LOCUS       sequence-468570-       12402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Hv_HPT:pGstA+WIR1a:Gus_introns
FEATURES             Location/Qualifiers
     source          1..12402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    38..62
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      150..860
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1155..1946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2287..2875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2776..2795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3211..4359
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4505..4529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5616..5961
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5904..5924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          6391..6580
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     polyA_signal    7302..7476
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     7500..7516
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7500..7516
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      12122..12374
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
       61 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
      121 tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc
      181 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
      241 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      301 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
      361 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
      421 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
      481 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
      541 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
      601 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
      661 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
      721 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
      781 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
      841 cctcacccca aaaatggcag cgctggccaa ttcccgaggc acgaacccag tggacataag
      901 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      961 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1021 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1081 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1141 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1201 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1261 ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg
     1321 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1381 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1441 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1501 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1561 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1621 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1681 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1741 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1801 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1861 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtgaaaggcg
     1921 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     1981 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     2041 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     2101 tgggagatat atcatgctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc
     2941 gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc
     3001 cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc
     3061 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
     3121 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
     3181 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
     3241 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
     3301 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
     3361 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
     3421 gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg
     3481 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
     3541 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
     3601 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
     3661 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
     3721 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
     3781 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
     3841 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
     3901 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
     3961 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
     4021 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
     4081 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
     4141 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
     4201 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
     4261 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
     4321 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
     4381 gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc atggttgatg
     4441 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     4501 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     4561 tgcggacgtt tttaatgtac tggggttgaa cactctgtgg gtctcatgcc gaattcggat
     4621 ccggaggaat tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag
     4681 cagaatcggg tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg
     4741 ccggtatata cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg
     4801 cacacaagaa atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa
     4861 gtcagcaaac agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga
     4921 gctttgctaa ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa
     4981 ccaaaaggcc cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa
     5041 tggacgattt cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca
     5101 ctatgttcac cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc
     5161 cacagatggt tagagaggcc tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca
     5221 atctccagga gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     5281 ctaattgcat caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag
     5341 tatggacgat tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta
     5401 aaaaggtagt tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat
     5461 ctaacagaac tcgccgtcaa gactggcgaa cagttcatac agagtctttt acgactcaat
     5521 gacaagaaga aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat
     5581 gtcaaagata cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt
     5641 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
     5701 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
     5761 gatctctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     5821 aaagaagagg ttccaaccac gtctacaaag caagtggatt gatgtgacat ctccactgac
     5881 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     5941 tcatttcatt tggagaggac acgctcgagt ataagagctc atttttacaa caattaccaa
     6001 caacaacaaa caacaaacaa cattacaatt acatttacaa ttatcgatac aatgaaaaag
     6061 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     6121 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     6181 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6241 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6301 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6361 cctgaaaccg aactgcccgc tgttctgcag gtaaatttct agtttttctc cttcattttc
