LOCUS       sequence-468576-       13252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Evaluating guard cell specific promotors in Barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Nguyen TH, Krasauskas J, Nguyen TB, Noureen A, Smedley M, Christie 
            JM, Harwood W, Blatt MR, Hundleby P
  TITLE     A Promoter Collection for Cell-Targeted Analysis Within the Stomatal
            Complex.
  JOURNAL   Plant Direct. 2025 Feb 12;9(2):e70045. doi: 10.1002/pld3.70045. 
            eCollection 2025 Feb.
  PUBMED    39943924
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2025-02-12"; volume: "9(2):e70045. doi"; pages: " 
            10.1002/pld3.70045. eCollection 2025 Feb"
COMMENT     Sequence Label: Hv_HPT:pATML:Gus_introns
FEATURES             Location/Qualifiers
     source          1..13252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    38..62
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      150..860
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1155..1946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2287..2875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2776..2795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3211..4359
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4505..4529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5616..5961
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5904..5924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          6391..6580
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     polyA_signal    7302..7476
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      12972..13224
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
       61 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
      121 tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc
      181 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
      241 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
      301 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
      361 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
      421 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
      481 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
      541 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
      601 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
      661 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
      721 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
      781 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
      841 cctcacccca aaaatggcag cgctggccaa ttcccgaggc acgaacccag tggacataag
      901 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      961 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1021 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1081 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1141 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1201 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1261 ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg
     1321 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1381 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1441 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1501 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1561 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1621 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1681 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1741 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1801 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1861 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtgaaaggcg
     1921 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     1981 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     2041 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     2101 tgggagatat atcatgctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2881 cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc
     2941 gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc
     3001 cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc
     3061 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
     3121 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
     3181 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
     3241 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
     3301 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
     3361 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
     3421 gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg
     3481 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
     3541 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
     3601 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
     3661 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
     3721 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
     3781 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
     3841 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
     3901 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
     3961 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
     4021 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
     4081 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
     4141 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
     4201 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
