LOCUS       sequence-468517-       12305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Brassica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Bo_NPTII:pATML:Gus_introns
FEATURES             Location/Qualifiers
     source          1..12305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      129..839
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1134..1925
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2266..2854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2755..2774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3190..4338
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4484..4508
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5010..5355
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5298..5318
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5446..6240
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5500..5519)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6110..6129
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6253..6505
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      12004..12256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
       61 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
      121 gtgccagccg cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc
      181 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc
      241 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
      301 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
      361 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
      421 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
      481 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
      541 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
      601 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
      661 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
      721 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
      781 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
      841 gctggccaat tcccgaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
      901 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      961 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1021 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1081 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     1141 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1201 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     1261 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1321 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1381 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     1441 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1501 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1561 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1621 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1681 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1741 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1801 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     1861 cagatcagtt ggaagaattt gtccattacg tgaaaggcga gatcaccaag gtagtcggca
     1921 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     1981 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     2041 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgctgtc
     2101 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2881 gttcctggca gatcctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac
     2941 ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag
     3001 gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag
     3061 acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca
     3121 ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa
     3181 ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg
     3241 gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc
     3301 gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac
     3361 agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt
     3421 cgtcttgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact
     3481 atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc
     3541 aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg
     3601 ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct
     3661 gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt
     3721 ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat
     3781 gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg
     3841 atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat
     3901 tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc
     3961 gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt
     4021 ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct
     4081 ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga
     4141 cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc
     4201 cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa
     4261 gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg
     4321 gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagcccct
     4381 gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta tcgagtggtg
     4441 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
     4501 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
     4561 ggggttgaac actctgtggg tctcatgccg aattcggatc cggaggtcaa catggtggag
     4621 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     4681 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     4741 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     4801 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     4861 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     4921 gtggattgat gtgataacat ggtggagcac gacactctgg tctactccaa aaatgtcaaa
     4981 gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat aatttcggga
     5041 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
     5101 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatctc
     5161 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5221 gaggttccaa ccacgtctac aaagcaagtg gattgatgtg acatctccac tgacgtaagg
     5281 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     5341 catttggaga ggacacgctc gagtataaga gctcattttt acaacaatta ccaacaacaa
     5401 caaacaacaa acaacattac aattacattt acaattatcg atacaatgat tgaacaagat
     5461 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5521 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5581 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
     5641 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5701 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5761 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5821 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5881 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5941 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
     6001 gtgacccacg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6061 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6121 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6181 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6241 gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     6301 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     6361 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     6421 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     6481 gcggtgtcat ctatgttact agatcgacgc tgcaagaatt caagcttgga gttttacatt
