LOCUS       sequence-468522-        3545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL2/RE cassette; NS3 systems; 
            lexAO6-pLEU2m-mNeonGreen-tENO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN069
FEATURES             Location/Qualifiers
     source          1..3545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(103..894,895..963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     726..745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaataaa tcgcagccaa gtgagtgaat agatgacgca
     1921 ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg agcactcaca
     1981 catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagcccg tctcagatgc
     2041 acacactggc ttaagatgac aacgggtagt ccatcgttgt aggatactgt atatacaccc
     2101 agtagagtag gtgactactg tatgagcata cagtagtgac aaccacttac tgtatataaa
     2161 tacagtagtg gtcatcgata ctgtatataa aaccagtagg caatcgtttg tactgtatgt
     2221 acatacagta tacctcgcaa ctactgtata taaacacagt aattaacttg taatattcta
     2281 atcaattgac aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt
     2341 cttactttct aacttttctt accttttaca tttcagcaat atatatatat atatttcaag
     2401 gatataccat tctaagatct atggtttcaa aaggggagga ggataatatg gcgagtcttc
     2461 ctgccacgca tgagctgcat atcttcggat ctattaatgg tgtcgacttt gatatggtag
     2521 gacaaggaac cgggaacccc aatgacgggt atgaagagct aaatctaaag agtacaaaag
     2581 gagatttaca gttttctcct tggatacttg ttccccacat tggatatgga tttcatcaat
     2641 atcttcctta tccagacgga atgagcccgt tccaggccgc tatggttgac ggctccgggt
     2701 atcaagtaca ccgtaccatg cagtttgagg atggggcatc cctgaccgta aattacaggt
     2761 acacctacga gggctcacac ataaagggtg aggcgcaggt aaagggtact ggatttcctg
     2821 cggacggccc agtaatgaca aattcactta cagcagcgga ttggtgcagg agcaaaaaga
     2881 cctatcccaa tgacaaaact atcataagta cctttaaatg gtcctatacg acaggtaacg
     2941 ggaagagata taggtcaacg gcaaggacaa cgtatacttt cgcgaagcca atggcagcaa
     3001 actatctgaa gaatcaacct atgtacgtgt tcagaaagac cgagttgaaa cattccaaga
     3061 cagagttaaa tttcaaagaa tggcaaaagg cttttactga cgtaatgggg atggatgaat
     3121 tgtataaggg atcctaactc gagagtgctt ttaactaaga attattagtc ttttctgctt
     3181 attttttcat catagtttag aacactttat attaacgaat agtttatgaa tctatttagg
     3241 tttaaaaatt gatacagttt tataagttac tttttcaaag actcgtgctg tctattgcat
     3301 aatgcactgg aaggggaaaa aaaaggtgca cacgcgtggc tttttcttga atttgcagtt
     3361 tgaaaaatgc tgagcatgag acggaaatct gctcgtcagt ggtgctcaca ctgacgaatc
     3421 atgtacagat cataccgatg actgcctggc gactcacaac taagcaagac agccggaacc
     3481 agcgccggcg aacaccactg catatatggc atatcacaac agtccaacta gtgcactgca
     3541 gtaca
//
