LOCUS       sequence-468635-       10688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA083
FEATURES             Location/Qualifiers
     source          1..10688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      643..652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(649..651,652..654,655..1365)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(649..651,652..654,655..1365)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(694..715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(955..974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1302..1323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1412..1546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1436..1455)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1490..1509
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1689..1711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2271..4060
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4362..6287)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6722..6740)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6808..6912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6913..6981,6982..7773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7131..7150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7944..8532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8433..8452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8963..9108
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9151..9945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9205..9224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9815..9834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9987..10035
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgctaccgg tcgccaccat ggtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
     1381 cgagctcaag cttcgaatag cggccgctaa ggatccagac atgataagat acattgatga
     1441 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1501 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1561 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     1621 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
     1681 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     1741 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     1801 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     1861 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     1921 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     1981 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     2041 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     2101 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     2161 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     2221 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     2281 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     2341 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     2401 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     2461 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     2521 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     2581 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     2641 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     2701 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     2761 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     2821 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     2881 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     2941 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     3001 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     3061 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     3121 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     3181 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     3241 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     3301 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     3361 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     3421 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3481 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     3541 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     3601 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     3661 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     3721 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     3781 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     3841 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     3901 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
     3961 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
     4021 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
     4081 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
     4141 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
     4201 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
     4261 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
     4321 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
     4381 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
     4441 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
     4501 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
     4561 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
     4621 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
     4681 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
     4741 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
     4801 ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc
     4861 gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct
     4921 ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc
     4981 tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat
     5041 tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg
     5101 cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca
     5161 cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct
     5221 ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
     5281 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc
     5341 cctcctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     5401 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     5461 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     5521 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     5581 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     5641 cctgctcctg cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     5701 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct
     5761 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     5821 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     5881 gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
     5941 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     6001 cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga
     6061 cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg
     6121 gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca
     6181 ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc
     6241 tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa
     6301 agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct
     6361 gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag
     6421 gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc
     6481 ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa
     6541 ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc
     6601 ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc
     6661 ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
     6721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7141 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7441 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8581 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     8641 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     8701 gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc
     8761 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
     8821 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
     8881 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata
     8941 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa
     9001 ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt
     9061 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag
     9121 cgtgccgcag atcccggggg gcaatgagat atgattgaac aagatggatt gcacgcaggt
     9181 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     9241 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     9301 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     9361 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     9421 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     9481 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     9541 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     9601 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     9661 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9721 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9781 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9841 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9901 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctaaaacac ggaaggagac
     9961 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
    10021 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
    10081 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
    10141 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
    10201 ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg
    10261 ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc
    10321 atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg
    10381 tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca
    10441 agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt
    10501 gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg
    10561 gcaattagcc atattagtca ttggttatat agcataaatc aatattggct attggccatt
    10621 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc
    10681 gccatgtt
//
