LOCUS       sequence-468741-        6483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To Express a Rat Nurr1-DsRed fusion protein from a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6483)
  TITLE     Ploski Lab Plasmids 2025
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDsRed1-CMV-Rat Nurr1-DsRed1-pA
FEATURES             Location/Qualifiers
     source          1..6483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     334..353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        608..911
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        912..1115
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1066..1086
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3011..3020
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3017..3019,3020..3022,3023..3697)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(3200..3220)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3418..3437
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3612..3635
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3818..3939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3855..3874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3909..3928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3946..4401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4083..4104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4295..4314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4428..4532
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4534..4891
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4534..4725
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4557..4577)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4742..4877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4926..5720
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4926..5720
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4980..4999)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5590..5609
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5908..5927)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5952..5999
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(6328..6483,1..433)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
       61 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      541 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      601 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      661 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      721 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      781 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      841 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      901 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      961 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     1021 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1081 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
     1141 agcgctaccg gactcagatc tcgagatggc ttgtgttcag gcgcagtatg ggtcctcgcc
     1201 tcaaggagcc agccccgctt ctcagagcta cagttaccac tcttcgggag aatacagctc
     1261 cgatttctta actccagagt ttgtcaagtt tagcatggac ctcaccaaca ctgaaattac
     1321 tgccaccact tctctcccca gcttcagtac ctttatggac aactacagca caggctacga
     1381 cgtcaagcca ccttgcttgt accaaatgcc cctgtccgga cagcagtcct ccattaaggt
     1441 agaagacatt cagatgcaca actaccagca acacagccac ctgccccctc agtccgagga
     1501 gatgatgcca cacagcgggt cggtttacta caagccctct tcgcccccga cacccagcac
     1561 cccgggcttc caggtgcagc atagcccgat gtgggacgat ccgggctccc ttcacaactt
     1621 ccaccagaac tacgtggcca ctacgcatat gatcgagcag aggaagacac ctgtctcccg
     1681 cctttcactc ttctccttta agcagtcgcc cccgggcact cctgtgtcta gctgccagat
     1741 gcgctttgac gggcctctgc acgtccccat gaacccggag cccgcgggca gccaccacgt
     1801 agtggatggg cagaccttcg ccgtgcccaa tcccattcgc aagccggcat ccatgggctt
     1861 cccgggcctg cagatcggcc acgcgtcgca gttgcttgac acgcaggtgc cctcgccgcc
     1921 gtcccggggc tctccctcca atgagggtct gtgcgctgtt tgcggtgaca acgcggcctg
     1981 tcagcattac ggtgttcgca cttgtgaggg ctgcaaaggt ttctttaagc gcacggtgca
     2041 aaaaaacgcg aaatatgtgt gtttagcaaa taaaaattgc ccagtggata agcgccgccg
     2101 aaatcgttgt cagtactgtc ggtttcagaa gtgcctggct gttgggatgg ttaaagaagt
     2161 ggttcgcacg gacagtttaa aaggccggag aggtcgtcta ccctcaaaac cgaagagccc
     2221 acaggatccc tctcccccct cacctccggt gagtctgatc agtgccctcg tcagagccca
     2281 cgtcgactcc aatccggcaa tgaccagcct ggactattcc aggttccagg caaaccctga
     2341 ctatcagatg agtggagatg atactcaaca tatccagcag ttctacgatc tcctgactgg
     2401 ctctatggag atcatcagag ggtgggcaga gaagattcct ggctttgctg acctgcccaa
     2461 agccgatcag gacctgcttt ttgaatcagc tttcttagaa ttatttgttc tacgcttagc
     2521 atacaggtcc aacccagtgg agggtaaact catcttttgc aatggggtgg tcttgcacag
     2581 gttgcaatgc gtgcgtggct ttggggaatg gattgattcc attgttgaat tctcctccaa
     2641 cttgcagaat atgaacatcg acatttctgc cttctcctgc attgctgccc tggctatggt
     2701 cacagagaga cacgggctca aggaacccaa gagagtggaa gagctacaaa acaaaattgt
     2761 aaattgtctt aaagaccatg tgactttcaa taatggggga ttgaaccgac ccaactacct
     2821 gtccaaactg ttggggaagc tcccagaact tcgcaccctt tgcacacagg ggctccagcg
     2881 cattttctac ctgaaattgg aagacttggt accaccacca gcaataattg acaaactttt
     2941 cctggacacc ttacctttca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     3001 tccaccggtc gccaccatgg tgcgctcctc caagaacgtc atcaaggagt tcatgcgctt
     3061 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3121 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct
     3181 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
     3241 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
     3301 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3361 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
     3421 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     3481 cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct
     3541 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
     3601 cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
     3661 cgagcgcacc gagggccgcc accacctgtt cctgtagcgg ccgcgactct agatcataat
     3721 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3781 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     3961 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4021 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4081 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4141 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     4201 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     4261 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     4321 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4381 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4441 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4501 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     4561 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4621 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4681 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4741 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4801 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4861 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     4921 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4981 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5041 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5101 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5161 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5221 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5281 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5341 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5401 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5461 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5521 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5581 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5641 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5701 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5761 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5821 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5881 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     5941 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6001 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6061 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     6121 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     6181 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6241 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6481 agt
//
