LOCUS       sequence-468500-       19562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in wheat plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19562)
  AUTHORS   Ni P, Zhao Y, Zhou X, Liu Z, Huang Z, Ni Z, Sun Q, Zong Y
  TITLE     Efficient and versatile multiplex prime editing in hexaploid wheat.
  JOURNAL   Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1.
  PUBMED    37386475
REFERENCE   2  (bases 1 to 19562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2023-06-29"; volume: "24(1):156. doi"; pages: " 
            10.1186/s13059-023-02990-1"
COMMENT     Sequence Label: pB-CMPE-ePPEplus
FEATURES             Location/Qualifiers
     source          1..19562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    733..752
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    908..927
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    934..1108
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        1115..3107
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3693..3749
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3753..3779
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3819..3839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             7853..7861
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8163..8183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9909..9929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10593..10609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10593..10609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10606..10628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10617..10633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10641..10647,10648..10665,10666..10671))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10686..10707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10898..11243
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11186..11206
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11294..11845
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11852..12026
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12104..12128
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13752..14546
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14633..15221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15122..15141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15375..15392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15407..15547
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15473..15492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15633..15655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15891..16085
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16151..17224)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17653..18282)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgcctgg cagacatact gtcccacaaa tgaagatgga
      301 atctgtaaaa gaaaacgcgt gaaataatgc gtctgacaaa ggttaggtcg gctgccttta
      361 atcaatacca aagtggtccc taccacgatg gaaaaactgt gcagtcggtt tggctttttc
      421 tgacgaacaa ataagattcg tggccgacag gtgggggtcc accatgtgaa ggcatcttca
      481 gactccaata atggagcaat gacgtaaggg cttacgaaat aagtaagggt agtttgggaa
      541 atgtccactc acccgtcagt ctataaatac ttagcccctc cctcattgtt aagggagcaa
      601 aatctcagag agatagtcct agagagagaa agagagcaag tagcctagaa gtagtcaagg
      661 cggcgaagta ttcaggcacg tggccaggaa gaagaaaagc caagacgacg aaaacaggta
      721 agagctaagc ttgttcactg ccgtataggc agcgagacca aggtctcagt ttaagagcta
      781 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
      841 caccgagtcg gtgcggagac caaggtctcc cgcggttcta tctagttacg cgttaaacca
      901 actagaagtt cactgccgta taggcaggga tcctttctcc ataataatgt gtgagtagtt
      961 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa
     1021 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     1081 accaaaatcc agtactaaaa tccagatcaa gcttctgcag tgcagcgtga cccggtcgtg
     1141 cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt
     1201 ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt taaactttac
     1261 tctacgaata atataatcta tagtactaca ataatatcag tgttttagag aatcatataa
     1321 atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg actctacagt
     1381 tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc acctatataa
     1441 tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt ttttatagac
     1501 taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga aaactaaaac
     1561 tctattttag tttttttatt taataattta gatataaaat agaataaaat aaagtgacta
     1621 aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt ttcttgtttc
     1681 gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac caaccagcga
     1741 accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg tcgctgcctc
     1801 tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg gcatccagaa
     1861 attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc ctcctctcac
     1921 ggcaccggca gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc
     1981 gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc
     2041 gcacacacac acaaccagat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta
     2101 cgccgctcgt cctccccccc cccccctctc taccttctct agatcggcgt tccggtccat
     2161 ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag
     2221 atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc
     2281 taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg
     2341 gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta
     2401 tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg
     2461 gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa
     2521 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     2581 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     2641 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     2701 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     2761 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     2821 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     2881 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     2941 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     3001 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     3061 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcagccc taggcttaag
