LOCUS       sequence-468701-       16004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1RP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 16004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0372-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-02"; volume: "27"; issue: "2"; pages: "168-178"
COMMENT     Sequence Label: pDA077
FEATURES             Location/Qualifiers
     source          1..16004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    6706..6840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6730..6749)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6784..6803
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6983..7005)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7569..9358
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(12038..12056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12124..12228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12229..12297,12298..13089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12447..12466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13260..13848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13749..13768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        14279..14424
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             14467..15261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14521..14540)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15131..15150
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15303..15351
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcaagcttgc tagcggccgc tctagccctg
      661 gaatgtgtat taagactgta aggtgggggg gaggagccaa gatggccgaa taggaacagc
      721 tccggtctac agctcccagc gtgagcgacg cagaagacgg tgatttctgc atttccatct
      781 gaggtaccgg gttcatctca ctagggagtg ccagacagtg ggcgcaggcc agtgtgtgtg
      841 cgcaccgtgc gcgagccgaa gcagggcgag gcattgcctc acctgggaag cgcaaggggt
      901 cagggagttc cctttccgag tcaaagaaag gggtgacgga cgcacctgga aaatcgggtc
      961 actcccaccc gaatattgcg cttttcagac cggcttaaga aacggcgcac cacgagacta
     1021 tatcccgcac ctggctcgga gggtcctacg cccacggaat ctcgctgatt gctagcacag
     1081 cagtctgaga tcaaactgca aggcggcaac gaggctgggg gaggggcgcc cgccattgcc
     1141 caggcttgct taggtaaaca aagcagcagg gaagctcgaa ctgggtggag cccaccacag
     1201 ctcaaggagg cctgcctgcc tctgtaggct ccacctctgg gggcagggca cagacaaaca
     1261 aaaagacagc agtaacctct gcagacttaa gtgtccctgt ctgacagctt tgaagagagc
     1321 agtggttctc ccagcacgca gctggagatc tgagaacggg cagactgcct cctcaagtgg
     1381 gtccctgacc cctgaccccc gagcagccta actgggaggc accccccagc aggggcacac
     1441 tgacacctca cacggcaggg tattccaaca gacctgcagc tgagggtcct gtctgttaga
     1501 aggaaaacta acaaccagaa aggacatcta caccgaaaac ccatctgtac atcaccatca
     1561 tcaaagacca aaagtagata aaaccacaaa gatggggaaa aaacagaaca gaaaaactgg
     1621 aaactctaaa acgcagagcg cctctcctcc tccaaaggaa cgcagttcct caccagcaac
     1681 agaacaaagc tggatggaga atgattttga tgagctgaga gaagaaggct tcagacgatc
     1741 aaattactct gagctacggg aggacattca aaccaaaggc aaagaagttg aaaactttga
     1801 aaaaaattta gaagaatgta taactagaat aaccaataca gagaagtgct taaaggagct
     1861 gatggagctg aaaaccaagg ctcgagaact acgtgaagaa tgcagaagcc tcaggagccg
     1921 atgcgatcaa ctggaagaaa gggtatcagc aatggaagat gaaatgaatg aaatgaagcg
     1981 agaagggaag tttagagaaa aaagaataaa aagaaatgag caaagcctcc aagaaatatg
     2041 ggactatgtg aaaagaccaa atctacgtct gattggtgta cctgaaagtg atgtggagaa
     2101 tggaaccaag ttggaaaaca ctctgcagga tattatccag gagaacttcc ccaatctagc
     2161 aaggcaggcc aacgttcaga ttcaggaaat acagagaacg ccacaaagat actcctcgag
     2221 aagagcaact ccaagacaca taattgtcag attcaccaaa gttgaaatga aggaaaaaat
     2281 gttaagggca gccagagaga aaggtcgggt taccctcaaa ggaaagccca tcagactaac
     2341 agcggatctc tcggcagaaa ccctacaagc cagaagagag tgggggccaa tattcaacat
     2401 tcttaaagaa aagaattttc aacccagaat ttcatatcca gccaaactaa gcttcataag
     2461 tgaaggagaa ataaaatact ttatagacaa gcaaatgttg agagattttg tcaccaccag
     2521 gcctgcccta aaagagctcc tgaaggaagc gctaaacatg gaaaggaaca accggtacca
     2581 gccgctgcaa aatcatgcca aaatgtaaag accatcaaga ctaggaagaa actgcaccaa
     2641 ctaatgagca aaatcaccag ctaacatcat aatgacagga tcaacttcac acataacaat
     2701 attaacttta aatataaatg gactaaattc tgcaattaaa agacacagac tggcaagttg
     2761 gataaagagt caagacccat cagtgtgctg tattcaggaa acccatctca cgtgcagaga
     2821 cacacatagg ctcaaaataa aaggatggag gaagatctac caagccaatg gaaaacaaaa
     2881 aaaggcaggg gttgcaatcc tagtctctga taaaacagac tttaaaccaa caaagatcaa
     2941 aagagacaaa gaaggccatt acataatggt aaagggatca attcaacaag aggagctaac
     3001 tatcctaaat atttatgcac ccaatacagg agcacccaga ttcataaagc aagtcctcag
     3061 tgacctacaa agagacttag actcccacac attaataatg ggagacttta acaccccact
     3121 gtcaacatta gacagatcaa cgagacagaa agtcaacaag gatacccagg aattgaactc
     3181 agctctgcac caagcagacc taatagacat ctacagaact ctccacccca aatcaacaga
     3241 atatacgttt ttttcagcac cacaccacac ctattccaaa attgaccaca tagttggaag
     3301 caaagctctc ctcagcaaat gtaaaagaac agaaattata acaaactatc tctcagacca
     3361 cagtgcaatc aaactagagc tcaggattaa gaatctcact caaagccgct caactacatg
     3421 gaaactgaac aacctgctcc tgaatgacta ctgggtacat aacgaaatga aggcagaaat
     3481 aaagatgttc tttgaaacca acgagaacaa agacaccaca taccagaatc tctgggacgc
     3541 attcaaagca gtatgtagag ggaaatttat agcactaaat gcctacaaga gaaagcagga
     3601 aagatccaaa attgacaccc taacatcaca attaaaagaa ctagaaaagc aagagcaaac
     3661 acattcaaaa gctagcagaa ggcaagaaat aactaaaatc agagcagaac tgaaggaaat
     3721 agagacacaa aaaacccttc aaaaaatcaa tgaatccagg agctggtttt ttgaaaggat
     3781 caacaaaatt gatagaccgc tagcaagact aataaagaaa aaaagagaga agaatcaaat
     3841 agacacaata aaaaatgata aaggggatat caccaccgat cccacagaaa tacaaactac
     3901 catcagagaa tactacaaac acctctacgc aaataaacta gaaaatctag aagaaatgga
     3961 tacattcctc gacacataca ctctcccaag actaaaccag gaagaagttg aatctctgaa
     4021 tcgaccaata acaggctctg aaattgtggc aataatcaat agtttaccaa ccaaaaagag
     4081 tccaggacca gatggattca cagccgaatt ctaccagagg tacaaggagg aactggtacc
     4141 attccttctg aaactattcc aatcaataga aaaagaggga atcctcccta actcatttta
     4201 tgaggccagc atcattctga taccaaagcc gggcagagac acaaccaaaa aagagaattt
     4261 tagaccaata tccttgatga acattgatgc aaaaatcctc aataaaatac tggcaaaccg
     4321 aatccagcag cacatcaaaa agcttatcca ccatgatcaa gtgggcttca tccctgggat
     4381 gcaaggctgg ttcaatatac gcaaatcaat aaatgtaatc cagcatataa acagagccaa
