LOCUS       sequence-46885-e        5087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN
  TITLE     Metabolic engineering of cofactor F420 production in Mycobacterium 
            smegmatis.
  JOURNAL   PLoS One. 2010 Dec 29;5(12):e15803.
  PUBMED    21209917
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-29"; volume: "5(12)"; pages: "e15803"
COMMENT     Sequence Label: pYUBDuet
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        291..309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    310..334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             443..487
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(525..543)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      539..586
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      623..1051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(710..729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     920..941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(3167..3755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3247..3266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggcagcagcc atcaccatca tcaccacagc
      181 caggatccga attcgagctc ggcgcgcctg caggtcgaca agcttgcggc cgcataatgc
      241 ttaagtcgaa cagaaagtaa tcgtattgta cacggccgca taatcgaaat taatacgact
      301 cactataggg gaattgtgag cggataacaa ttccccatct tagtatatta gttaagtata
      361 agaaggagat atacatatgg cagatctcaa ttggatatcg gccggccacg cgatcgctga
      421 cgtcggtacc ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
      481 ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact
      541 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      601 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      781 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      841 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      901 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      961 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1021 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tatctagatt taaagatctg
     1081 gtaccgcggc cgcgccacgg atgccaccac aagcactaca acggagttcg ccacgtacat
     1141 caccacaacc accgattctg gcggtgagct ccacgatatt cagcggaaat ggcttggtat
     1201 cgaccaagat tcgtagaacc ccgtctcgtc tggctggtat tcaaaacgga cgcaacgaaa
     1261 cacgcaacga gacaggcatg gcccaaacca gaaaactagc gtctaccagg acttttacct
     1321 gtccgacccg ttgcaacgga accccccacg gaacccccgc gacacccgct ccccaattgc
     1381 gttagaacag cggtggattg tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg
     1441 ccgcacgctc tttcctcgcc cgatagccga gtcgcttaac ggtgtccaga tgcagcccga
     1501 aatgtttggc cgtttgcggc caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt
     1561 cgcggcgtgc agcctgctcg gcgaaccact cgctgcgttc ctgcgccacg agccggacga
     1621 cgtggcgttc ggatagtccg gtgattcgag cgccttcggc ggcggtcacg cgccgctttt
     1681 tgcggacagt cggctgccag ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca
     1741 tagcaatgcc tccatgcatg gctgacgcgg actttgcgcg ccgcgcaact gtgctcgccg
     1801 ccgtgcgcgc tgctgcgccc ttccgcgaga tggccgcatg gcgcgcactg agtgtggcct
     1861 cgtagaccac gatcccgtcc gcccaaatgc gcgacttggt tgtgatccaa cgccaaatgc
     1921 tgttggcgat ggcgcggacc tcgctgtccg gtagcggtcc gggacacacg tcgttgcacg
     1981 gaaattcggc gtttcgcgcg tggcactcgg catagatcgc gcggccgagt ccgtccacgt
     2041 tccgggtcgg caggtagatc cgcatgaggg cgggaagata ggcccacaac ctgacggaat
     2101 cgaacagtgc gcaattccgc cctagcggcg tcggagccgc tttgtacgtg gtctgctgac
     2161 gccagcgcgg cggtggcatg ttcgcgccga gctcggcctc gatgtggctg agtgtgtaga
     2221 gatctgagtg gagccattcc gtttcccagg cgatgtggcc ggggtttttg gtcatgaggc
     2281 ctgagtaact gcggtcgcca tcgacggcgc gccgaaggcc ttcggcgcac gccgccatgt
     2341 atgcgagcgg cttacgccgc gcgtattcgg tgcgtggaac aggggcgttg agtgcccaca
     2401 ctgcgtgtgc gtggccgttg gcgcgattgc ccacgatcgc gttgggcagc ggatgggacc
     2461 cccgggcgct gagcgctcgg agcgctgcgt ctggatggtc tacgtccacg accagcaggt
     2521 ttgccagcgc tgttgggttc gcctcgatgt accggcggcc tagggccgac gcgcggcttt
     2581 ggcggtagat cccctcgagc agatcgtcgc ttgccaggga ccagtacggc agccagagct
     2641 gctcaaattc gtcggcgacg tggctcacgc ttgataataa accacgatta atcaccggtg
     2701 tatggtccga cacgagctcc aagtcagata tttcgctgag gggccacccc acaactgcac
     2761 actcccccgc tctcccgtcg agccctgatg atgaaacacc agcgacagcc gagcaccccc
     2821 aaccacctgt accaaccagg aggaacacat gcgtcgtttc gaggacgttt ccgggccgct
     2881 aagagccgct gtggcggccg tacacgccgc cttagacccg ttagaccccc tgccgcctga
     2941 atgcgcgggt acgagccaca cagcacccga acttacggag ctggtgggct cacgccgtgc
     3001 ggccgcacgc gttgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3361 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3601 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3781 ggtctgacgc tcagtggaac tagagcatga gattatcaaa aaggatcttc acctagatcc
     3841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3961 ccatagttgc ctgactcccc gacgtggccg accagcccgt catcgtcaac gcctgaccgc
     4021 ggtgcggaca ggccgtgtcg cgaccggccg tgcggaatta agccggcccg taccctgtga
     4081 atagaggtcc gctgtgacac aagaatccct gttacttctc gaccgtattg attcggatga
     4141 ttcctacgcg agcctgcgga acgaccagga attctgggag ccgctggccc gccgagccct
     4201 ggaggagctc gggctgccgg tgccgccggt gctgcgggtg cccggcgaga gcaccaaccc
     4261 cgtactggtc ggcgagcccg acccggtgat caagctgttc ggcgagcact ggtgcggtcc
     4321 ggagagcctc gcgtcggagt cggaggcgta cgcggtcctg gcggacgccc cggtgccggt
     4381 gccccgcctc ctcggccgcg gcgagctgcg gcccggcacc ggagcctggc cgtggcccta
     4441 cctggtgatg agccggatga ccggcaccac ctggcggtcc gcgatggacg gcacgaccga
     4501 ccggaacgcg ctgctcgccc tggcccgcga actcggccgg gtgctcggcc ggctgcacag
     4561 ggtgccgctg accgggaaca ccgtgctcac cccccattcc gaggtcttcc cggaactgct
     4621 gcgggaacgc cgcgcggcga ccgtcgagga ccaccgcggg tggggctacc tctcgccccg
     4681 gctgctggac cgcctggagg actggctgcc ggacgtggac acgctgctgg ccggccgcga
     4741 accccggttc gtccacggcg acctgcacgg gaccaacatc ttcgtggacc tggccgcgac
     4801 cgaggtcacc gggatcgtcg acttcaccga cgtctatgcg ggagactccc gctacagcct
     4861 ggtgcaactg catctcaacg ccttccgggg cgaccgcgag atcctggccg cgctgctcga
     4921 cggggcgcag tggaagcgga ccgaggactt cgcccgcgaa ctgctcgcct tcaccttcct
     4981 gcacgacttc gaggtgttcg aggagacccc gctggatctc tccggcttca ccgatccgga
     5041 ggaactggcg cagttcctct gggggccgcc ggacaccgcc cccggcg
//