     3121 tcatggacca ctacctcgac atcaggctca ggccagaccc agagttccca ccagcccagc
     3181 tcatgtccgt cctcttcggc aagctccacc aggccctcgt ggcccagggc ggcgacagga
     3241 tcggcgtgtc cttcccagac ctcgacgagt ccaggtccag gctcggcgag aggctccgca
     3301 tccacgcctc cgccgacgac ctcagggccc tcctcgccag gccgtggctg gagggcctca
     3361 gggaccacct ccagttcggc gagccagccg tggtgccaca cccaacccca tacaggcaag
     3421 tgtccagggt gcaagccaag tccaacccag agaggctcag gaggaggctc atgaggaggc
     3481 acgacctctc cgaggaagag gccaggaagc gcatcccaga caccgtggcc agggccctcg
     3541 acctcccatt cgtgaccctc aggtcccagt ccaccggcca gcacttccgc ctcttcatca
     3601 ggcacggccc actccaggtg accgccgagg agggcggctt tacctgctac ggcctctcca
     3661 agggcggctt cgtgccgtgg ttcggctccg gcgccaccaa cttctccctc ctcaagcaag
     3721 ccggcgacgt ggaggagaac ccaggcccaa tgccagccgc caagcgcgtc aagctggacg
     3781 gcggaaagcg caccgccgac ggatccgagt tcgagagccc taagaaaaag agaaaagtgg
     3841 acaagaagta ctcgatcggc ctcgatattg ggactaactc tgttggctgg gccgtgatca
     3901 ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt
     3961 ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgaggcta
     4021 cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc
     4081 tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg
     4141 aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca
     4201 ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc
     4261 tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga
     4321 ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg
     4381 acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta
     4441 atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggaagc
     4501 tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca
     4561 ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg
     4621 ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga
     4681 tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc
     4741 tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga
     4801 tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc
     4861 agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt
     4921 acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga
     4981 agatggacgg cacggaggag ctgctggtga agctcaagcg cgaggacctc ctgaggaagc
     5041 agcggacatt cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta
     5101 tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga
     5161 agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct
     5221 tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg
     5281 tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc
     5341 tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca
     5401 acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg
     5461 gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta
     5521 agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg
     5581 gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta
     5641 aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga
     5701 cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc
     5761 tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc
     5821 tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact
     5881 tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc
     5941 tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg
     6001 agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga
     6061 aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg
     6121 agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga
     6181 agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg
     6241 agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata
     6301 tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatgccatcg
     6361 ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca
     6421 agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact
     6481 actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa
     6541 aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg
     6601 tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta
     6661 agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc
     6721 tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc
     6781 accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc
     6841 caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga
     6901 tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca
     6961 ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc
     7021 tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga
     7081 ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga
     7141 ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc
     7201 gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact gtggcctact
     7261 cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg
     7321 agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc
     7381 tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact
     7441 cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc gagctgcaga
     7501 aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact
     7561 acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc
     7621 acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc
     7681 tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa
     7741 tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc ggcgcccctg
     7801 ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc actaaggagg
     7861 tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc
     7921 tgtctcagct cgggggcgac gaattctccg ggagcgagac gccaggcacc tccgagtcgg
     7981 ccaccccaga atctgccaca gtggtgtccg gccaaaagca ggaccgccag ggcggagaac
     8041 gcagaaggtc ccagctcgat agggatcagt gtgcctactg caaggagaag ggccactggg
     8101 ccaaagactg cccgaaaaag ccgcgcggcc cacgcggccc aaggccacaa acatccctcc
     8161 ttccaaagaa gaagcggaag gtggagctca gcggaggatc ttccggagga tctagcggct
     8221 ccgagacacc aggaacatcc gaaagcgcta caccagaatc tagcggaggc tcttccggag
     8281 gatctaggcc taccctcaac atcgaggatg agtatcgcct ccacgaaacc tccaaagaac
     8341 cggacgtgtc cctcggcagc acatggctca gcgacttccc acaagcgtgg gccgaaaccg
     8401 gcggcatggg cctcgccgtc cgccaagccc cactcattat cccgctgaag gcgacctcca
     8461 caccggtgtc catcaagcag tacccgatga gccaagaggc gaggctcggg attaagccgc
