LOCUS       sequence-46854-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Pratt ZL, Zhang J, Sugden B
  TITLE     The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus 
            Can Simultaneously Induce and Inhibit Apoptosis in B Cells.
  JOURNAL   J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.
  PUBMED    22318153
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2012-04"; volume: "86(8)"; pages: "4380-93. Epub 2012 Feb 8"
COMMENT     Sequence Label: pCMMP-MCS-IRES-mRFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..830
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             153..830
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(283..301)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     745..768
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggcctc ctccgaggac gtcatcaagg
      181 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      301 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca
      361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      481 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga
      601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg
      661 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc
      721 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca
      781 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa
//
