atggacgagc tctacaaggg
     6421 ctctggcggc tctggcatgg tctctaaggg cgaggaggac aacatggcca tcatcaagga
     6481 gttcatgcgc ttcaaggtcc acatggaggg ctctgtcaac ggccacgagt tcgagatcga
     6541 gggcgagggc gagggccgcc cttacgaggg cacccaaacc gccaagctca aggtcaccaa
     6601 gggcggccct ctccctttcg cctgggacat cctctctcct caattcatgt acggctctaa
     6661 ggcctacgtc aagcaccctg ccgacatccc tgactacctc aagctctctt tccctgaggg
     6721 cttcaagtgg gagcgcgtca tgaacttcga ggacggcggc gtcgtcaccg tcacccaaga
     6781 ctcttctctc caagacggcg agttcatcta caaggtcaag ctccgcggca ccaacttccc
     6841 ttctgacggc cctgtcatgc aaaagaagac catgggctgg gaggcctctt ctgagcgcat
     6901 gtaccctgag gacggcgccc tcaagggcga gatcaagcaa cgcctcaagc tcaaggacgg
     6961 cggccactac gacgccgagg tcaagaccac ctacaaggcc aagaagcctg tccaactccc
     7021 tggcgcctac aacgtcaaca tcaagctcga catcacctct cacaacgagg actacaccat
     7081 cgtcgagcaa tacgagcgcg ccgagggccg ccactctacc ggcggcatgg acgagctcta
     7141 caagtctgct ggctctgctg gcatggtctc taagggcgag gaggacaaca tggccatcat
     7201 caaggagttc atgcgcttca aggtccacat ggagggctct gtcaacggcc acgagttcga
     7261 gatcgagggc gagggcgagg gccgccctta cgagggcacc caaaccgcca agctcaaggt
     7321 caccaagggc ggccctctcc ctttcgcctg ggacatcctc tctcctcaat tcatgtacgg
     7381 ctctaaggcc tacgtcaagc accctgccga catccctgac tacctcaagc tctctttccc
     7441 tgagggcttc aagtgggagc gcgtcatgaa cttcgaggac ggcggcgtcg tcaccgtcac
     7501 ccaagactct tctctccaag acggcgagtt catctacaag gtcaagctcc gcggcaccaa
     7561 cttcccttct gacggccctg tcatgcaaaa gaagaccatg ggctgggagg cctcttctga
     7621 gcgcatgtac cctgaggacg gcgccctcaa gggcgagatc aagcaacgcc tcaagctcaa
     7681 ggacggcggc cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcctgtcca
     7741 actccctggc gcctacaacg tcaacatcaa gctcgacatc acctctcaca acgaggacta
     7801 caccatcgtc gagcaatacg agcgcgccga gggccgccac tctaccggcg gcatggacga
     7861 gctctacaag agttaacaat caggcccctt ttcctttgtc gatatcatgt aattagttat
     7921 gtcacgctta cattcacgcc ctcctcccac atccgctcta accgaaaagg aaggagttag
     7981 acaacctgaa gtctaggtcc ctatttattt tttttaatag ttatgttagt attaagaacg
     8041 ttatttatat ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatc
     8101 ccgggcagta tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca
     8161 cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca
     8221 tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa
     8281 acaagaatct ttttattgtc agtgaattct cacctgctac cttttgttgc agtagcagcg
     8341 ccaattctcc cctcgtatgc cttccatgct ctgactctgt attcctcggc cttggccctc
     8401 ttatcttgag caccgtagac accgcggccg acaatgatga tgtcggtgcc agcttcgccg
     8461 atgacctttt ccggagtgtt gtactgttgg ccgagaccat ctccagtggc gccaatctgg
     8521 acgccaggag tcatggtgat gaaattgtca ccaggctgct cattgagagt gcgttgtgcg
     8581 atgaagccca tgacgaactc cgggttcttg cgtgcttcca caacacattg ttcagtgtat
     8641 gagccagtca tcaagttgcc tgcactgctc atctctgcga ggatcagcaa gcttcggagg
     8701 agagggggct ctccgaggcg ggccaaagca gacgattctt gaccagccga gccggcgctg
     8761 atggacgaaa tagaatcgtt ggggccaccg tggtggtttg gcgtaggctg gggtgagatg
     8821 ctttctgtct tggtgctgat agtggtagat acggaaacga cgcttggctt gcgaccagtg
     8881 gggtcactgt atgtagacaa ttggttggag tcatcgtcgc tcgagagtct gtcgagatcg
     8941 tctgagtctt cgtggtgaga cagtgcaggc gagtctactt cgtcgctgtc gtccatgtat
     9001 gagggaacgg gcgaggccga gatggaagtg gtgacagcag tgttgagaga actgacggca
     9061 tcgtgggcgg cttgcgagag agcagtgatg atggcggggc cagggaagat gtgggcattg
     9121 atgatattgg cccagcgaac gatctgcaga gggccggcag tgaactgctt ttgcacggta
     9181 cctgcaagaa gctgtgtcag tggtcggtga ttggaagaca cgggtggggc aggcacctac
     9241 tgccaatgtc gccaaacttg cggtcctcga agacgaggaa gtgcttcttg gcagcaatct
