LOCUS       sequence-468714-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-YTK; type3A; Auxin systems; AmtR-NES .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   O'Laughlin R, Tran Q, Lezia A, Ngamkanjanarat W, Emmanuele P, Hao N,
            Hasty J
  TITLE     A Standardized Set of MoClo-Compatible Inducible Promoter Systems 
            for Tunable Gene Expression in Yeast.
  JOURNAL   ACS Synth Biol. 2024 Jan 19;13(1):85-102. doi: 
            10.1021/acssynbio.3c00184. Epub 2023 Dec 11.
  PUBMED    38079574
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00184"; journalName: "ACS Synth Biol"; date: 
            "2024-01-19- 19"; volume: "13"; issue: "1"; pages: "85-102"
COMMENT     Sequence Label: pRO271
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             145..171
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     terminator      complement(191..225)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(296..955)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     870..889
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaagtgcag atagtcttcc agaaaccgca atcatgttgg ctgacgccag cttggccgtc
       61 ctgggagcac ccctaccggc cgatagggtt gaaaagacgc ttgaattgat taaacaggca
      121 gacgcaaagg caagcggaag cggactgcct ccgttagaaa gactaacgct gggttcttga
      181 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      241 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      301 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      361 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      421 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      481 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      541 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      601 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      661 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      721 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      781 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      841 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      901 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      961 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1021 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg ctggtgctgt gggaaggcca
     1861 aggaggtccg cccccaggag agcggggaaa aatcctcgtg aagagatact ggacgcatct
     1921 gctgagcttt ttacgcgtca aggctttgct actacttcaa ctcaccagat agcagatgcg
     1981 gtcggcatta gacaggcttc tctatattat cattttccgt caaaaacgga gattttcctt
     2041 acactactta agtctacagt tgagccttca actgtcttgg cagaggatct atccaccctt
     2101 gacgccggtc ccgagatgag attgtgggct atcgtagcga gtgaggtgcg tttattgcta
     2161 tctacaaaat ggaatgtagg aaggctatac caattgccta tcgtgggctc agaggagttc
     2221 gctgaatacc attcccagag ggaggcgctt acaaatgtct ttcgtgatct tgctacagaa
     2281 attgtaggag atgaccccag agcggagctg cccttccaca taactatgag cgtgatagag
     2341 atgcgtagga acgatggaaa gattccaagt cca
//