     6421 ttggttagga cccttttctc tttttatttt tttgagcttt gatctttctt taaactgatc
     6481 tattttttaa ttgattggtt atggtgtaaa tattacatag ctttaactga taatctgatt
     6541 actttatttc gtgtgtctat gatgatgatg ataactgcag ccggtcgcgg aggccatgga
     6601 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     6661 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     6721 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     6781 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     6841 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     6901 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     6961 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7021 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     7081 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     7141 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     7201 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     7261 ggaataggct tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     7321 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     7381 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     7441 ttcctaaaac caaaatccag tactaaaatc cagatcgctg caagaattca agcttggagt
     7501 ctagaactag tggatccccg acgccgaagt ggagccgaca gcccccaggt cccaagccct
     7561 cggcagacta gatcactagc cctggatcgg cgaggtgact ggatgacgag cagcacctgg
     7621 tctggcgggt gttgggcgag tagaaccagg ggcgatggcg acgcgctgac cttctcccct
     7681 caccggcgat ctgctccttc tgggtggggg tcgccggctg acgttctgtt gcggggtggg
     7741 ggtcgccggc tggcgttctg ctgcggggtg ggagtcgccg accggcgtgc tgctgctagg
     7801 acaatcggtg aggccagtta ggtgctagcc gatcgattgg cgaagagatc cgagtcctgg
     7861 ggagatcagt gaggccaggt gctatttggc ctatcaattg gccaggttct gggaacgggg
     7921 cgtggcgtga tcaacgaggt gctaggctgc tagctaggga actggatcct ggaacgtgga
     7981 ggaggcaagt ccggtatgct aagtacttta actttccttc ttcacatcca cctgattcag
     8041 attattttga tctaaattaa cttgcaaaaa atatatgtgt gatatccatc tactataatt
     8101 gcttacaatc aaaattatat gtgatttttt ttagtttaga agatttatat gcacagtaaa
     8161 tctgaatgtt cttcacatgc atgatttagt ttaactttaa agagttatac taactagtct
     8221 tgataaagag atcttttgga gcaacaccaa acctcgtgag gtgttttgcc tacggaaagg
     8281 ttgtgctatg taatgattat tattaggatc aaagttgtag gataaacgta aaaccttctc
     8341 gatgtatctt ttatacaaca ttgtagttta gttatatatg gagagagtga tttaacactt
     8401 tgtgtttaag agtagaataa gttattccac actctagcca aacgaactat ttggcaaata
     8461 tctcgctagc tggtgagagc cagagccgtg gaaagtctgt cttgctatta aggcacaagc
     8521 atcaaacagg aacatttaga gccatggaaa agtgatgtgt cgcctaccaa tgggccaact
     8581 gctagcgatg taataatagc atccaagttg attttttata gaacatgcaa ggcgttggca
     8641 agtgggaaaa tgattgatcg ctggcaagct taactctcgg aacttatagc attcaactga
     8701 atcagaacaa agattaaaaa aaaatacatt tccatcgata gtgaaaaatt attcaattga
     8761 gtgacaacga aaatcatatt ggaatgtaca tttacttgtt gattttaaat tagaggcatt
     8821 tttctacctt ttttagttaa taagatatgc atatacccac ccttagtgtt ttcgagacaa
     8881 cgagagggca cattgctttt ggtgctacca tctctctcaa gcctcaaata agttgtgcgg
     8941 acacgattat cttcccgcgt tggaatatcg tggcctggta gagctagcga aaaatcttcc
     9001 atgttggaat atgtcggcag ccggatagcc gccatgcatg taaagtctct tttaccttta
     9061 cacttgctca agtgacactg tatgtcgcct accacttgct aaatcaatgg gccaactgct
     9121 agcgacgtaa tagtagcaag ttgatttaca gtgttttgct acagttctct gactttgttt
     9181 cttcatttta gactagctga ctactgtcgc ttacctgcct tcccttctcc acgttagagg
     9241 atccagttct gatattgaca cctcgacgat gggaggaagg gcgcgatcga tgtggagtaa
     9301 tttgaatttc aaatctatct atctggggta tattggtcct tcaccgatgt ttggggggct
     9361 gtcggaaatt ggttccgcga tctacaaaag tgaatggagg gagtagttgt ttctccaatc
     9421 cgtaccaacg cacgtgtttc taactagtac ttacttcctt cgcaccacaa tatggaatag
     9481 agggagtatc gataaactaa caaagatgat tacttacccg gtttaaatga ttcaagagct
     9541 catttaattt ggcactcatc atttcatata tcttttttgg tagaaatgaa ataaagcaga
     9601 tctagacact agctaaaaag tcgatgtagc cttgttattt ccttgggcca cgcgggccgg
     9661 gtgtggtgct ccctgctctg tgtataaatg gagatcaaca tccaaggcct cctcccacac
     9721 acacacgcta cagagcagag cagagtcttg ctccagtatc tgccctctcc tgcctgcctg
     9781 tagagcatcc atcacgtgaa gttcacggac aaactacgta cacaggcagc tagctctcga
     9841 aacctcgctc gaaacgcacc tgcagatcgc tctcttcgtc gtcgtcgccg cgatcatcat
     9901 caacagctcc gtctgccttg gagccacggc cgtccacgac gccgccgcct caggtcagtc
     9961 gtcggacggt gtccgttcat ttcctcccca tttttgtaat tgattaactt gttatacatg
    10021 ctgacctcga cctgctgaat aacgtccgtc catggtttcc cgtccaggca ccccgggcca
    10081 ctgtccacac gaaatgggtc agtcccttat gttacgtcct gtagaaaccc caacccgtga
    10141 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
    10201 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
    10261 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
    10321 cgaagtcttt ataccgaaag gtaagtctta ctctctcttt tttggtctgt atttttaatt
    10381 ttttgaagta tactatttgt actgacgcta ataatctttt ttcaggttgg gcaggccagc
    10441 gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg
    10501 aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta
    10561 ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta
    10621 tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg
    10681 atttctttaa ctatgccgga atccatcgca gcgtaatgct ctacaccacg ccgaacacct
    10741 gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg
    10801 actggcaggt acttcatgct tcaacgtgta acttaagaga tactgtgtga aattttatat
    10861 ttccatacat ttgcttgacc tttgcttttt gtcaattttt ttccccttac aggtggtggc
    10921 caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca
    10981 aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta
    11041 tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg
    11101 cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt
    11161 ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa
    11221 cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac
    11281 ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt
    11341 gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg
    11401 caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca
    11461 cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg
    11521 gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact
    11581 ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt
    11641 ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta
    11701 ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga
    11761 acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga
    11821 tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc
    11881 atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt
    11941 atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa
    12001 agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg
    12061 gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgag cttgtcaagc
    12121 agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
    12181 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
    12241 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
    12301 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
    12361 tatgttacta gatcgacgct actagaggat gcacatgtga cc
//
