LOCUS       sequence-468559-       15500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three 
            inserts (POC1539-POC1541) assembled in POC1519 using the 
            site-selective methylation protection approach.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA
  TITLE     UniClo: scarless hierarchical DNA assembly without sequence 
            constraint.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf548. doi: 
            10.1093/nar/gkaf548.
  PUBMED    40548934
REFERENCE   2  (bases 1 to 15500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf548. doi"; 
            pages: " 10.1093/nar/gkaf548"
COMMENT     Sequence Label: POC1547
FEATURES             Location/Qualifiers
     source          1..15500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4376..4399)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             4410..4432
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    4447..4471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4471..4490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4472..4490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4504..5095)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        5099..5581
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     primer_bind     complement(5602..5618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5602..5618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5615..5637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5626..5642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5650..5656,5657..5674,5675..5680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5695..5716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5833..5850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6004..6592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6084..6103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6763..7554,7555..7623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7386..7405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7624..7728)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7860..7937
                     /gene="lacI"
                     /label=lacI promoter
     CDS             7938..9020
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7957..7976)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      complement(9142..9449)
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     terminator      complement(9474..9521)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     9517..9535
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(9591..9623)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 ggccagctgg tgcaacaaat ggttcacgtt taattaaacg taatttagtt tgtaatgaat
       61 tgtagatagc ttccaattca gagacttctt gaccctttgg tggtttttcg gtttttttat
      121 attctttatg agcgttcatg aaactttgaa cagattcgat ggaatcacca aaatcacgtg
      181 attcaagtga tgcttgttta tcgttaatcc attgaacgag ttcattggct cttttaagat
      241 aatcagatcg tgttgccgcc tcatcaatat tttctgcagt tttcttaatc agcatgacac
      301 cctcacgctc ggccgcatca cggacatgtt cggtaccatc caactggaca ccaataaagt
      361 actggacatc acccttctga tcacgcatag gctgcaagtg aaagaggttc cagaactttt
      421 taccactctt tgtataatta atcagctgta cagtgacctc tgtttggtta tcgatggcat
