LOCUS       sequence-468893-       10041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for bacterial expression and purification of DiCas7-11 
            protein (human codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia
            PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador 
            G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, 
            Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA,
            Moreno-Mateos MA
  TITLE     Enhanced RNA-targeting CRISPR-Cas technology in zebrafish.
  JOURNAL   Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9.
  PUBMED    40091120
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-03-16"; volume: "16(1):2591. doi"; pages: " 
            10.1038/s41467-025-57792-9"
COMMENT     Sequence Label: pET28b-DiCas7-11-His
FEATURES             Location/Qualifiers
     source          1..10041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     230..252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(246..437)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1246..2328)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2290..2309
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2329..2406)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2612..2631
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2715..2734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2715..2733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2734..2758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2773..2795
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7620..7637
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7690..7708)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7704..7751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7788..8243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7875..7894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8085..8106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8335..9150)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8335..9150)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9058..9077
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9142..9161
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9272..9860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9761..9780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10014..10031
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
      301 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
      361 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      421 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
      481 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      541 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      601 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      661 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
      721 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
      781 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
      841 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
      901 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      961 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     1021 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     1081 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     1141 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     1201 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     1261 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1321 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1381 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1441 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1501 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     1561 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1621 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1681 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1741 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1801 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1861 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1921 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1981 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     2041 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     2101 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     2161 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     2221 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     2281 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2341 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2401 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2461 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     2521 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     2581 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     2641 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     2701 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     2761 tctagaaata attttgttta actttaagaa ggagatatac catgaccact accatgaaga
     2821 tcagcatcga gttcttggag cccttccgca tgacgaagtg gcaggagtcc acgcgccgca
     2881 ataagaacaa taaagagttt gtgcgtggcc aagcgtttgc ccgttggcac cgcaacaaga
     2941 aagataacac caagggtcgt ccgtacatta ctggtacgct cctgcgctca gcggtgattc
     3001 gctccgcgga gaacctgctg acactgagtg acggtaagat ctccgagaaa acctgctgcc
     3061 cggggaaatt cgacacggaa gataaggacc gtttactcca gctgcgtcaa cgctccacgc
     3121 tgcgttggac agacaagaat ccgtgtcccg acaatgcgga aacgtattgt ccgttctgtg
     3181 agctcctcgg ccgctccgga aatgacggta agaaagcaga aaagaaggac tggcgcttcc
     3241 gcatccattt tggtaacctt tccctgccgg gcaagcccga cttcgacggt ccgaaggcaa
     3301 ttggttcaca gcgcgtatta aatcgtgtgg attttaagtc gggaaaggca catgacttct
     3361 tcaaggctta tgaggtggac catacccgct tccctcgctt tgaaggggag atcaccatcg
     3421 acaataaagt gtccgccgag gcacgtaagt tgctgtgtga cagccttaaa ttcacggatc
     3481 gcctgtgtgg agccctgtgc gtcatccgct tcgacgagta tacaccggcc gcggacagcg
     3541 gtaagcaaac cgagaatgtg caggcagagc ccaacgcaaa cctggcggag aaaactgccg
     3601 agcaaattat ctcgatcctc gatgacaaca agaaaacgga atacacccgc cttttagcgg
     3661 acgccatccg ctcattgcgt cgctcctcaa aacttgttgc aggtttgccc aaggatcacg
     3721 atggtaagga cgatcattac ttgtgggaca ttgggaagaa gaagaaggat gagaattcgg
     3781 ttaccatccg tcagattctt accacgagtg ccgacacgaa ggagctgaag aacgctggta
     3841 agtggcgcga gttttgtgag aagcttggtg aggcgcttta tttgaagagt aaggatatga
     3901 gcggcgggtt gaagattaca cgccgtattc tcggggacgc agagtttcat ggcaagccag