     6541 gattctgaac tgtacccaat ttttttaaag cttatcaaag aaaaaacaag aacaaaacga
     6601 tgcatagttt ctaaaatgtg ctaaaattca gaaactgaaa catgattcat tgtctgaaac
     6661 tttgtttcaa attactgaaa ataatcattc actggaccaa aacaaataaa taaaataaaa
     6721 tcgaatttct gaatttggaa attggttttt ggtttttaat tttaaacaaa acaaaaacga
     6781 aatttgaagg caataaatga gttagttggt aggcagaagt cactcgttcc cactagctat
     6841 tattattaga agaaacgtcc ccacaactcc aaggcgtttc agttccttta atttactgaa
     6901 ttaccctcct catatctata aaaaatcacc tcttgtacca atgccccatt tacacatcct
     6961 gtcgtttatt tctagactaa gtggactaca tgtcggttat ttgattcgca ccatgcgtat
     7021 ttggattatc gctaacacac cccttcaaac aatacgctta actcgtatta caaaatttca
     7081 agtgatgaat tatctatgta taagatatag ataggaacaa ctaagcatcg agaaatttgt
     7141 atataaatca actagactta tatatatttc gatacagaat ttatacgcat tatatcaaat
     7201 taattagtaa ttgtttcctc tacgtgagtt taattaacaa tgataagcta cattgagtgt
     7261 atcagttcta aaactttata gtatgctaca atcaattttt ctaagtaaca acttcaagca
     7321 aggaatcaca cacacacagt ggtacataat aaacttgatt ttaatatcat atgatcagca
     7381 tcattaacgg aataagttaa gtaattcgtc atccatacta ctaagtcata ttaaaatcat
     7441 aatcaaactt aaaagccgat tagaaagaga gcaaatatat ctaaaaattc acgaggaata
     7501 cgacaaatgc aaggaaacac agctagtatt attaaactta atagatattg gatgaatgac
     7561 tgcataatat atatcacatt aaaagtggac ataaatttgc atatgtgtaa tgtacctctc
     7621 cacaattaat cgcggaccat ttattttact attacaagtc aagtaacttt atattgttga
     7681 tccataattc ttttcgaaca taaaatcata tacttaggcc attttcaact gtcaaaactc
     7741 gaatccgaga accaaatttc accattttcc aaaaatgatg agtgtcgacc aaatggggta
     7801 ctactgtcta atcaggaact tgtgaacaaa ttttcaacct tttccaaata agacgagtgt
     7861 caaccaactt tttccaacca agagatattg ggttgctaca caaatactta atagccattg
     7921 catatttatg catatgcaaa tgcagggtcg tggcgtcaga aagaaacata ggaccctcaa
     7981 catatttaat attttgggag ctatatttga ctatttcata ttagaaaata ataataaaaa
     8041 agtgttggtt ttatatcaaa ttgtaattta cgaaaaactt atgcttttgc gcaatgattt
     8101 ttgtaaagta tctactatgt ttagtgttta cattgattag taggctgccg ttttttttct
     8161 tgtgtattat gtactatata tgaatatgaa catttgtaaa agtgaatctt gtcattttct
     8221 tgttgaaaac atatatagta tgtgcaaaca aagcataggt taatccaata ccacacaaat
     8281 aacacgtcag gtaaatccaa taataaatcg tatgtgcatg tatgtgtatt catgtatgtt
     8341 acatgaatgt ctgaatcagt cagtgtacgt atatgatgta ggtgatgtaa atcttaatgt
     8401 atgagctgtt tcttggacca tggtccacaa tggatattgc tccccaacta cattagtcaa
     8461 tcgactggcc aatttttaat taagataatt aatccaaact accattaaat ataactttga
     8521 ccttttttct attcattttt agatattatt ggaacttacg tagtttacat gcatctcatc
     8581 cctttctttt gctccttgaa agtgggtcca atcacaaaaa atgatcttat attttgtatt
     8641 ttgtatttta aaaactcata attatatagg ttcaaaaatt taattaacat cagtgtatac
     8701 tataattact actctagcca acaagataaa ttcattttga catcagccaa aagataaaaa
     8761 tttggttaaa aactattgga ttagctttta gtatttaata ttttatgtac tgattaaata
     8821 cgaatttaga aatctaggat ataagtgagg gtgtataata agggaggggt ggaccattaa
     8881 tagcgatgtg caattaaaaa ttatgattaa gaatctagga aatttgtaga ttgcttagtt
     8941 atttttatgg cgatcgtcgt gtcaatgtca tggattttga aactttaaat taatctctta
     9001 aattagcacc tacctttgaa ttttatagaa tctttttatt ttatatgttt aattttatag
     9061 aatctaacta gcttattttg agattaaatt gtttagttac ttttataaca gtataaatgt
     9121 ataatgagga cctaagaatg tagtcctgta atgttcttgc tattctactt aatctcatca
     9181 ccaatcaacc atcaaaagaa gctagtacta ataaaacctg caggtattcg aataataatt
     9241 aagctcaaac actatactaa tttatggagg attatatatt caatgaatta ggaacctcat
     9301 gatggacatt attgactgat ataatgtgta tactaattgt gagtatttaa aaaccataca
     9361 aagcatttat atgtccacat atattggaca cacatgcaat caatgttcaa tatgctccac
     9421 acacagaaat aaaaatactc tttctgatca tatgatacat catacatata ctaaaaaaat
     9481 ctaaaatgaa ctataaccac aagcatatat aataacaatg aaatggtaat gtttcttcat
     9541 ttttatttgt tcaaattctt attcggttgt tttttcttac cctacgagaa tccgtgaggt
     9601 caaagggaaa cagtgatttt ttttttgtat tttgtttttt aaattgatga actgtaaaac
     9661 tctctctcta gaaaaatata taagtagtag tatgaatttt ctctcactaa aagcattaat
     9721 ggacctttcg ataatcataa atgcaatgca ccctctctat gcatttcgca ataactcctt
     9781 ttccttctgc cacatcctct tcctcacctc tttctcttct tccctttctc ctaagttcct
     9841 cctccaccaa attctccatt tatttcgtta actatcctcc atttgttttc ttctgaagag
     9901 tgatatattc tacctttctc tggttaaaga aactccctga atccaccggt tatgtcttga
     9961 ccggctataa gcctaatggg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt
    10021 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
    10081 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
    10141 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
    10201 cgcgaagtct ttataccgaa aggtaagtct tactctctct tttttggtct gtatttttaa
    10261 ttttttgaag tatactattt gtactgacgc taataatctt ttttcaggtt gggcaggcca
    10321 gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca
    10381 ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt
    10441 tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac
    10501 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
    10561 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac
    10621 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
    10681 tgactggcag gtacttcatg cttcaacgtg taacttaaga gatactgtgt gaaattttat
    10741 atttccatac atttgcttga cctttgcttt ttgtcaattt ttttcccctt acaggtggtg
    10801 gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga
    10861 caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt
    10921 tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt
    10981 cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg
    11041 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat
    11101 aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt
    11161 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg
    11221 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg
    11281 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg
    11341 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg
    11401 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca
    11461 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg
    11521 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt
    11581 tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa
    11641 gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg
    11701 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt
    11761 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag
    11821 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag
    11881 aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc
    11941 tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg agcttgtcaa
    12001 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    12061 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    12121 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    12181 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    12241 tctatgttac tagatcgacg ctactagagg atgcacatgt gaccgaggga cacgaagtga
    12301 tccgt
//
