LOCUS       sequence-468568-       11831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Hv_HPT:pCST:Gus_introns
FEATURES             Location/Qualifiers
     source          1..11831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      130..840
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1135..1926
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2756..2775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3191..4339
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4485..4509
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5596..5941
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5884..5904
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          6371..6560
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     polyA_signal    7282..7456
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      11531..11783
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
       61 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
      121 tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc
      181 cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg
      241 ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga
      301 cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg
      361 atttcggccg gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta
      421 cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca
      481 cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag
      541 tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc
      601 cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc
      661 ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc
      721 gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc
      781 tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag
      841 cgctggccaa ttcccgaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
      901 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      961 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     1021 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     1081 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     1141 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     1201 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     1261 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     1321 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     1381 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     1441 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     1501 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     1561 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     1621 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     1681 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     1741 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     1801 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     1861 gcagatcagt tggaagaatt tgtccattac gtgaaaggcg agatcaccaa ggtagtcggc
     1921 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     1981 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     2041 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgctgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc agatcctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga
     2941 cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa
     3001 ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca
     3061 gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc
     3121 aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca
     3181 aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc
     3241 ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg
     3301 cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga
     3361 cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg
     3421 tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac
     3481 tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc
     3541 caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt
     3601 gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc
     3661 tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat
     3721 tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga
     3781 tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc
     3841 gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta
     3901 ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc
     3961 cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg
     4021 tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc
     4081 tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg
     4141 acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt
     4201 ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga
     4261 agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct
     4321 ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagcccc
     4381 tgctcggatc tgttggaccg gacagtagtc atggttgatg ggctgcctgt atcgagtggt
     4441 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
     4501 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
     4561 tggggttgaa cactctgtgg gtctcatgcc gaattcggat ccggaggaat tccaatccca
     4621 caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac
     4681 cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg
     4741 ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact
     4801 attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg
     4861 aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca
     4921 agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat
     4981 ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt
     5041 tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat
     5101 gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
     5161 tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac
     5221 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca
     5281 gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
     5341 cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa
     5401 tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa
     5461 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     5521 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     5581 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     5641 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     5701 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
     5761 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
     5821 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     5881 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     5941 acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     6001 cattacaatt acatttacaa ttatcgatac aatgaaaaag cctgaactca ccgcgacgtc