     4441 agacaaaaac cacatgatta tctcaataga tgcagaaaaa gcctttgaca aaattcaaca
     4501 acccttcatg ctaaaaactc tcaataaatt aggtattgat gggacgtatt tcaaaataat
     4561 aagagctatc tatgacaaac ccacagccaa tatcatactg aatgggcaaa aactggaagc
     4621 attccctttg aaaaccggca caagacaggg atgccctctc tcaccgctcc tattcaacat
     4681 agtgttggaa gttctggcca gggcaatcag gcaggagaag gaaataaagg gtattcaatt
     4741 aggaaaagag gaagtcaaat tgtccctgtt tgcagacgac atgattgttt atctagaaaa
     4801 ccccatcgtc tcagcccaaa atctccttaa gctgataagc aacttcagca aagtctcagg
     4861 atacaaaatc aatgtacaaa aatcacaagc attcttatac accaacaaca gacaaacaga
     4921 gagccaaatc atgggtgaac tcccattcac aattgcttca aagagaataa aatacctagg
     4981 aatccaactt acaagggatg tgaaggacct cttcaaggag aactacaaac cactgctcaa
     5041 ggaaataaaa gaggagacaa acaaatggaa gaacattcca tgctcatggg taggaagaat
     5101 caatatcgtg aaaatggcca tactgcccaa ggtaatttac agattcaatg ccatccccat
     5161 caagctacca atgactttct tcacagaatt ggaaaaaact actttaaagt tcatatggaa
     5221 ccaaaaaaga gcccgcattg ccaagtcaat cctaagccaa aagaacaaag ctggaggcat
     5281 cacactacct gacttcaaac tatactacaa ggctacagta accaaaacag catggtactg
     5341 gtaccaaaac agagatatag atcaatggaa cagaacagag ccctcagaaa taatgccgca
     5401 tatctacaac tatctgatct ttgacaaacc tgagaaaaac aagcaatggg gaaaggattc
     5461 cctatttaat aaatggtgct gggaaaactg gctagccata tgtagaaagc tgaaactgga
     5521 tcccttcctt acaccttata caaaaatcaa ttcaagatgg attaaagatt taaacgttaa
     5581 acctaaaacc ataaaaaccc tagaagaaaa cctaggcatt accattcagg acataggcgt
     5641 gggcaaggac ttcatgtcca aaacaccaaa agcaatggca acaaaagaca aaattgacaa
     5701 atgggatcta attaaactaa agagcttctg cacagcaaaa gaaactacca tcagagtgaa
     5761 caggcaacct acaacatggg agaaaatttt tgcaacctac tcatctgaca aagggctaat
     5821 atccagaatc tacaatgaac tcaaacaaat ttacaagaaa aaaacaaaca accccatcaa
     5881 aaagtgggcg aaggacatga acagacactt ctcaaaagaa gacatttatg cagccaaaaa
     5941 acacatgaag aaatgctcat catcactggc catcagagaa atgcaaatca aaaccactat
     6001 gagatatcat ctcacaccag ttagaatggc aatcattaaa aagtcaggaa acaacaggtg
     6061 ctggagagga tgcggagaaa taggaacact tttacactgt tggtgggact gtaaactagt
     6121 tcaaccattg tggaagtcag tgtggcgatt cctcagggat ctagaactag aaataccatt
     6181 tgacccagcc atcccattac tgggtatata cccaaatgag tataaatcat gctgctataa
     6241 agacacatgc acacgtatgt ttattgcggc actattcaca atagcaaaga cttggaacca
     6301 acccaaatgt ccaacaatga tagactggat taagaaaatg tggcacatat acaccatgga
     6361 atactatgca gccataaaaa atgatgagtt catatccttt gtagggacat ggatgaaatt
     6421 ggaaaccatc attctcagta aactatcgca agaacaaaaa accaaacacc gcatattctc
     6481 actcataggt gggaattgaa caatgagatc acatggacac aggaagggga atatcacact
     6541 ctggggactg tggtggggtc gggggagggg ggagggatag cattgggaga tatacctaat
     6601 gctagatgac acattagtgg gtgcagcgca ccagcatggc acatgtatac atatgtaact
     6661 aacctgcaca atgtgcacat gtaccctaaa acttagagta taatagatcc agacatgata
     6721 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6781 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6841 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     6901 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     6961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7081 ggtgtcgggg cgcagccatg aggtcgatcg actctagagg atcgatcccc gccccggacg
     7141 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     7201 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     7261 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     7321 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     7381 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     7441 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     7501 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     7561 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     7621 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     7681 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     7741 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     7801 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     7861 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     7921 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     7981 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     8041 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     8101 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     8161 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     8221 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     8281 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     8341 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     8401 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     8461 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     8521 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     8581 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     8641 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     8701 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     8761 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     8821 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     8881 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     8941 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     9001 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     9061 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9121 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9181 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     9241 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
     9301 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
     9361 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
     9421 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
     9481 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
     9541 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
     9601 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
     9661 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
     9721 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
     9781 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
     9841 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
     9901 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
