LOCUS       sequence-468516-       11455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 11455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pGstA1+WIRa:Gus_introns
FEATURES             Location/Qualifiers
     source          1..11455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    38..62
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      150..860
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1155..1946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2287..2875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2776..2795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3211..4359
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4505..4529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5031..5376
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5319..5339
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5467..6261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5521..5540)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6131..6150
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6274..6526
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     6553..6569
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6553..6569
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      11175..11427
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
       61 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
      121 tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc
      181 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
      241 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      301 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
      361 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
      421 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
      481 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
      541 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
      601 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
      661 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
      721 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
      781 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
      841 cctcacccca aaaatggcag cgctggccaa ttcccgaggc acgaacccag tggacataag
      901 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      961 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1021 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1081 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1141 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1201 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1261 ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg
     1321 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1381 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1441 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1501 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1561 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1621 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1681 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1741 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1801 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1861 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtgaaaggcg
     1921 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     1981 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     2041 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     2101 tgggagatat atcatgctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc
     2941 gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc
     3001 cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc
     3061 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
     3121 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
     3181 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
     3241 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
     3301 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
     3361 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
     3421 gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg
     3481 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
     3541 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
     3601 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
     3661 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
     3721 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
     3781 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
     3841 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
     3901 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
     3961 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
     4021 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
     4081 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
     4141 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
     4201 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
     4261 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
     4321 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
     4381 gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc atggttgatg
     4441 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     4501 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     4561 tgcggacgtt tttaatgtac tggggttgaa cactctgtgg gtctcatgcc gaattcggat
     4621 ccggaggtca acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca
     4681 gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc
     4741 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt
     4801 ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc
     4861 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt
     4921 ccaaccacgt ctacaaagca agtggattga tgtgataaca tggtggagca cgacactctg
     4981 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     5041 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     5101 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     5161 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     5221 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     5281 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     5341 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
     5401 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     5461 gatacaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5641 gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg
     6001 cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa tatcatggtg