     6061 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     6121 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     6181 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     6241 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     6301 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     6361 tgttctgcag gtaaatttct agtttttctc cttcattttc ttggttagga cccttttctc
     6421 tttttatttt tttgagcttt gatctttctt taaactgatc tattttttaa ttgattggtt
     6481 atggtgtaaa tattacatag ctttaactga taatctgatt actttatttc gtgtgtctat
     6541 gatgatgatg ataactgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6601 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6661 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6721 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6781 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6841 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6901 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     6961 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     7021 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7081 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7141 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7201 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataggct tctctagcta
     7261 gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg
     7321 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     7381 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     7441 tactaaaatc cagatcgctg caagaattca agcttggagg gagccgtcgc ttttgcacac
     7501 agacggccgt gctcgtaagc agcccagaca cgcaaacacg cctagttgca gcccgggagc
     7561 gcagccgggc tgctgggcca gcgtcgcaaa cacgccctaa atcagagagg gaaagcccca
     7621 catatataac tagatgtctt attcaaatat cacctctaag gattacttat cctggtaaat
     7681 gagacgattc caggaagatt tgggtttcca aactgtccct tagaagggcc actctactgt
     7741 cagccactct tgtctaggct actttatact ggatacaaac tcattacgct gaagcccttt
     7801 atattggtca atatcatgag atctccagac gccgccgtca ctcgatcgag atagatatag
     7861 cctctttccg tccacgtaca tccgctgctg gccttcagat ctctatcaag tgacgcggca
     7921 aacaggacgc agcaccgggg aactacgacg agtactatat agcagataaa cgcatgctct
     7981 tggccgttgt tagagccctt gattgcatgg cagtgtcgat cgcccaactc cagcctgagt
     8041 tctcagaacc tccccggccg gcccagtgtc cagcgtgctg ctcacaatct acgggatcag
     8101 gcgatcagct atagtagccc ttcacatcgg ggtgcattta tgtgtcttaa tttgctcagc
     8161 agtcaaccat ccatcatgca cttgcacttg ttcatcgcca tggttggcag ggaaccaata
     8221 ttaggcccgg aatttctctt tcgtcgatgg cccagctctt ttttccagtg attcattttc
     8281 acgcacgcct cagagataaa tcttgtgttt attcactttg tcaatttggt ttgatcaaaa
     8341 ttacacttgg atttacatcg atatcattgt tttgtgcgat agttgcttaa gtaaccatat
     8401 agaagctcac atttgtaatt aagctgtatt catttcccta gtgtgattaa gtagttgcta
     8461 gattgtgtgc ttgagtaaag tcaacctaga gatttagcag ctagatttgt tcatgctgtt
     8521 tagtctaatt tctttgtaaa tggaaggtgt actgtacaca cttggtgatc taatgtgtgc
     8581 atgctgttta atctgactag tatatgatga gcaatgtcta tagtagtata tgcttgttta
     8641 tgttacataa tttggctaat atatcagcaa tactcatgct aatacttatt gtgttcatta
     8701 ttattattat tattattatt tcaatctaac taatatatga gcagtgttcg tacctcggac
     8761 caatacttga gtttgtcatg tttcatattc tgattttacc cacttttgaa aaaacaacaa
     8821 cccctgccac cgctttagtg ctagctgtga caaacacacg aatgtgaata catggtcagt
     8881 ataccttcta tgcatggtct atgcatggtc gaggtggtgg accaggacgc atcctgatgc
     8941 caaactctga cctctgtggt gacaaatgag aaaaccggcg tctcatctga tggtgctgga
     9001 agccgcactg ctcactgccc cggcgcacac cacgtccacg cgtctgcact actactccgg
     9061 ccgggcactc gtcgtcctca gtggtggtca agataagaac gcaatcaatg aatcaatggg
     9121 tgcaggcagc aggcagcagg ctttggcttg acacaccagc gagaagatgc cctcctccct
     9181 ccaactcttc ctatatatat acacatgaaa ccgtcatcca tgtatccatc catccacaca
     9241 cacaaacaga caagcataca acaccgttgc catttagctg ttcttgctag gcagttgcgc
     9301 gcttacattg agccgcagct agctgtctac tacggcacaa ctatctcctg cgcgcgtgct
     9361 cgatcgatct gaaggccaga cctgtgcctg tccaattttg ccttgttcta cccaccacac
     9421 cagtagtcga gaaggttcca tataagcgca agcagacagc agcaaaagag ctaaggagag
     9481 tctgagacaa ggttcgaaca gaatgggtca gtcccttatg ttacgtcctg tagaaacccc
     9541 aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg
     9601 tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc
     9661 aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg
     9721 gtatcagcgc gaagtcttta taccgaaagg taagtcttac tctctctttt ttggtctgta
     9781 tttttaattt tttgaagtat actatttgta ctgacgctaa taatcttttt tcaggttggg
     9841 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
     9901 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
     9961 cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac gaactgaact
    10021 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
    10081 acttccatga tttctttaac tatgccggaa tccatcgcag cgtaatgctc tacaccacgc
    10141 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
    10201 cgtctgttga ctggcaggta cttcatgctt caacgtgtaa cttaagagat actgtgtgaa
    10261 attttatatt tccatacatt tgcttgacct ttgctttttg tcaatttttt tccccttaca
    10321 ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc
    10381 aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg
    10441 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
    10501 cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgattaacca
    10561 caaaccgttc tactttactg gctttggtcg tcatgaagat gcggacttgc gtggcaaagg
    10621 attcgataac gtgctgatgg tgcacgacca cgcattaatg gactggattg gggccaactc
    10681 ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg
    10741 catcgtggtg attgatgaaa ctgctgctgt cggctttaac ctctctttag gcattggttt
    10801 cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaactca
    10861 gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt
    10921 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
    10981 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
    11041 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
    11101 gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact
    11161 ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
    11221 cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta
    11281 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg
    11341 tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
    11401 taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca
    11461 aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgagc
    11521 ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    11581 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
    11641 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    11701 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    11761 ggtgtcatct atgttactag atcgacgcta ctagaggatg cacatgtgac cgagggacac
    11821 gaagtgatcc g
//
