LOCUS       sequence-468705-        3892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA3-tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO230
FEATURES             Location/Qualifiers
     source          1..3892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1625..1812
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1680..1704)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(2129..2920,2921..2989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2752..2771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2990..3094)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3197..3785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3686..3705
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagat ggtagagcca caaacagccg gtacaagcaa cgatctccag
       61 gaccatctga atcatgcgcg gatgacacga actcacgacg gcgatcacag acattaaccc
      121 acagtacaga cactgcgaca acgtggcaat tcgtcgcaat accgtctcac tgaactggcc
      181 gataattgca gacgaacgaa gaggatgtcc aatatttttt ttaaggaata aggatacttc
      241 aagactagat tcccccctgc attcccatca gaaccgtaaa ccttggcgct ttccttggga
      301 agtattcaag aagtgccttg tccggtttct gtggctcaca aaccagcgcg cccgatatgg
      361 ctttcttttc acttatgaat gtaccagtac gggacaatta gaacgctcct gtaacaatct
      421 ctttgcaaat gtggggttac attctaacca tgtcacactg ctgacgaaat tcaaagtaaa
      481 aaaaaatggg accacgtctt gagaacgata gattttcttt attttacatt gaacagtcgt
      541 tgtctcagcg cgctttatgt tttcattcat acttcatatt ataaaataac aaaagaagaa
      601 tttcatattc acgcccaaga aatcaggctg ctttccaaat gcaattgaca cttcattagc
      661 catcacacaa aactctttct tgctggagct tcttttaaaa aagacctcag tacaccaaac
      721 acgttacccg acctcgttat tttacgacaa ctatgataaa attctgaaga aaaaataaaa
      781 aaattttcat acttcttgct tttatttaaa ccattgaatg atttcttttg aacaaaacta
      841 cctgtttcac caaaggaaat agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag
      901 atctatgagt cgtttagata aatccaaggt tataaacggc gcactagaac tgcttaatgg
      961 cgttggtatc gaaggtctga ctacgaggaa gttagcacag aaacttgggg tcgagcagcc
     1021 cacgctgtat tggcacgtaa agaacaagag agcgctatta gatgccctac caatagagat
     1081 gttagataga caccatactc atttttgccc gcttgagggg gaaagctggc aagacttttt
     1141 aagaaataat gcaaagagtt acaggtgcgc tcttctttca catagggatg gggcaaaggt
     1201 acacttaggt acgaggccaa ccgagaaaca gtatgaaaca ctagaaaacc aacttgcttt
     1261 tttatgccaa cagggtttca gtcttgagaa tgcactttat gcccttagcg cagtcgggca
     1321 ctttacgttg ggttgcgtcc tagaggagca ggaacatcaa gtcgcaaaag aggaacgtga
     1381 gacaccgact actgatagta tgcccccgtt attacgtcag gcaatagaac tatttgaccg
     1441 tcaaggggcg gaaccggctt ttctttttgg cctggagcta attatttgtg ggctggagaa
     1501 gcaactgaag tgcgaaagtg gtgggccaac tgacgcgcta gatgatttcg atcttgatat
     1561 gttacctgca gatgcactag atgactttga tttagacatg ttgcctggag gatcctaact
     1621 cgaggcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     1681 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     1741 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     1801 cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttcg ctgccaatga
     1861 gacgacgggg tcatcacggc tcatcatgcg ccaaacaaat gtgtgcaata cacgctcgga
     1921 tgactgcatg atgaccgcac tgactgggga cagcagatcc acctaagcct gtgagagaag
     1981 cagacacccg acagatcaag gcagttaact agtgcactgc agtacagcgg ccgcgattat
     2041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg gacccacgct
     2281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3121 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3181 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3241 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3301 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3361 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3421 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3481 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3541 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3601 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3661 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3781 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3841 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gc
//
