LOCUS       sequence-46886-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Astrakhan A, Sather BD, Ryu BY, Khim S, Singh S, Humblet-Baron S, 
            Ochs HD, Miao CH, Rawlings DJ
  TITLE     Ubiquitous high-level gene expression in hematopoietic lineages 
            provides effective lentiviral gene therapy of murine Wiskott-Aldrich
            Syndrome.
  JOURNAL   Blood. 2012 Mar 19.
  PUBMED    22431569
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2012
            Mar 19."
COMMENT     Sequence Label: pRRL MND GFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(88..112)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    516..633
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cctctagagt cggtgtcttc tatggaggtc
       61 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
      121 atagagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
      181 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
      241 ctagaaactg ctgagggcgg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
      301 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttctgctgtt ccaactgttc
      361 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattctg
      421 ctgtctctct gttcggatcc ccataagggc gaattctgca gatatccatc acactggcgg
      481 ccatcaagct tctcgaggct agtctcgtga tcgataaaat tttgaatttt tgtaatttgt
      541 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
      601 ctgtaccccc caatcccccc ttttctttta aaagttaacc gataccgtcg agatccgttc
      661 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
      721 ctgtcgg
//
