LOCUS       sequence-468728-        5653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FtsZ without a peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FtsZ
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(80..94,95..100,101..117,118..124)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    132..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             167..178
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1418..1512
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1582..1604)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        2069..2171
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2172..2831
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2238..2257)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3382..3970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3871..3890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4124..4141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4214..4291
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4292..5374
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4311..4330)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5423..5428,5429..5446,5447..5453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5461..5477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5485..5501
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5485..5501
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5517..5534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5517..5533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5526..5548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactactagt atgtttgaac caatggaact taccaatgac gcggtgatta aagtcatcgg
      241 cgtcggcggc ggcggcggta atgctgttga acacatggtg cgcgagcgca ttgaaggtgt
      301 tgaattcttc gcggtaaata ccgatgcaca agcgctgcgt aaaacagcgg ttggacagac
      361 gattcaaatc ggtagcggta tcaccaaagg actgggcgct ggcgctaatc cagaagttgg
      421 ccgcaatgcg gctgatgagg atcgcgatgc attgcgtgcg gcgctggaag gtgcagacat
      481 ggtctttatt gctgcgggta tgggtggtgg taccggtaca ggtgcagcac cagtcgtcgc
      541 tgaagtggca aaagatttgg gtatcctgac cgttgctgtc gtcactaagc ctttcaactt
      601 tgaaggcaag aagcgtatgg cattcgcgga gcaggggatc actgaactgt ccaagcatgt
      661 ggactctctg atcactatcc cgaacgacaa actgctgaaa gttctgggcc gcggtatctc
      721 cctgctggat gcgtttggcg cagcgaacga tgtactgaaa ggcgctgtgc aaggtatcgc
      781 tgaactgatt actcgtccgg gtttgatgaa cgtggacttt gcagacgtac gcaccgtaat
      841 gtctgagatg ggctacgcaa tgatgggttc tggcgtggcg agcggtgaag accgtgcgga
      901 agaagctgct gaaatggcta tctcttctcc gctgctggaa gatatcgacc tgtctggcgc
      961 gcgcggcgtg ctggttaaca tcacggcggg cttcgatctg cgtctggatg agttcgaaac
     1021 ggtaggtaac accatccgtg catttgcttc cgacaacgcg actgtggtta tcggtacttc
     1081 tcttgacccg gatatgaatg acgagctgcg cgtaaccgtt gttgcgacag gtatcggcat
     1141 ggacaaacgt cctgaaatca ctctggtgac caataagcag gttcagcagc cagtgatgga
     1201 tcgctaccag cagcatggga tggctccgct gacccaggag cagaagccgg ttgctaaagt
     1261 cgtgaatgac aatgcgccgc aaactgcgaa agagccggat tatctggata tcccagcatt
     1321 cctgcgtaag caagctgatg gcggcagcgg cagcggcagc agctgataag cggccgctaa
     1381 gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta
     1441 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     1501 attggtgaga atccaagcta ggggaattca tcgtgactga ctgacgatct gcctcgcgcg
     1561 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1621 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1681 gtgtcggggc gcagccatga cccggtcacg tagcgatagc ggagtgtata attcttgaag
     1741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1801 ttaggatccg ggatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga
     1861 acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac
     1921 actaaatcag taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga
     1981 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     2041 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     2101 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     2161 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     2221 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     2281 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     2341 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     2401 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     2461 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     2521 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2581 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     2641 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2701 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2761 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2821 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     2881 gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
     2941 cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
     3001 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
     3061 tctccccgtg gagataataa ttgacgttat gatcatttat tctgcctccc agagcctgat
     3121 aaaaacggtt accggatcga gtcaggcaac tatggatgaa cgaaatagac agatcgctta
     3181 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3241 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3301 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3661 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcacaca
     3721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4141 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4201 ccgcataaat tccgacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
     4261 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     4321 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     4381 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     4441 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     4501 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     4561 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     4621 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     4681 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     4741 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     4801 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     4861 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     4921 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     4981 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     5041 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     5101 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     5161 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     5221 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     5281 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaactaat gtaagttagc
     5401 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5461 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg
     5521 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5581 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5641 tcccaacagt tgc
//