     8521 acattcagcg cctcctcgat caaggcattc tcgtgccgtg ccaatccccg tggaatacac
     8581 cactcctccc ggtcaaaaag ccgggcacca acgactatcg cccggtccaa gatctccgcg
     8641 aggtcaacaa gcgcgtggaa gacatccacc cgaccgtccc gaacccgtat aatctgctct
     8701 ccgggctccc accatcccac cagtggtata cagtgctgga cctcaaagac gccttcttct
     8761 gtctccgcct ccacccaaca agccagccgc tcttcgcctt cgagtggcgc gacccggaga
     8821 tgggcatctc cggccaactg acatggacac gcctcccgca aggcttcaag aacagcccga
     8881 cactcttcaa cgaggcgctc catagggacc tcgcggattt tcgcatccag catccggacc
     8941 tcatcctcct ccagtatgcc gatgatctcc tcctcgccgc gacctccgag ctggattgtc
     9001 aacaaggcac acgcgcgctc ctccaaacac tcgggaacct cggctatcgc gcgtccgcga
     9061 aaaaggccca aatctgccag aagcaagtga agtacctcgg gtatctgctc aaggaaggcc
     9121 aacgctggct caccgaagcg cgcaaagaaa cagtgatggg gcaaccgaca ccgaaaacac
     9181 cacgccagct gcgcgagttt ctcggcaaag ccggcttctg tcgcctcttc atcccgggct
     9241 ttgccgagat ggccgcgcca ctctacccac tcaccaagcc gggcacactg tttaactggg
     9301 ggccggatca gcagaaagcc taccaagaga tcaaacaagc gctcctcacc gccccagcgc
     9361 tcgggctccc agatctcaca aagccgttcg agctgttcgt cgatgagaag caaggctacg
     9421 cgaagggcgt gctcacacag aagctcggcc cgtggaggag gccagtggcc tatctctcca
     9481 aaaaactcga tccagtggcc gccggctggc caccgtgtct gcgcatggtc gccgcgattg
     9541 ccgtgctcac aaaggatgcc ggcaaactca caatgggcca gccgctggtg atcctcgcgc
     9601 cacatgccgt ggaagccctc gtcaaacagc cgccggatag gtggctctcc aatgcgcgca
     9661 tgacccatta ccaagcgctc ctcctcgaca ccgatcgcgt ccagttcggc ccagtggtcg
     9721 ccctcaatcc ggcgacactg ctgccactcc cagaggaggg cctccaacac aactgtctgg
     9781 atattctcgc ggaagcgcat ggcacaaggc cagacctcac agatcaaccg ctcagcggcg
     9841 gcagcaaaag gaccgccgat tcccaacatt ccaccccacc aaaaaccaag aggaaggtgg
     9901 gctctggccc aaagaagaag aggaaggtct gaactagtag ctcagagctt tcgttcgtat
     9961 catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga
    10021 atcctactga gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg
    10081 tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg
    10141 gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt
    10201 gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt
    10261 gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta
    10321 aaacacttgt agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct
    10381 agaaaagctg caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga
    10441 actttccttt atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta
    10501 tagttatact catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact
    10561 tgccaattga ttgacaacga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
    10621 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    10681 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    10741 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10801 cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga
    10861 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
    10921 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
    10981 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
    11041 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
    11101 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
    11161 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    11221 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc
    11281 tctcgagtct accatgagcc cagaacgacg cccggccgac atccgccgtg ccaccgaggc
    11341 ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca cggtcaactt
    11401 ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg
    11461 ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc
    11521 ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg tctccccccg
    11581 ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt ccctggaggc
    11641 acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat
    11701 gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct tcaagcacgg
    11761 gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac cgccccgtcc
    11821 ggtcctgccc gtcaccgaga tttgactcga gtttctccat aataatgtgt gagtagttcc
    11881 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
    11941 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    12001 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
    12061 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    12121 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
    12181 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
    12241 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
    12301 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
    12361 tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
    12421 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
    12481 ggagttcgtc ttgttataat tagggaaggt gcgaataagc ggggaaattc ttctcggctg
    12541 actcagtcat ttcatttctt catgtttgag ccgatttttt ctcccgtaaa tgccttgaat
    12601 cagcctattt agaccgtttc ttcgccattt aaggcgttat ccccagtttt tagtgagatc
    12661 tctcccactg acgtatcatt tggtccgccc gaaacaggtt ggccagcgtg aataacatcg
    12721 ccagttggtt atcgtttttc agcaacccct tgtatctggc tttcacgaag ccgaactgtc
    12781 gcttgatgat gcgaaatggg tgctccaccc tggcccggat gctggctttc atgtattcga
    12841 tgttgatggc cgttttgttc ttgcgtggat gctgtttcaa ggttcttacc ttgccggggc
    12901 gctcggcgat cagccagtcc acatccacct cggccagctc ctcgcgctgt ggcgcccctt
    12961 ggtagccggc atcggctgag acaaattgct cctctccatg cagcagatta cccagctgat
    13021 tgaggtcatg ctcgttggcc gcggtggtga ccaggctgtg ggtcaggcca ctcttggcat
    13081 cgacaccaat gtgggccttc atgccaaagt gccactgatt gcctttcttg gtctgatgca
    13141 tctccggatc gcgttgctgc tctttgttct tggtcgagct gggtgcctca atgatggtgg
    13201 catcgaccaa ggtgccttga gtcatcatga cgcctgcttc ggccagccag cgattgatgg
    13261 tcttgaacaa ttggcgggcc agttgatgct gctccagcag gtggcggaaa ttcatgatgg
    13321 tggtgcggtc cggcaaggcg ctatccaggg ataaccgggc aaacagacgc atggaggcga
    13381 tttcgtacag agcatcttcc atcgcgccat cgctcaggtt gtaccaatgc tgcatgcagt
    13441 gaatgcgtag catggtttcc agcggataag gtcgccggcc attaccagcc ttggggtaaa
    13501 acggctcgat gacttccacc atgttttgcc atggcagaat ctgctccatg cgggacaaga
    13561 aaatctcttt tctggtctga cggcgcttac tgctgaattc actgtcggcg aaggtaagtt
    13621 gatgactcat gatgaaccct gttctatggc tccagatgac aaacatgatc tcatatcagg