     3961 atcgcttgga aaagtcccgt agtgtctcga tcggaagcgt gttaaaggaa accgtggtgt
     4021 gcggagaact cgtcgcaaag accccgttct tctttggcgc gatcgacgag gatgcaaaac
     4081 aaactgactt acaggtactg cttactccgg acaataagta tcgcctgccg cgcagcgccg
     4141 tccgcggcat ccttcgccgc gatcttcaaa cgtactttga cagtccctgt aatgcggagt
     4201 taggcggtcg tccatgtatg tgcaagacct gtcgtatcat gcgtggcatt acggttatgg
     4261 acgcgcgctc cgagtataac gcgcccccgg agattcgtca ccgtacccgc attaatccgt
     4321 ttacgggaac agttgctgag ggcgccctgt ttaatatgga agtggccccg gaagggatcg
     4381 tattcccatt ccaattacgc tatcgcggct ctgaggacgg gctcccagac gcgctcaaga
     4441 ccgtgctgaa gtggtgggca gaggggcaag cttttatgtc aggggccgcc agtaccggga
     4501 aaggccgttt tcgtatggag aatgccaaat atgaaacctt ggatctgagc gatgagaatc
     4561 aacgcaatga ctacttgaag aattggggct ggcgtgacga gaagggtctt gaggagctta
     4621 agaaacgttt aaattcaggt ctgccggagc ctggcaatta ccgtgacccg aagtggcatg
     4681 aaatcaacgt gtcaatcgag atggcgagtc ccttcatcaa cggcgaccct atccgcgcag
     4741 ctgtagacaa gcgtgggacg gacgtcgtga ccttcgttaa gtacaaggcg gaaggggaag
     4801 aagcaaagcc cgtgtgcgca tataaggcgg agtcgtttcg cggcgtcatt cgttcggctg
     4861 tagcacgcat tcacatggaa gatggagtgc ccctgactga gcttactcac tcagattgcg
     4921 agtgtttgct ctgtcaaatc ttcgggtcgg aatatgaggc tggtaagatt cgtttcgaag
     4981 atttagtatt tgagagtgac ccggagccgg tcaccttcga tcacgttgca atcgatcgct
     5041 tcaccggcgg tgccgctgac aagaagaagt tcgacgacag tcctttgcca gggtcgccgg
     5101 cgcgccctct gatgctgaag ggttccttct ggattcgccg tgacgttctc gaagatgagg
     5161 agtattgcaa agcgctcggg aaagcattag ccgatgtaaa taatggttta tatcctctgg
     5221 gtggaaaatc cgccatcgga tacggacaag ttaagagcct gggtatcaag ggcgacgata
     5281 agcgtatcag ccgtttaatg aatccggcct tcgacgaaac cgacgtggcg gttccagaga
     5341 agcctaaaac cgatgccgag gtccgtatcg aagctgagaa ggtatattat ccccactact
     5401 ttgtggaacc gcacaagaag gtagagcgcg aggagaagcc gtgcggtcat caaaagtttc
     5461 atgagggacg ccttaccggt aagattcgtt gtaaacttat tacgaaaacg cctctcattg
     5521 tgccggacac cagcaatgat gacttctttc gccctgcgga caaagaggcc cgcaaggaga
     5581 aggacgagta tcacaaatcc tatgccttct ttcgcctgca taagcaaatc atgatccccg
     5641 gtagtgaact ccgcgggatg gtgtcctctg tttacgaaac ggttacaaat tcatgtttcc
     5701 gtatctttga cgagactaag cgtcttagtt ggcgcatgga tgccgatcac caaaacgttt
     5761 tacaagattt cttaccgggc cgtgttacgg ccgacggcaa gcacattcaa aagttttccg
     5821 agactgcacg tgtaccgttt tatgacaaaa cgcaaaagca ctttgacatt ctcgacgagc
     5881 aggaaattgc cggtgaaaag ccggtccgca tgtgggtgaa gcgtttcatt aagcgcctga
     5941 gcctggtgga ccctgcaaag cacccacaaa agaagcaaga caataagtgg aaacgccgca
     6001 aggaaggtat tgcgacgttc attgagcaga agaatgggtc ttactatttt aacgtggtca
     6061 ccaacaatgg ctgcaccagc tttcacttgt ggcacaagcc agacaacttc gaccaagaga
     6121 agttagaggg catccaaaac ggggagaagt tagattgctg ggttcgtgat agccgttacc
     6181 aaaaggcatt tcaagaaatc ccggagaacg atccggacgg atgggagtgt aaggaaggtt
     6241 atctgcatgt ggtgggccct tcgaaggttg aatttagcga caagaagggc gatgtcatta
     6301 ataacttcca agggactctc ccatcagtgc ccaatgattg gaaaacgatt cgtacaaacg
     6361 actttaagaa ccgcaaacgt aagaacgagc ccgttttctg ttgcgaggat gacaagggga
     6421 actactacac gatggccaaa tattgtgaaa cgttcttctt cgacctgaaa gagaacgagg
     6481 agtatgagat ccccgagaag gcacatatca agtacaagga gttattacgc gtgtacaaca
     6541 acaatccgca agcggttccc gagagcgtgt tccagagtcg cgtagcgcgc gagaacgtag
     6601 aaaagctgaa gtctggggat ttggtgtatt tcaagcacaa cgagaaatat gttgaggaca
     6661 tcgtaccggt tcgtatttca cgtaccgtag acgaccgcat gatcggaaag cgtatgtcgg
     6721 ctgatttgcg tccatgtcac ggtgactggg tagaggacgg ggacctctct gcgttgaatg
     6781 catatccaga gaagcgcctt ttattgcgtc accctaaggg cctttgcccg gcatgtcgcc
     6841 ttttcggaac gggtagctac aagggccgcg ttcgttttgg cttcgcgagc ctggagaacg
     6901 acccggagtg gttgattcct ggaaagaatc ccggtgatcc attccacggc gggccagtga
     6961 tgctgagctt gctggagcgc ccgcgtccca cctggagcat tcctggcagt gataataagt
     7021 tcaaagttcc aggccgtaag ttctacgttc accatcatgc ttggaaaacg attaaggacg
     7081 gtaaccatcc gaccaccggc aaagccattg aacaatcgcc aaataaccgc acggttgaag
     7141 cgctcgccgg tggcaacagc ttcagcttcg aaattgcgtt cgaaaatttg aaggagtggg
     7201 agttgggcct gctgatccat agcctgcagc ttgaaaaggg cttggcccat aagctcggta
     7261 tggctaagag catgggcttt ggctcagtag agatcgacgt tgagagtgtc cgcctgcgta
     7321 aagattggaa gcagtggcgc aatggtaatt cagagatccc aaattggttg ggcaaggggt
     7381 ttgcaaagct gaaggagtgg ttccgcgacg agctggactt catcgagaat ttgaagaaat
     7441 tactgtggtt ccccgaaggt gatcaagctc cgcgcgtatg ttaccccatg cttcgcaaga
     7501 aggatgaccc taatggaaac tcaggttatg aggagttgaa agacggcgaa tttaagaagg
     7561 aagatcgtca gaagaagctt acaacaccat ggacgccgtg ggctgcggcc gcactcgagc
     7621 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     7681 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     7741 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     7801 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7861 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7921 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7981 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8041 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8101 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8161 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8221 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
     8341 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     8401 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     8461 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     8521 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     8581 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     8641 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     8701 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     8761 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     8821 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     8881 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     8941 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     9001 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     9061 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     9121 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     9181 agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     9421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9961 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10021 cagtgagcga ggaagcggaa g
//