      481 cacgaatttt gcgcactgtc gcgcgatcgg tttcaggacc ttgaagaaaa cggcagttac
      541 gacccagaat ttcttctcgc gaatattctg tcaactgcaa gaaactatcg gacgcgaaga
      601 taatgggatt atctggcaaa cgtgggtcag taatgacaaa gttcttctca atacgttcaa
      661 gagtagtagc tagcactgcg atctttttct gcctcttcaa ctcgatcctc aaagcctctg
      721 cgtgctcctg ctctgccttt tccagtgcac tccaggtact gtcaatctcg ttgggggtca
      781 atcctgcggg agccacgaag ggctctctct tgatcagcct cagcttggtc tgcagactgt
      841 tatatattgc ctcgagctcg ctcacctcct gtcctttggg gggcttctcc gtcttcttgt
      901 actccttgtg cgcattcata aaggactgca cactctcaat actgtctccg aagtctctac
      961 tctccaaact agcctgcttg tcattgatcc actgcaccaa ctcgttagca cgcttcaagt
     1021 agtcgctacg cgtctgggct tcagctcgat attttatttt gtttttcaat ttaatgactg
     1081 agagcgagca ccactgaact ttcttccagc gactgcagat aagccagtga ttgactctct
     1141 taaccagctc tgctaagtgg tcagggtcgg acttcataat ctgatcaaat tctgcaaact
     1201 tgccaggtct aaaaaatact tttgttaacc caaacttgta gtcaatttca tttaagccca
     1261 gggctttaaa aagagcctta caaaatagtc ttgggtctaa tcttgcaagc ttatccggca
     1321 tatacttctt atacatgttg tagagttcat gaaatgaagc ccgtgatgga aacccgccct
     1381 gcatcaggtc caaaacagac accatacctg aacattgaag ttgagacaaa atctgagcac
     1441 cttcaaagtg gtggcttgtc atctttaaat taggtttgat acaacgaata aagcttgctc
     1501 cagtacttcg aagtttatcc agaagcaaat ttaactgtgt cttaaacttg tttcccacac
     1561 tgatgaagct aagttttcct gctttttgtt tagtatcttt gttgttattt gtggatgatt
     1621 caaataattc ccggatgaat ttatccctgg attcacatat taaggactcg agagacatat
     1681 gcaaagcatc attatttttt tccacgaact gagtagtttc atagcaaact gcccctgcaa
     1741 aatgcctgat aatgaaacct tcgtcatctc gtatgttcct atggattgcc agcttagatt
     1801 ttctaggaat agagagtcgg aaatggtctt tgtgcttctg gtgaactgca gatgtaaagt
     1861 gttgatcact tggctgtgga aggcgatttt cttcatccag aatatccagt attcccacta
     1921 atcttgcttc aattaaatct atacagtcct gattatccac gtaatgtact tcattcacac
     1981 ctaaaccttc cttttgatag agttcttgtt cctccttcag aatcctttca ttaaaaaact
     2041 gttgaagttt ttcattgcaa tagttgatgc aaaattgttc aaaactgtta tgttcaaagt
     2101 actcaaaacc agcaatgtcg aggactccaa taaaatagga tgaggtttca aaaggaaaac
     2161 actgatttac tctgttcact acatgatcaa aaagatggct atagacagtc tttgccaagg
     2221 catcccgggc attgtttgct tgctccactt tcaagggcac ctttataact gttcctttgg
     2281 cgccccctgc tgttgttagc atgactcttg tggttaaact tacacgaaga tcgtcttgat
     2341 ccaaacccag taattctgca caatattcca atgcctgagt agatttattc ttcaggttac
     2401 aaccacctga agtgctgcca gcttcctcaa aatcaatatt tccaagatgt aggacgccag
     2461 ctactacccg gaacagatcg agcttttctt catcatccaa accgattttt ttcatggctg
     2521 tacacattct aataaagtct ccatggtcat ctaacaaagg gtctttcaag gaacctgcct
     2581 taaggtactc aggacttttt cggttctgta aaatctgttt gtcagtttcc ttgttagcaa
     2641 aatatcgagt gcagccccgg tttaaatacc gaaaattatc tggggagctc aaatgaagtc
     2701 tttccctaat atcttcagaa gcaccagcac acaacctata aaagatatga taattccttt
     2761 cctctttgcc ttgaacacag atcctagatt tctctagaag ataatgtgag acaaatcctc
     2821 caacaactga actcttttca ttaaaatgta tttctacaaa tttcccaaat cgactgctat
     2881 tattgttgcg gacagtcttt gcatttccaa aggcttctag gagtgggtta gcttcaacaa
     2941 ttctatcatc aatatcttga ccggttccat aggattcagt cagatatctt agaacaaatt
     3001 ttgtattttc cgttttgcca gctcctgatt ctccagatac aatgatagac tgactcagct