     4261 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
     4321 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
     4381 gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc atggttgatg
     4441 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     4501 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     4561 tgcggacgtt tttaatgtac tggggttgaa cactctgtgg gtctcatgcc gaattcggat
     4621 ccggaggaat tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag
     4681 cagaatcggg tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg
     4741 ccggtatata cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg
     4801 cacacaagaa atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa
     4861 gtcagcaaac agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga
     4921 gctttgctaa ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa
     4981 ccaaaaggcc cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa
     5041 tggacgattt cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca
     5101 ctatgttcac cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc
     5161 cacagatggt tagagaggcc tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca
     5221 atctccagga gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     5281 ctaattgcat caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag
     5341 tatggacgat tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta
     5401 aaaaggtagt tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat
     5461 ctaacagaac tcgccgtcaa gactggcgaa cagttcatac agagtctttt acgactcaat
     5521 gacaagaaga aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat
     5581 gtcaaagata cagtctcaga agatcaaagg gctattgaga cttttcaaca aaggataatt
     5641 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
     5701 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
     5761 gatctctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     5821 aaagaagagg ttccaaccac gtctacaaag caagtggatt gatgtgacat ctccactgac
     5881 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     5941 tcatttcatt tggagaggac acgctcgagt ataagagctc atttttacaa caattaccaa
     6001 caacaacaaa caacaaacaa cattacaatt acatttacaa ttatcgatac aatgaaaaag
     6061 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     6121 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     6181 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6241 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6301 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6361 cctgaaaccg aactgcccgc tgttctgcag gtaaatttct agtttttctc cttcattttc
     6421 ttggttagga cccttttctc tttttatttt tttgagcttt gatctttctt taaactgatc
     6481 tattttttaa ttgattggtt atggtgtaaa tattacatag ctttaactga taatctgatt
     6541 actttatttc gtgtgtctat gatgatgatg ataactgcag ccggtcgcgg aggccatgga
     6601 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     6661 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     6721 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     6781 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     6841 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     6901 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     6961 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7021 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     7081 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     7141 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     7201 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     7261 ggaataggct tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     7321 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     7381 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     7441 ttcctaaaac caaaatccag tactaaaatc cagatcgctg caagaattca agcttggagt
     7501 tttacattga ttctgaactg tacccaattt ttttaaagct tatcaaagaa aaaacaagaa
     7561 caaaacgatg catagtttct aaaatgtgct aaaattcaga aactgaaaca tgattcattg
     7621 tctgaaactt tgtttcaaat tactgaaaat aatcattcac tggaccaaaa caaataaata
     7681 aaataaaatc gaatttctga atttggaaat tggtttttgg tttttaattt taaacaaaac
     7741 aaaaacgaaa tttgaaggca ataaatgagt tagttggtag gcagaagtca ctcgttccca
     7801 ctagctatta ttattagaag aaacgtcccc acaactccaa ggcgtttcag ttcctttaat
     7861 ttactgaatt accctcctca tatctataaa aaatcacctc ttgtaccaat gccccattta
     7921 cacatcctgt cgtttatttc tagactaagt ggactacatg tcggttattt gattcgcacc
     7981 atgcgtattt ggattatcgc taacacaccc cttcaaacaa tacgcttaac tcgtattaca
     8041 aaatttcaag tgatgaatta tctatgtata agatatagat aggaacaact aagcatcgag
     8101 aaatttgtat ataaatcaac tagacttata tatatttcga tacagaattt atacgcatta
     8161 tatcaaatta attagtaatt gtttcctcta cgtgagttta attaacaatg ataagctaca
     8221 ttgagtgtat cagttctaaa actttatagt atgctacaat caatttttct aagtaacaac
     8281 ttcaagcaag gaatcacaca cacacagtgg tacataataa acttgatttt aatatcatat
     8341 gatcagcatc attaacggaa taagttaagt aattcgtcat ccatactact aagtcatatt
     8401 aaaatcataa tcaaacttaa aagccgatta gaaagagagc aaatatatct aaaaattcac
     8461 gaggaatacg acaaatgcaa ggaaacacag ctagtattat taaacttaat agatattgga
     8521 tgaatgactg cataatatat atcacattaa aagtggacat aaatttgcat atgtgtaatg
     8581 tacctctcca caattaatcg cggaccattt attttactat tacaagtcaa gtaactttat
     8641 attgttgatc cataattctt ttcgaacata aaatcatata cttaggccat tttcaactgt
     8701 caaaactcga atccgagaac caaatttcac cattttccaa aaatgatgag tgtcgaccaa