     9961 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    10021 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    10081 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    10141 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    10201 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    10261 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    10321 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    10381 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    10441 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    10501 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    10561 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    10621 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    10681 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
    10741 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
    10801 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
    10861 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
    10921 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct
    10981 cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
    11041 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
    11101 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
    11161 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
    11221 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
    11281 cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt
    11341 ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca
    11401 tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt
    11461 cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg
    11521 ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct
    11581 gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta
    11641 gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc
    11701 ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga
    11761 accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc
    11821 aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga
    11881 cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga
    11941 tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga
    12001 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
    12061 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    12121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    12181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    12241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    12301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    12361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    12421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
    12481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    12541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    12601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    12661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    12721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
    12781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    12841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    12901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    12961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    13021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    13081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    13141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    13201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    13261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    13321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    13381 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    13441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    13501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    13561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    13621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    13681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    13741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    13801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    13861 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
    13921 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
    13981 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
    14041 agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
    14101 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
    14161 ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg
    14221 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
    14281 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
    14341 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
    14401 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa
    14461 tgagatatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
    14521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
    14581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
    14641 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
    14701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
    14761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
    14821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
    14881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
    14941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
    15001 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    15061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    15121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    15181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    15241 cttcttgacg agttcttcta aaacacggaa ggagacaata ccggaaggaa cccgcgctat
    15301 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
    15361 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    15421 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    15481 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
    15541 ggggtacccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
    15601 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
    15661 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
    15721 cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa ttctcatgtt
    15781 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg
    15841 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg
    15901 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
    15961 tatgtacatt tatattggct catgtccaat atgaccgcca tgtt
//