     3061 tgagcacctt catgtctcga aaagccttat cagcaattgc aaagacatga ggtggcatgg
     3121 tcccaagaga ttttccttgg taggacttta ttgtttctga agagtagatt ttaggaatgt
     3181 caaagtatgg gttcacggca atcagaatgt tggccacata tgtataaatt ctgtctttac
     3241 tgtatcgaac tttgatatta tggaggagtg tggcttcatt taaatacatc aatgaacagt
     3301 tatcttccac atctttctta ctgtcctctt ccgcggggaa cacttggttt atgagagcca
     3361 aaaaggtctt gcctttttgg ttcaggggtt caattgttaa gctgtcaggg ccaatatcca
     3421 cgatattgcc cacctgaaat ccatctgtag ggtgtggtgc ccaaacgggt tttccatcct
     3481 cgcttccggc cagcccgggg agccagcgcg cgatctcact gccgataagg tccgggttgt
     3541 cttcctggag caagaacaat cccgggccga tgtccactgt cttgcagttg gggaggcttt
     3601 cggcaagtct cgcggcttcg gccgggggga tcagtacgcc gggtgtgccc cagaacaaca
     3661 acttcgggac aggtgactgg tgcagccagt tcatgtatgc ctcgacgagc gcgacgatgt
     3721 tcgcgggctc accggcgatg ggcagctcgt tggggaatcg ccacagtggc tctcggtcaa
     3781 caggcttgag gaagggctcg cgatagtggt ccatctcgac ctccgtaagc ggacggacga
     3841 cccccatcgg gagcgcaccc tcgatgaaag cgttctgatc gatgatcaac tctcggccga
     3901 cgtcggcggt ccggaaggcc tggaaggttt cacgggcgaa ttctggccat tcgtcccacg
     3961 tcgggatagg ccggatgaat tccatacatg caataccttt gacccgttcc ggattgcgct
     4021 tggcccagtg gaatccgaga gctgagcccc agtcgtggat gaccaggacg acctcttcca
     4081 aacccaaggc ttcgatgaag gcatcgaggt agcggacgtg gtcgtcgaag aaataatcga
     4141 ggtctggttt gtccgatttt cccatcccga tcaggtctgg agcaatgcac cgatgactcg
     4201 gtgctacatg cgggatgatg ttgcgccaca ggtaggacga ggtcgggtta ccgtgcagga
     4261 acagcacagg cgtgccatcc cgcggtccaa catcgacgta gtgcatacgc tcgcccagga
     4321 cttccacata atgggggtcg aaggggaagc ctgtaccgat ttcggaggat ccgcccttat
     4381 cgtcatcgtc cttgtagtcc atgggtatat ctccttctta aagttaaaca aaattatttc
     4441 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttccgcgg
     4501 tcacttggtt gttcacgatc ttgtcgccgc cagtgtcaac ttgcaactga aacaatatcc
     4561 aacatgaacg tcaatttata ctgccctaat ggcgaacacg ataacaatat ttcttttatt
     4621 atgccctcta aaaccaacgc ggttatcgtt tatttattca aattagatat agaacatccg
     4681 ccgacataca atgttaatgc aaaaacgcgt ttggtgagcg gatacgaaaa cagtcggccg
     4741 ataaacatta atctgaggtc gataacaccg tccttgaacg gaacacgagg agcgtacgtg
     4801 atcagctgca ttcgcgcgcc gcgcctttat cgagatttat ttgcatacaa caagtacact
     4861 gcgccgttgg gatttgtggt aacgcgcaca catgcagagc tgcaagtgtg gcacattttg
     4921 tctgtgcgca aaacctttga agccaaaagt acgaggtccg ttacgggcat gctagcgcac
     4981 acggacaatg gacccgacaa attctacgcc aaggatttaa tgataatgtc gggcaacgta
     5041 tccgttcatt ttatcaataa cctacaaaaa tgtcgcgcgc atcacaaaga catcgacgcg
     5101 cgtagaattc tacccgtaaa gcgagtttag ttatgagcca tgtgcaaaac atgacatcag
     5161 cttttatttt tataacaaat gacatcattt cttgattgtg ttttacacgt agaattctac
     5221 tcgtaaagcg agttcagttt tgaaaaacaa atgacatcat ctttttgatt gtgctttaca
     5281 agtagaattc tacccgtaaa tcaagttcgg ttttgaaaaa caaatgagtc atattgtatg
     5341 atatcatatt gcaaaacaaa tgactcatca atcgatcgtg cgttacacgt agaattctac
     5401 tcgtaaagcg agtttatgag ccgtgtgcaa aacatgacat catctcgatt tgaaaaacaa
     5461 atgacatcat ccactgatcg tgcgttacaa gtagaattct actcgtaaag ccagttcggt
     5521 tatgagccgt gtgcaaaaca tgacatcagc ttatgactca tacttgattg tgttttacgc
