LOCUS       sequence-468704-        3931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v16-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO231
FEATURES             Location/Qualifiers
     source          1..3931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1664..1851
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1719..1743)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(2168..2959,2960..3028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2791..2810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3029..3133)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3236..3824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3725..3744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagat ggtagagcca caaacagccg gtacaagcaa cgatctccag
       61 gaccatctga atcatgcgcg gatgacacga actcacgacg gcgatcacag acattaaccc
      121 acagtacaga cactgcgaca acgtggcaat tcgtcgcaat accgtctcac tgaactggcc
      181 gataattgca gacgaacgaa gaggatgtcc aatatttttt ttaaggaata aggatacttc
      241 aagactagat tcccccctgc attcccatca gaaccgtaaa ccttggcgct ttccttggga
      301 agtattcaag aagtgccttg tccggtttct gtggctcaca aaccagcgcg cccgatatgg
      361 ctttcttttc acttatgaat gtaccagtac gggacaatta gaacgctcct gtaacaatct
      421 ctttgcaaat gtggggttac attctaacca tgtcacactg ctgacgaaat tcaaagtaaa
      481 aaaaaatggg accacgtctt gagaacgata gattttcttt attttacatt gaacagtcgt
      541 tgtctcagcg cgctttatgt tttcattcat acttcatatt ataaaataac aaaagaagaa
      601 tttcatattc acgcccaaga aatcaggctg ctttccaaat gcaattgaca cttcattagc
      661 catcacacaa aactctttct tgctggagct tcttttaaaa aagacctcag tacaccaaac
      721 acgttacccg acctcgttat tttacgacaa ctatgataaa attctgaaga aaaaataaaa
      781 aaattttcat acttcttgct tttatttaaa ccattgaatg atttcttttg aacaaaacta
      841 cctgtttcac caaaggaaat agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag
      901 atctatgagt cgtcttgata agtccaagat cataaatagc gcactggaat tgctgaacgg
      961 cgttggtatt gagggtctta ccacgagaaa gctggcacag aagttggggg tcgaacaacc
     1021 gaccttgtac tggcatgtca agaataagcg tgcgctatta gatgccttac ctatcgagat
     1081 gctagacaga catcatactc actcatgtcc attggagggg gagtcttggc aagactttct
     1141 tagaaacaat gcaaagtcat ataggtgcgc gctactatca catagagatg gggccaaagt
     1201 ccatttgggc accaggccga ccgaaaagca gtacgagact ttagagaacc agctggcatt
     1261 cctgtgtcaa caaggcttca gtctagagaa tgctctgtat gctttaagcg cggtcggcca
     1321 cttcacgcta ggttgtgtat tagaggaaca agaacaccaa gtggcaaaag aggaaagaga
     1381 aactccaacg accgacagca tgccaccact gttgaaacaa gcaatcgaac tgtttgatcg
     1441 tcaaggggct gaaccagcat tcctattcgg tttggaatta ataatctgcg gtctggagaa
     1501 acaattgaag tgcgaaagcg gcgggccgac agatgcctta gatgactttg acctagatat
     1561 gttaccagcc gacgcccttg acgactttga tctggacatg ctgccggctg acgccttgga
     1621 tgacttcgac ttggatatgt tgccaggggg atcctaactc gaggcgaatt tcttatgatt
     1681 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     1741 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     1801 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag
     1861 caaatgcctg caaatcgctc cccatttcgc tgccaatgag acgacggggt catcacggct
     1921 catcatgcgc caaacaaatg tgtgcaatac acgctcggat gactgcatga tgaccgcact
     1981 gactggggac agcagatcca cctaagcctg tgagagaagc agacacccga cagatcaagg
     2041 cagttaacta gtgcactgca gtacagcggc cgcgattatc aaaaaggatc ttcacctaga
     2101 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2281 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag
     2341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3121 taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa atcccttaac
     3181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3901 cccctgattc tgtggataac cgtgcggccg c
//
