LOCUS       sequence-468572-       10709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Hv_HPT:pSNAC:Gus_introns
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      148..858
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1153..1944
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2285..2873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2774..2793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3209..4357
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4503..4527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5614..5959
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5902..5922
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          6389..6578
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     polyA_signal    7300..7474
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      10427..10679
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgccagccgc ctttgcgacg ctcaccgggc tggttgccct
      181 cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc
      241 gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
      301 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
      361 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
      421 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
      481 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
      541 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
      601 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
      661 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
      721 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
      781 ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc
      841 tcaccccaaa aatggcagcg ctggccaatt cccgaggcac gaacccagtg gacataagcc
      901 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
      961 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     1021 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1081 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1141 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1201 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1261 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1321 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1381 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1441 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1501 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1561 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1621 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1681 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1741 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1801 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     1861 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt gaaaggcgag
     1921 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     1981 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     2041 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     2101 ggagatatat catgctgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2881 gcaacgcggc ctttttacgg ttcctggcag atcctagatg tggcgcaacg atgccggcga
     2941 caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca
     3001 cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa
     3061 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
     3121 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
     3181 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
     3241 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
     3301 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
     3361 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
     3421 cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca ggtttggcga agtcgatgac
     3481 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
     3541 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
     3601 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
     3661 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
     3721 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
     3781 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
     3841 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
     3901 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
     3961 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
     4021 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
     4081 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
     4141 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
     4201 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
     4261 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
     4321 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
     4381 tgggggttca gcagcccctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg
     4441 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     4501 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     4561 cggacgtttt taatgtactg gggttgaaca ctctgtgggt ctcatgccga attcggatcc
     4621 ggaggaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     4681 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     4741 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     4801 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     4861 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     4921 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     4981 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     5041 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     5101 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     5161 cagatggtta gagaggccta cgcagcaagt ctcatcaaga cgatctaccc gagtaacaat
     5221 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     5281 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     5341 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     5401 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     5461 aacagaactc gccgtcaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     5521 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     5581 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
     5641 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     5701 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     5761 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     5821 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
     5881 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     5941 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
     6001 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tgaaaaagcc
     6061 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     6121 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6181 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     6241 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     6301 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     6361 tgaaaccgaa ctgcccgctg ttctgcaggt aaatttctag tttttctcct tcattttctt
     6421 ggttaggacc cttttctctt tttatttttt tgagctttga tctttcttta aactgatcta
     6481 ttttttaatt gattggttat ggtgtaaata ttacatagct ttaactgata atctgattac
     6541 tttatttcgt gtgtctatga tgatgatgat aactgcagcc ggtcgcggag gccatggatg
     6601 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     6661 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     6721 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     6781 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     6841 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     6901 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     6961 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     7021 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     7081 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     7141 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     7201 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     7261 aataggcttc tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga
     7321 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     7381 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     7441 cctaaaacca aaatccagta ctaaaatcca gatcgctgca agaattcaag cttggagtta
     7501 ctgcaaggtg tctgatttgg agtcaaaatt tcggcctagc caaccaacta acttgaaaaa
     7561 tgattttata ttacaagatg gagtactatc atttataaaa gttgccttta agggacaatt
     7621 aatattggac ggagggatat gagctgtctt taaaagaaaa gatagaatga aaaacaaaga
     7681 gcacatgata aaagcacggt aaaaataaat atcagtcgtt ttcttcttga cattgttttt
     7741 gtttcttttg aggctgcttg ccacatactc ccttagtaaa aaatactgta atataaaagg
     7801 cgtttataga tagctaaagt acggcatgtg gtgataaaaa atatatatca gtcgatgcca
     7861 tatattttct ccaattttgt ttcgtgggag atcctgtaga gcagctgctg ccacgacatg
     7921 cctccgtttc aaaaaataaa taaaaagaaa ggcaggtgta gacccgagca tcagccacgc
     7981 agaggcgtat ccacagtgct cctccgatcc cacctatcgg cacgatccgc ggtgtccaca
     8041 cacgcgcatg ccgaccagct tcacgccgca ccgcacaacc cgcacggcca gctgaagtcg
     8101 gccatgacgc aacccccgtg ccacccggcc gcgccttccc cgaaaccccc cacacacgtg
     8161 cccttttcct ccccaagaaa cccctccacg cagccgccgc ccccctaccc ccgtccgaac
     8221 cttccagacc actcacgcac cccccctccc ctatttatcc ctcccccatc cccccattta
     8281 ccaccaccat caccacacaa cccaactcct ctcactcccc aacaatctca aacaacagct
     8341 cgaggaatca acagcagcga ctactctgtt cgttgaacca acaatcaaca gacggacaat
     8401 gggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga
     8461 cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc gttggtggga
     8521 aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc
     8581 cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag tctttatacc
     8641 gaaaggtaag tcttactctc tcttttttgg tctgtatttt taattttttg aagtatacta
     8701 tttgtactga cgctaataat cttttttcag gttgggcagg ccagcgtatc gtgctgcgtt
     8761 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc
     8821 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg
     8881 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg
     8941 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg
     9001 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
     9061 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtacttc
     9121 atgcttcaac gtgtaactta agagatactg tgtgaaattt tatatttcca tacatttgct
     9181 tgacctttgc tttttgtcaa tttttttccc cttacaggtg gtggccaatg gtgatgtcag
     9241 cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca ctagcgggac
     9301 tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct atgaactgtg
     9361 cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg gcatccggtc
     9421 agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact ttactggctt
     9481 tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc tgatggtgca
     9541 cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc attaccctta
     9601 cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg atgaaactgc
     9661 tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga
     9721 actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac aggcgattaa
     9781 agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta ttgccaacga
     9841 accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg aagcaacgcg
     9901 taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg acgctcacac
     9961 cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg gatggtatgt
    10021 ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc tggcctggca
    10081 ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt tagccgggct
    10141 gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc tggatatgta
    10201 tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga atttcgccga
    10261 ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga tcttcactcg
    10321 cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg gcatgaactt
    10381 cggtgaaaaa ccgcagcagg gaggcaaaca atgagcttgt caagcagatc gttcaaacat
    10441 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
    10501 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
    10561 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
    10621 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
    10681 acgctactag aggatgcaca tgtgaccga
//