     5581 gcccgggaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5821 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5881 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5941 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6421 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7741 aaaagtgcca cctgacgtcc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     7801 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
     7861 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     7921 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
     7981 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
     8041 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     8101 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     8161 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
     8221 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
     8281 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
     8341 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
     8401 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
     8461 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
     8521 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
     8581 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
     8641 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
     8701 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
     8761 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     8821 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     8881 gcaatcagct gttgcccgtc agtctggtga aaagaaaaac caccctggcg cccaatacgc
     8941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9001 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca
     9061 ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     9121 cggggcatga ctatcgtgac gtctagattg gttactgggc gatgaaggtt tagtcggcaa
     9181 atcgtccaac gttagtgaca gcgtcagcgg caagttaatg cctatcattt tgttgatagg
     9241 cgcggtcttg tttttaggtt taatatttta ttttatctac agatacatga tgaaaggagg
     9301 gaagggaggt ggtgttggcg cagcaacgtc gccaactccc attgttattt ctatgcaaaa
     9361 ccccacacca acaacggccc ctcgataata aaagacaaaa ataatataaa atatatgtat
     9421 aattaattaa attcaaaata tatgtataac ctgaggatag ttcctccttt cagcaaaaaa
     9481 cccctcaaga cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca
     9541 gcagccaact cagcttcctt tcggctgagg ttaatcactt agtgctcgag atcctcgggg
     9601 tcttccgggg cgagttctgg ctggcttgca cgtgtataca gctgtgcggc cgttaggatc
     9661 ttaatgcaga ccctcacaat tcataatgag tgcggcatcc ttttttggcg aatatgcctt
     9721 ggccttttca gtctcggcta cttgtatgga gcgtagtttc gttttaagtg agtttatctc
     9781 acgattcatg gcttcctgcg attcaatcat gtcctcgcac tcacgctggt atttgcgctt
     9841 ctgcgtcttc tccttctgca attcctcttc ggtttcgtcc aagttgcgct tgagcagttt
     9901 aatgcggcta ttcagcttat ccatttgctc cttgtgctgg tcgacgtgtc tccgttcatc
     9961 ctcgatgttc attgtaagct ccttaatctt cttgtccatc ttccgattgg ccttttgctg
    10021 caacagcctc tccttgccct cattctccag ctgctcttct aggttggcga tcttggcctc
    10081 tagcgtggcg attgtcgcct ttactttagt gcgctgtgct gtctctattt cggcaagctt
    10141 agccttcagc tccttgtttt ggcgttcgag cagagcgcgc ccgttttcgt tcttctgaga
    10201 gttcgatttt tcgtttgcca actcagtggt cagctgctca atctgcagct gcgccttgcg
    10261 gctgcgatcc agcagcacct cagagttgga ctgttcctcc tccaactcct cctcaagagt
    10321 ggcaatgcgg gcttccaatc ggcgcttctc gtcgatcatt aaggatccct tgttggcatt