     9301 ctttcaggcc ctcgatggtg cgagggccaa agtcattaat gatgtcggcg tgtgtcttga
     9361 gcatgcatat tgagtcgccg acctcttcgg cgagttgcag cagctctctg gttgtcgaca
     9421 catctgccga gacacagaga tttgtctgct tcgctgcgat caatcgtagg aggtacgagg
     9481 cgaccggtga tgtcttgggc agcgaggctc gctcctggta tgactgggtg attgttgcgt
     9541 gacgggacat tgcgggcgtc gacgtcgagc gagactttgt gggagtgtgg gaggtgcgtg
     9601 gacgaagccg gtcaagagat gcgggaggct gggcgactgg gtagctgtcg aatagctatg
     9661 gatcaaggca attgagaggg aaggatgctt gtacgcaaga agcaccagat gggaagttgc
     9721 aagcaaaatg ttaaatgacg accgaggagc accagcccag accactgaga gcagtcgaga
     9781 ttttgcgccg agcaagtcaa agcgagagga cacggccgga gatgtgcagt acacaatcac
     9841 aagtcaacaa gtcaacaagt caacaagtca acaagccaca actcaaggac ggtcaggctg
     9901 ggggaggctc tggcagttgg gaaaggaaag cagcaaagag cgaggctgct gtctacatgt
     9961 ccatctttat gcttccttct gacaagcagc gtgcgggagc cagtttcgct cttccgactc
    10021 ctgccacctg ccattgctgc tcaaaagcct ccagaggtgg ggtgggaccc gagggcttcg
    10081 gatgctgctt gaaacctgcc gctgctgcat attcgaggcc gcaagtgtcc accaaaacac
    10141 aagactctgc ttgaccgcag tcgcacgcca atgcagggtc tagggccatt gacaggtagt
    10201 gcactgcgac ctcagagacc acctctgctc ggattcgctc ttccccgtct atcgcagccc
    10261 tgctagcgat ccctgcatcc atcaacactc ctcttcactg tctgcacctt cctgatcttg
    10321 actctgaact gcctgagaac actccactct tcggagtctt ccttgccagc ccaagccaag
    10381 ccatgtaaat cctttctagg ccagtcccaa cgacacccgt gtgggtcgca gcagcttccg
    10441 gtccgcgaga cgtgcatgga ggaagctcgt caattcctgc ccccgcttgt cgctcaactt
    10501 gtccctccgc agctctcggt ttgcgactct gcgcccgttt cgttttgcct ttatgcttgc
    10561 tgtcgaagac ctggagatct tcaacaatca tgcgccggct tcagctcctc tgtgccttgc
    10621 agcaagggcg agcttgttag attgcctgtt ctgtgccacc gcagggggaa catccttccg
    10681 cggatcattg tgtatgcgcg tacaccaaca ctcagggccc ggtacccaat tcgccctata
    10741 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    10801 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    10861 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
    10921 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
    10981 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
    11041 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
    11101 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
    11161 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
    11221 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
    11281 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
    11341 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
    11401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    11461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    11521 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    11581 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    11641 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    11701 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11761 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11821 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11881 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11941 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    12001 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    12061 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    12121 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    12181 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    12241 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    12301 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    12361 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    12421 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    12481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