    13681 gacttgttcg caccttcctt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
    13741 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
    13801 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
    13861 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
    13921 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
    13981 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
    14041 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
    14101 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
    14161 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
    14221 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
    14281 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
    14341 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
    14401 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
    14461 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
    14521 atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg
    14581 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    14641 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    14701 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    14761 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    14821 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    14881 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    14941 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    15001 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    15061 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    15121 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    15181 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    15241 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    15301 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    15361 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
    15421 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
    15481 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
    15541 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    15601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    15661 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
    15721 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
    15781 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
    15841 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
    15901 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
    15961 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
    16021 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
    16081 ccacaggaaa cagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
    16141 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
    16201 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
    16261 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
    16321 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
    16381 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
    16441 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
    16501 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
    16561 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
    16621 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    16681 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    16741 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    16801 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    16861 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    16921 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    16981 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    17041 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    17101 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    17161 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    17221 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
    17281 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    17341 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    17401 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    17461 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    17521 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    17581 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    17641 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    17701 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    17761 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    17821 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    17881 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    17941 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    18001 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    18061 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    18121 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    18181 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    18241 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    18301 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    18361 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    18421 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    18481 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    18541 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    18601 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    18661 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    18721 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    18781 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    18841 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    18901 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    18961 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    19021 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    19081 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    19141 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    19201 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    19261 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    19321 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    19381 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    19441 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    19501 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    19561 aa
//