    10381 gttggcaatt tcttcggcca attcgtcacg ctccgtttcg gcagcacgtc gcgccctctc
    10441 tgagctggcc agatcttcag tcagctgtaa tacttctgcc tccagggcct tgaccttgcg
    10501 ctccgcctcc ttgctcaacg cctgtagctc ttccttggcg gcctttgcct cctcggcgtc
    10561 gcgcagcgcg tccttgactt gtgcttgcag cttcttcgca tgcttcaacg catcttcctt
    10621 caccttgtta tgcatctcca tggtggtttc gatctctttc aggtcgccct ccagtttctt
    10681 ttttgacgcc acagcagccg tgcgctgttt acgttcctcg tctagttcgg tttcaagatc
    10741 ccgcagttgc ttgacaagtc cgcgtctttt ctcctcggca ccctcttcct tagctagcag
    10801 gtcgcgttca aactgggaac gaagggcctg catgttcacc tctaggcgca atttcgcgtc
    10861 ttcggtcagc tgtaggtcgt cctcaagttc ctcgttttgt gctttcagtt cagccagctg
    10921 agactctagc gcccgcttcg ctttctccag ttcgtggacg tttttatcgg ctgtaccctg
    10981 tgtgttggcc aagtcatcga gttcattttg gagggtcttt cgcttgttct caagatcttc
    11041 aattttgtca aaggcttcgt ctagctcgcg ggaaaccgac agcaccttgg tttccttttc
    11101 gcgggcctct cgttctgcag tatcacgctc ttgggcgatc tgttctgata tggctttctc
    11161 ctcggcaaga attttgtcga agtttttttg ttttttctcc aattcgagca ccttcgtacg
    11221 ttgcgcttcc aactcaatgg tggcatcttc aagctccgac tgtatctttt ttttgctttt
    11281 gtcgaggcgg tcgttttggg caataagttc cttaacctgc cgttctaatg cctcgatatc
    11341 cttattgagc cgcttcttcc cctcttccag ttctttggcc agatctgcat cttcctcggc
    11401 cttcttttta atttcttgca tttgggtggt gacttcggcc agttttcgtt cgtaattacg
    11461 tttggcttca tcatcctcct caagctgctc ttgtagagcc tccttctctg actcgatctg
    11521 acgcagcttg gagctgaggc ccaacttttg gcgcgtttcc tcttccaaca gctgctgggc
    11581 ttcagtaagc tgtgattcca tgttgcttgc agactttacg gcggccgagg cttttagttc
    11641 ggcttcctcc agctggtttg ttatgttctc agcttcctgt tgcagttttg tgcacttctc
    11701 ctgaagttcc gagcgggcgc gttcaatctc agccagtttt acctgcaatt cagcaatctg
    11761 cgactctgcc tgcttgcgcc gtcggtcgtt ttcttgccgg gagctgttga cgctgcgcag
    11821 ttcagtggcc aagtcggcgt tttccgcctc caaggtgccc ttggcttttt caaggactgt
    11881 tttggctttt cgtaggtttt caagctgatc gttgatgctg ttgagctcct gcgaatgttt
    11941 gtgtcgcatg tcggccaaaa caccttcgtg gttaacagtc tcctcttcga gagacttttt
    12001 tagggtagcc aactcctgct cgcgtttaga acgcaattct tgctgagcgc tgatgaatcc
    12061 cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt catatgatca
    12121 aatggtttcg ccaaaaatca ataatcagac aacaaaatgt gcgaactcga tattttacac
    12181 gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg
    12241 gcttgccacg ccttacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg
    12301 ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt
    12361 cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat ctgttcgggc
    12421 aatacgatgc ccattgtact tgttgactgg tctgatatcc gtgagcaaaa acggcttatg
    12481 gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga gaaagcgttc
    12541 ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga ccttgcgagc
    12601 attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa agtgccatgg
    12661 tataaatccg ttgagaagct gggttggtac tggttaagtc gagtaagagg aaaagtacaa
    12721 tatgcagacc taggagcgga aaactggaaa cctatcagca acttacatga tatgtcatct
    12781 agtcactcaa agactttagg ctataagagg ctgactaaaa gcaatccaat ctcatgccaa
    12841 attctattgt ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac acggactcat
    12901 tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa aggagccatg gattctagca