     8761 atggggtact actgtctaat caggaacttg tgaacaaatt ttcaaccttt tccaaataag
     8821 acgagtgtca accaactttt tccaaccaag agatattggg ttgctacaca aatacttaat
     8881 agccattgca tatttatgca tatgcaaatg cagggtcgtg gcgtcagaaa gaaacatagg
     8941 accctcaaca tatttaatat tttgggagct atatttgact atttcatatt agaaaataat
     9001 aataaaaaag tgttggtttt atatcaaatt gtaatttacg aaaaacttat gcttttgcgc
     9061 aatgattttt gtaaagtatc tactatgttt agtgtttaca ttgattagta ggctgccgtt
     9121 ttttttcttg tgtattatgt actatatatg aatatgaaca tttgtaaaag tgaatcttgt
     9181 cattttcttg ttgaaaacat atatagtatg tgcaaacaaa gcataggtta atccaatacc
     9241 acacaaataa cacgtcaggt aaatccaata ataaatcgta tgtgcatgta tgtgtattca
     9301 tgtatgttac atgaatgtct gaatcagtca gtgtacgtat atgatgtagg tgatgtaaat
     9361 cttaatgtat gagctgtttc ttggaccatg gtccacaatg gatattgctc cccaactaca
     9421 ttagtcaatc gactggccaa tttttaatta agataattaa tccaaactac cattaaatat
     9481 aactttgacc ttttttctat tcatttttag atattattgg aacttacgta gtttacatgc
     9541 atctcatccc tttcttttgc tccttgaaag tgggtccaat cacaaaaaat gatcttatat
     9601 tttgtatttt gtattttaaa aactcataat tatataggtt caaaaattta attaacatca
     9661 gtgtatacta taattactac tctagccaac aagataaatt cattttgaca tcagccaaaa
     9721 gataaaaatt tggttaaaaa ctattggatt agcttttagt atttaatatt ttatgtactg
     9781 attaaatacg aatttagaaa tctaggatat aagtgagggt gtataataag ggaggggtgg
     9841 accattaata gcgatgtgca attaaaaatt atgattaaga atctaggaaa tttgtagatt
     9901 gcttagttat ttttatggcg atcgtcgtgt caatgtcatg gattttgaaa ctttaaatta
     9961 atctcttaaa ttagcaccta cctttgaatt ttatagaatc tttttatttt atatgtttaa
    10021 ttttatagaa tctaactagc ttattttgag attaaattgt ttagttactt ttataacagt
    10081 ataaatgtat aatgaggacc taagaatgta gtcctgtaat gttcttgcta ttctacttaa
    10141 tctcatcacc aatcaaccat caaaagaagc tagtactaat aaaacctgca ggtattcgaa
    10201 taataattaa gctcaaacac tatactaatt tatggaggat tatatattca atgaattagg
    10261 aacctcatga tggacattat tgactgatat aatgtgtata ctaattgtga gtatttaaaa
    10321 accatacaaa gcatttatat gtccacatat attggacaca catgcaatca atgttcaata
    10381 tgctccacac acagaaataa aaatactctt tctgatcata tgatacatca tacatatact
    10441 aaaaaaatct aaaatgaact ataaccacaa gcatatataa taacaatgaa atggtaatgt
    10501 ttcttcattt ttatttgttc aaattcttat tcggttgttt tttcttaccc tacgagaatc
    10561 cgtgaggtca aagggaaaca gtgatttttt ttttgtattt tgttttttaa attgatgaac
    10621 tgtaaaactc tctctctaga aaaatatata agtagtagta tgaattttct ctcactaaaa
    10681 gcattaatgg acctttcgat aatcataaat gcaatgcacc ctctctatgc atttcgcaat
    10741 aactcctttt ccttctgcca catcctcttc ctcacctctt tctcttcttc cctttctcct
    10801 aagttcctcc tccaccaaat tctccattta tttcgttaac tatcctccat ttgttttctt
    10861 ctgaagagtg atatattcta cctttctctg gttaaagaaa ctccctgaat ccaccggtta
    10921 tgtcttgacc ggctataagc ctaatgggtc agtcccttat gttacgtcct gtagaaaccc
    10981 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact
    11041 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc
    11101 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct
    11161 ggtatcagcg cgaagtcttt ataccgaaag gtaagtctta ctctctcttt tttggtctgt
    11221 atttttaatt ttttgaagta tactatttgt actgacgcta ataatctttt ttcaggttgg
    11281 gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc
    11341 aataatcagg aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg
    11401 ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac
    11461 tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct
    11521 tacttccatg atttctttaa ctatgccgga atccatcgca gcgtaatgct ctacaccacg
    11581 ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac
    11641 gcgtctgttg actggcaggt acttcatgct tcaacgtgta acttaagaga tactgtgtga
    11701 aattttatat ttccatacat ttgcttgacc tttgcttttt gtcaattttt ttccccttac
    11761 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg
    11821 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg
    11881 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct
    11941 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc
    12001 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag
    12061 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact
    12121 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg
    12181 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt
    12241 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc
    12301 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg
    12361 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt
    12421 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca
    12481 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc
    12541 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac
    12601 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat
    12661 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
    12721 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg
    12781 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg
    12841 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc
    12901 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgag
    12961 cttgtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
    13021 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
    13081 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
    13141 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
    13201 cggtgtcatc tatgttacta gatcgacgct actagaggat gcacatgtga cc
//