     6061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6241 cttcttgacg agttcttctg agcttgtcaa gcagatcgtt caaacatttg gcaataaagt
     6301 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     6361 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     6421 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     6481 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg ctgcaagaat
     6541 tcaagcttgg agtctagaac tagtggatcc ccgacgccga agtggagccg acagccccca
     6601 ggtcccaagc cctcggcaga ctagatcact agccctggat cggcgaggtg actggatgac
     6661 gagcagcacc tggtctggcg ggtgttgggc gagtagaacc aggggcgatg gcgacgcgct
     6721 gaccttctcc cctcaccggc gatctgctcc ttctgggtgg gggtcgccgg ctgacgttct
     6781 gttgcggggt gggggtcgcc ggctggcgtt ctgctgcggg gtgggagtcg ccgaccggcg
     6841 tgctgctgct aggacaatcg gtgaggccag ttaggtgcta gccgatcgat tggcgaagag
     6901 atccgagtcc tggggagatc agtgaggcca ggtgctattt ggcctatcaa ttggccaggt
     6961 tctgggaacg gggcgtggcg tgatcaacga ggtgctaggc tgctagctag ggaactggat
     7021 cctggaacgt ggaggaggca agtccggtat gctaagtact ttaactttcc ttcttcacat
     7081 ccacctgatt cagattattt tgatctaaat taacttgcaa aaaatatatg tgtgatatcc
     7141 atctactata attgcttaca atcaaaatta tatgtgattt tttttagttt agaagattta
     7201 tatgcacagt aaatctgaat gttcttcaca tgcatgattt agtttaactt taaagagtta
     7261 tactaactag tcttgataaa gagatctttt ggagcaacac caaacctcgt gaggtgtttt
     7321 gcctacggaa aggttgtgct atgtaatgat tattattagg atcaaagttg taggataaac
     7381 gtaaaacctt ctcgatgtat cttttataca acattgtagt ttagttatat atggagagag
     7441 tgatttaaca ctttgtgttt aagagtagaa taagttattc cacactctag ccaaacgaac
     7501 tatttggcaa atatctcgct agctggtgag agccagagcc gtggaaagtc tgtcttgcta
     7561 ttaaggcaca agcatcaaac aggaacattt agagccatgg aaaagtgatg tgtcgcctac
     7621 caatgggcca actgctagcg atgtaataat agcatccaag ttgatttttt atagaacatg
     7681 caaggcgttg gcaagtggga aaatgattga tcgctggcaa gcttaactct cggaacttat
     7741 agcattcaac tgaatcagaa caaagattaa aaaaaaatac atttccatcg atagtgaaaa
     7801 attattcaat tgagtgacaa cgaaaatcat attggaatgt acatttactt gttgatttta
     7861 aattagaggc atttttctac cttttttagt taataagata tgcatatacc cacccttagt
     7921 gttttcgaga caacgagagg gcacattgct tttggtgcta ccatctctct caagcctcaa
     7981 ataagttgtg cggacacgat tatcttcccg cgttggaata tcgtggcctg gtagagctag
     8041 cgaaaaatct tccatgttgg aatatgtcgg cagccggata gccgccatgc atgtaaagtc
     8101 tcttttacct ttacacttgc tcaagtgaca ctgtatgtcg cctaccactt gctaaatcaa
     8161 tgggccaact gctagcgacg taatagtagc aagttgattt acagtgtttt gctacagttc
     8221 tctgactttg tttcttcatt ttagactagc tgactactgt cgcttacctg ccttcccttc
     8281 tccacgttag aggatccagt tctgatattg acacctcgac gatgggagga agggcgcgat
     8341 cgatgtggag taatttgaat ttcaaatcta tctatctggg gtatattggt ccttcaccga
     8401 tgtttggggg gctgtcggaa attggttccg cgatctacaa aagtgaatgg agggagtagt
     8461 tgtttctcca atccgtacca acgcacgtgt ttctaactag tacttacttc cttcgcacca
     8521 caatatggaa tagagggagt atcgataaac taacaaagat gattacttac ccggtttaaa
     8581 tgattcaaga gctcatttaa tttggcactc atcatttcat atatcttttt tggtagaaat
     8641 gaaataaagc agatctagac actagctaaa aagtcgatgt agccttgtta tttccttggg
     8701 ccacgcgggc cgggtgtggt gctccctgct ctgtgtataa atggagatca acatccaagg
     8761 cctcctccca cacacacacg ctacagagca gagcagagtc ttgctccagt atctgccctc
     8821 tcctgcctgc ctgtagagca tccatcacgt gaagttcacg gacaaactac gtacacaggc
     8881 agctagctct cgaaacctcg ctcgaaacgc acctgcagat cgctctcttc gtcgtcgtcg
     8941 ccgcgatcat catcaacagc tccgtctgcc ttggagccac ggccgtccac gacgccgccg
     9001 cctcaggtca gtcgtcggac ggtgtccgtt catttcctcc ccatttttgt aattgattaa
     9061 cttgttatac atgctgacct cgacctgctg aataacgtcc gtccatggtt tcccgtccag
     9121 gcaccccggg ccactgtcca cacgaaatgg gtcagtccct tatgttacgt cctgtagaaa
     9181 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     9241 actgtggaat tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg
     9301 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg
     9361 tctggtatca gcgcgaagtc tttataccga aaggtaagtc ttactctctc ttttttggtc
     9421 tgtattttta attttttgaa gtatactatt tgtactgacg ctaataatct tttttcaggt
     9481 tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg
     9541 gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc
     9601 acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg
     9661 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag
     9721 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc
     9781 acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac
     9841 cacgcgtctg ttgactggca ggtacttcat gcttcaacgt gtaacttaag agatactgtg
     9901 tgaaatttta tatttccata catttgcttg acctttgctt tttgtcaatt tttttcccct
     9961 tacaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg
    10021 ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac ctctggcaac
    10081 cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca gagtgtgata
    10141 tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag ttcctgatta
    10201 accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac ttgcgtggca
    10261 aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg attggggcca
    10321 actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg gcagatgaac
    10381 atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct ttaggcattg
    10441 gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc aacggggaaa
    10501 ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa aaccacccaa
    10561 gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt gcacgggaat
    10621 atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg
    10681 tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt gatgtgctgt
    10741 gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg gcagagaagg
    10801 tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt atcatcaccg
    10861 aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg tggagtgaag
    10921 agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg
    10981 tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg
    11041 gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc
    11101 tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat
    11161 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    11221 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    11281 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    11341 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    11401 gcgcggtgtc atctatgtta ctagatcgac gctactagag gatgcacatg tgacc
//