    12961 actaacttac ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga
    13021 atgcagattg aagaaacctt ccgagacttg aaaagtcctg cctacggact aggcctacgc
    13081 catagccgaa cgagcagctc agagcgtttt gatatcatgc tgctaatcgc cctgatgctt
    13141 caactaacat gttggcttgc gggcgttcat gctcagaaac aaggttggga caagcacttc
    13201 caggctaaca cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg catggaagtt
    13261 ttgcggcatt ctggctacac aataacaagg gaagacttac tcgtggctgc aaccctacta
    13321 gctcaaaatt tattcacaca tggttacgct ttggggaaat tatgagggga tctctcagtg
    13381 ctgagcggct gtggtgtcca gcgagtccag caactcattc ttgagagcct ctagttcctc
    13441 gctaaggtcg cgtctaacct tctccgcctt ggcacgggcc gccttttcgg cctctaggtc
    13501 ctcttgaatc tcggccagct gcgactccag ctcgcgttga gccttttgtg cggtggcctt
    13561 cgtggccgat tcctcgtcaa tgcgcaacag agtctgggta agttcttcct cgcgtttggc
    13621 cagttgagcc tgcatttcat ctacttgtac gcgccgttcg ttcagctgtt ctttaagatc
    13681 agcaacttca gtctcgatct tccgcttaga tcgatcagac tcctgtcgct gctgctgatc
    13741 cttgtgcaaa cgttcctcga gctccgagat tgtggcttcg tgcttggctt tcagcttggc
    13801 caggtgttta gccttttcct cctcctcagc aagtgtctgg gacagatcgt tagcacgctc
    13861 ttccaatagc ttcttttcct tcagaagctt ttggttctgg tcgtcagtta acgcaagatc
    13921 ttcttcgtac tttttaattt tagcgtcgag ctgcactttc tccaattgta gtttttggcg
    13981 tgcggcctct tcttcttcga gttgctcttc cagatcttga atattaagct caagtttctt
    14041 tttttcgcct ccaagcgcga ggactcgttc ctcttcttct tcgatgcgcg tttcaagctc
    14101 ttgcatcata tcctccagtt cttgtttgcg tgccattagt ctagagcgcg actcctcggc
    14161 ctcggcgcaa agttcgattt cggcttgaag ctgctctgct agagtggtct tctccaccaa
    14221 agcctgctgg tacttgcgct cgtactcctg cgtattcttg gccagagtgt caagcttctc
    14281 gcgtacctgc ttcagctcat cctccttctg gacaagcttc tcttcctgct ttgtaacttc
    14341 caacagggga gaaccggcta ccaatgtcat tttctcgttg atccatgcct cctcttcctc
    14401 aacatttgct acaaactgtt ggtactccag ggactcctcc agacgttgcc cccgagcagc
    14461 tgccagctgt tttaactctt tccagtggtc cacaaactga gccagtcttt gctgtatctc
    14521 ctccttccca attgtgttat catctgaaag cttcttgcca gtgtctagaa caccctggat
    14581 agcaggttca tgggcagcta attctgcttc caagcgctta tgtttcttcc tcaggttctg
    14641 cacaccagtc agatctctgc catagtcctc tgagctcacc aacagtttct tctctttgat
    14701 ccaggactcc tcatcatcca tgtcacggaa gaactgatgc aagcggtgcg actcgttgag
    14761 cttcgcacgg cgggcagctg ccatgctctt gattctctgg aagcgcccat ttatagtttc
    14821 acgtttatcc tttacttggg aggtatcgaa agcactgctg gtcatcaaac tgtcagcctg
    14881 gctgttcagg tccttcagcc gatcctcatg agcagatata tcagcttcca gtaattggtg
    14941 cttcttcaga aggttgttca ctgatgccaa gtccttccca tagtcttcag atgccaacaa
    15001 agcttccacc tctgaaagcc agaaatcaaa gtccttgatg ccggtattga aattctgctg
    15061 tttattttca agttcagcta aaagagtgtt tttgtaggtt tcagcagatg atttaacacc
    15121 agtccattgt tgagcaaaga atgtatcttt acgttcagtg agttctggta caccattgta
    15181 gttgagttca gttaattgag caagaatgct gaggagatca gagtttgatt gaccttccaa
    15241 tgattgacat tcaccatcaa aagcttctaa attctttaat ttagcttgaa cagcagtgat
    15301 actgtcaccg gtttcattgg aaccgaggta gacagatttg gtggtggccc agttttcgag
    15361 tttcttgaga atacgattgt atttttgtaa gagaactgca attttctttt gacgtttgag
    15421 ttcaatacgg agggcttcag catgttcttg ttcagctttc tctaaagcgg accaagtgga
    15481 atcgatttca tttggagtga
//
