LOCUS       sequence-468523-        4181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConL1/R2 cassette; NS3 systems; 
            pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pWN061
FEATURES             Location/Qualifiers
     source          1..4181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      72..660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     561..580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        979..1645
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1515..1538
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             1655..1675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3185..3976,3977..4045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3808..3827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4046..4150)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      721 ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcccc tgaattcgca
      781 tctagaacgg ggtcatcacg gctcatcatg cgccaaacaa atgtgtgcaa tacacgctcg
      841 gatgactgca tgatgaccgc actgactggg gacagcagat ccacctaagc ctgtgagaga
      901 agcagacacc cgacagatca aggcagttac gtctcaccaa accagatgtc aacacagcta
      961 caacgcagtt cgagtttatc attatcaata ctgccatttc aaagaatacg taaataatta
     1021 atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat tctgctgtaa
     1081 cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg
     1141 ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt taagctggca
     1201 tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca
     1261 taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc
     1321 acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc
     1381 cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg
     1441 gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa
     1501 taagtatata aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct
     1561 taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga acttagtttc
     1621 gaataaacac acataaacaa acaaaagatc tatgcccaag aagaagcgta aagtgccggc
     1681 ggatgctcta gacgatttcg atctggatat gctaccagcc gatgcacttg acgatttcga
     1741 cttagacatg cttccggccg acgctctaga tgacttcgac ttagatatgc tcggttccgg
     1801 cgcgaacaat acaccatttc cgggatattt tgggggcctt gatttattcg actacgactt
     1861 ccttttcggc aacgactttg ctggctcagg tgcgaataat actccttttc caggttattt
     1921 tggaggtttg gatttatttg attatgactt tttgtttggc aatgactttg ctggttcttc
     1981 ttccgatgaa gaggaagcta gagagctaat cgaaagagcg aaggaggcag ccgaaagagc
     2041 tcaagaggcc gctgaaagga ccggtgatcc gagagtgaga gaattggcca gagaactgaa
     2101 gaggttggcc caggaagctg ctgaggaagt caaaagggat cctagcagtt ccgatgtaaa
     2161 cgaagctctt aagctgatag tggaggccat tgaagcagca gtcgacgcgt tagaggcggc
     2221 tgagagaacg ggggaccctg aagtgaggga attagcgcgt gaactagtca ggttggctgt
     2281 agaagccgct gaggaagtac aaagaaaccc gtccagttca gatgttaatg aagcgctgca
     2341 ttctattgtt tatgcgattg aggcggcgat tttcgctctt gaagcggctg agcgtaccgg
     2401 tgatcctgag gtaagagaac tagcaagaga gttagtcaga cttgccgttg aagctgctga
     2461 agaagttcaa cgtaatccat cttcaaggaa cgtagaacat gccttgatga gaatagtgct
     2521 agccatatac ctggcggaag aaaacctacg tgaggccgag gagtctggtg atccagaaaa
     2581 gagagaaaag gcaagggaga gagtcaggga ggctgtggaa agggcagagg aggtccagcg
     2641 tgatccaagt gggtggctga atcatggatc ctaactcgag agcttttgat taagccttct
     2701 agtccaaaaa acacgttttt ttgtcattta tttcattttc ttagaatagt ttagtttatt
     2761 cattttatag tcacgaatgt tttatgattc tatatagggt tgcaaacaag catttttcat
     2821 tttatgttaa aacaatttca ggtttacctt ttattctgct tgtggtgacg cgtgtatccg
     2881 cccgctcttt tggtcaccca tgtatgctgg atgtgagacg ataaatcgca gccaagtgag
     2941 tgaatagatg acgcaccacg gtcagacacg gccacatcgt atctcacagg agcaagcgcg
     3001 ataggagcac tcacacatag tacggtgatc cgctgactcc tttgcccaaa taagacgtga
     3061 gccactagtg cactgcagta cagcggccgc gattatcaaa aaggatcttc acctagatcc
     3121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3301 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     3361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4021 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4081 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4141 gggttccgcg cacatttccc cgaaaagtgc cacctgtcat g
//
