LOCUS       sequence-468512-       10383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pEXPA1:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..63
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      151..861
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1156..1947
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2288..2876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2777..2796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3212..4360
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4506..4530
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5032..5377
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5320..5340
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5468..6262
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5522..5541)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6132..6151
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6275..6527
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      10104..10356
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
       61 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
      121 ttatccgttc gtccatttgt atgtgccagc cgcctttgcg acgctcaccg ggctggttgc
      181 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
      241 gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct
      301 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
      361 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
      421 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
      481 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
      541 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
      601 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
      661 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
      721 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
      781 cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg
      841 gcctcacccc aaaaatggca gcgctggcca attcccgagg cacgaaccca gtggacataa
      901 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
      961 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1021 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1081 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     1141 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     1201 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     1261 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
     1321 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     1381 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1441 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1501 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1561 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1621 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1681 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1741 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1801 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1861 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtgaaaggc
     1921 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     1981 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     2041 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     2101 ttgggagata tatcatgctg tcagaccaag tttactcata tatactttag attgatttaa
     2161 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2881 ccagcaacgc ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg
     2941 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     3001 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     3061 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     3121 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     3181 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     3241 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     3301 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     3361 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     3421 ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat
     3481 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     3541 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     3601 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     3661 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     3721 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     3781 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     3841 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     3901 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     3961 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     4021 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     4081 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     4141 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     4201 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     4261 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     4321 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     4381 ggctgggggt tcagcagccc ctgctcggat ctgttggacc ggacagtagt catggttgat
     4441 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     4501 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     4561 ttgcggacgt ttttaatgta ctggggttga acactctgtg ggtctcatgc cgaattcgga
     4621 tccggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     4681 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     4741 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     4801 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     4861 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
     4921 tccaaccacg tctacaaagc aagtggattg atgtgataac atggtggagc acgacactct
     4981 ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt
     5041 tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     5101 catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     5161 aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac
     5221 gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg
     5281 tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     5341 ctctatataa ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt
     5401 ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat
     5461 cgatacaatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5521 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5581 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5641 tgaactccaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5701 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5761 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5821 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5881 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5941 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat
     6001 gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt
     6061 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6121 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6181 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6241 ccttcttgac gagttcttct gagcttgtca agcagatcgt tcaaacattt ggcaataaag
     6301 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6361 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6421 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6481 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctgcaagaa
     6541 ttcaagcttg gagacactaa tcatttgtag atgaaaatca ttatcaaaca caattcaaag
     6601 aggacatatt cgtgtcaact ttcttccgct catctatcaa tacatgctta caacgttagc
     6661 tcgaaacggc gtctgtcatc cacatgcgga gctatctctg tgtttttcat ttatttatct
     6721 tctacttatt aatagatttt tgttttgttt ctctcagaaa atattactaa ttgttaaaag
     6781 atgattattt tgaagaacta gcaaacggac cgtccatgat attttgtgga ccatgttttg
     6841 agctgaaatt acggaaactt ggccgtcgat ttctgccgtt gaacttcttc accttattaa
     6901 atttaatgtg catttatttt ctcgatatgc aaactaaaaa aatgtgactc taaaaaaaat
     6961 ctatctaacc ctaataaata catttgcaga aacaccgtag aagctcctta aaagttagat
     7021 acttcatcac caaacacatg taaaaatttt attttgtcat taagtaattc aatatatgta
     7081 cttccagcga cattttttct cataaaccag acacgacttc ttcatcgatc gtacataggt
     7141 ttgatgattt tttaaaataa ataataagat ggttacgcat cgaaacatta gattgagata
     7201 aacaaacgac aaagctatac gtgtcgtggt acatcgatct caaagcagac cactatgacc
     7261 cacttggatt tcaacttgaa ttttctttgt ctttatgata ttttctttgc ttttcttttg
     7321 aatattcaat ttcacactca gtaataaaac tttctccagc ctccatattg accttaatcc
     7381 attacttcta catactatac caaattcaaa atttagaact acctattttg tagactaaaa
     7441 ctttctcaag cttaaattgt ttcttgatag actaaatata ttatcccaag gtaaagttgg
     7501 tgtttatgtg tgtttgaagg cgcctgaaaa aaatagaaac cacacgactc gtcttgtatg
     7561 tagctcacat gtgtctttgc ctctttgctt tttcatgcca ttaaatatta aatcttcagc
     7621 ggtttttggt ccccaacaat aaaaatatat tttcttacta tattctatag ttcattaata
     7681 ttacattgaa taaacctaat gttttttaat atgaaaaacc agaaaaataa aattgcattc
     7741 ggagttttgg tcttgttttg aaaagaaaag aaaaaagtgg aagctagttg cattaacatt
     7801 aaacagtggc aaaaacgtaa atcattcact cactaagttt ttctataaat tgaactactc
     7861 ccctcctctg ctttttccaa ttctaaacca aacaacagat tctcataatc atctcttctt
     7921 ttttcctctt tacgaaaaga agaaagatca aaccttccaa gtaatcattt tctttctctc
     7981 tctcacacac acacattcac tagttttagc ttcacaaaat gtgatctaac ttcatttacc
     8041 tatatgcagg tttacacaaa aagaaaaaag aacgaatggg tcagtccctt atgttacgtc
     8101 ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg
     8161 atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg
     8221 caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt cgtaattatg
     8281 cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggtaagtct tactctctct
     8341 tttttggtct gtatttttaa ttttttgaag tatactattt gtactgacgc taataatctt
     8401 ttttcaggtt gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc
     8461 aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa
     8521 gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac
     8581 aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag
     8641 aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg
     8701 ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa
     8761 gactgtaacc acgcgtctgt tgactggcag gtacttcatg cttcaacgtg taacttaaga
     8821 gatactgtgt gaaattttat atttccatac atttgcttga cctttgcttt ttgtcaattt
     8881 ttttcccctt acaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc
     8941 aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc
     9001 tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag
     9061 agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt
     9121 tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact
     9181 tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga
     9241 ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg
     9301 cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt
     9361 taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca
     9421 acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa
     9481 accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg
     9541 cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga
     9601 tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg
     9661 atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg
     9721 cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta
     9781 tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt
     9841 ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca
     9901 gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat
     9961 tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg
    10021 cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag
    10081 gcaaacaatg agcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat
    10141 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
    10201 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
    10261 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
    10321 aattatcgcg cgcggtgtca tctatgttac tagatcgacg ctactagagg atgcacatgt
    10381 gac
//
