LOCUS       sequence-468898-        8163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for bacterial expression and purification of High-fidelity 
            RfxCas13d protein (human codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia
            PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador 
            G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, 
            Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA,
            Moreno-Mateos MA
  TITLE     Enhanced RNA-targeting CRISPR-Cas technology in zebrafish.
  JOURNAL   Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9.
  PUBMED    40091120
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-03-16"; volume: "16(1):2591. doi"; pages: " 
            10.1038/s41467-025-57792-9"
COMMENT     Sequence Label: pET28b-Hf-RfxCas13d-His
FEATURES             Location/Qualifiers
     source          1..8163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     230..252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(246..437)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1246..2328)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2290..2309
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2329..2406)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2612..2631
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2715..2734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2715..2733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2734..2758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2773..2795
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2820..5717
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILVEYITNVVYVVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5742..5759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5812..5830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5826..5873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5910..6365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5997..6016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6207..6228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6457..7272)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6457..7272)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7180..7199
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7264..7283
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7394..7982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7883..7902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8136..8153
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
      301 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
      361 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      421 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
      481 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      541 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      601 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      661 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
      721 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
      781 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
      841 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
      901 cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      961 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     1021 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     1081 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     1141 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     1201 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     1261 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1321 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     1381 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     1441 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     1501 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     1561 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     1621 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     1681 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     1741 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     1801 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     1861 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     1921 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     1981 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     2041 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     2101 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     2161 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     2221 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     2281 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     2341 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     2401 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     2461 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     2521 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     2581 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     2641 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     2701 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     2761 tctagaaata attttgttta actttaagaa ggagatatac catggcgagc gaggccagca
     2821 tcgaaaaaaa aaagtccttc gccaagggca tgggcgtgaa gtccacactc gtgtccggct
     2881 ccaaagtgta catgacaacc ttcgccgaag gcagcgacgc caggctggaa aagatcgtgg
     2941 agggcgacag catcaggagc gtgaatgagg gcgaggcctt cagcgctgaa atggccgata
     3001 aaaacgccgg ctataagatc ggcaacgcca aattcagcca tcctaagggc tacgccgtgg
     3061 tggctaacaa ccctctgtat acaggacccg tccagcagga tatgctcggc ctgaaggaaa
     3121 ctctggaaaa gaggtacttc ggcgagagcg ctgatggcaa tgacaatatt tgtatccagg
     3181 tgatccataa catcctggac attgaaaaaa tcctcgtcga atacattacc aacgtggtct
     3241 acgtcgtcaa caatatctcc ggcctggata aggacattat tggattcggc aagttctcca
     3301 cagtgtatac ctacgacgaa ttcaaagacc ccgagcacca tagggccgct ttcaacaata
     3361 acgataagct catcaacgcc atcaaggccc agtatgacga gttcgacaac ttcctcgata
     3421 accccagact cggctatttc ggccaggcct ttttcagcaa ggagggcaga aattacatca
     3481 tcaattacgg caacgaatgc tatgacattc tggccctcct gagcggactg aggcactggg
     3541 tggtccataa caacgaagaa gagtccagga tctccaggac ctggctctac aacctcgata
     3601 agaacctcga caacgaatac atctccaccc tcaactacct ctacgacagg atcaccaatg
     3661 agctgaccaa ctccttctcc aagaactccg ccgccaacgt gaactatatt gccgaaactc
     3721 tgggaatcaa ccctgccgaa ttcgccgaac aatatttcag attcagcatt atgaaagagc
     3781 agaaaaacct cggattcaat atcaccaagc tcagggaagt gatgctggac aggaaggata
     3841 tgtccgagat caggaaaaat cataaggtgt tcgactccat caggaccaag gtctacacca
     3901 tgatggactt tgtgatttat aggtattaca tcgaagagga tgccaaggtg gctgccgcca
     3961 ataagtccct ccccgataat gagaagtccc tgagcgagaa ggatatcttt gtgattaacc
     4021 tgaggggctc cttcaacgac gaccagaagg atgccctcta ctacgatgaa gctaatagaa
     4081 tttggagaaa gctcgaaaat atcatgcaca acatcaagga atttagggga aacaagacaa
     4141 gagagtataa gaagaaggac gcccctagac tgcccagaat cctgcccgct ggccgtgatg
     4201 tttccgcctt cagcaaactc atgtatgccc tgaccatgtt cctggatggc aaggagatca
     4261 acgacctcct gaccaccctg attaataaat tcgataacat ccagagcttc ctgaaggtga
     4321 tgcctctcat cggagtcaac gctaagttcg tggaggaata cgcctttttc aaagactccg
     4381 ccaagatcgc cgatgagctg aggctgatca agtccttcgc tagaatggga gaacctattg
     4441 ccgatgccag gagggccatg tatatcgacg ccatccgtat tttaggaacc aacctgtcct
     4501 atgatgagct caaggccctc gccgacacct tttccctgga cgagaacgga aacaagctca
     4561 agaaaggcaa gcacggcatg agaaatttca ttattaataa cgtgatcagc aataaaaggt
     4621 tccactacct gatcagatac ggtgatcctg cccacctcca tgagatcgcc aaaaacgagg
     4681 ccgtggtgaa gttcgtgctc ggcaggatcg ctgacatcca gaaaaaacag ggccagaacg
     4741 gcaagaacca gatcgacagg tactacgaaa cttgtatcgg aaaggataag ggcaagagcg
     4801 tgagcgaaaa ggtggacgct ctcacaaaga tcatcaccgg aatgaactac gaccaattcg
     4861 acaagaaaag gagcgtcatt gaggacaccg gcagggaaaa cgccgagagg gagaagttta
     4921 aaaagatcat cagcctgtac ctcaccgtga tctaccacat cctcaagaat attgtcaata
     4981 tcaacgccag gtacgtcatc ggattccatt gcgtcgagcg tgatgctcaa ctgtacaagg
     5041 agaaaggcta cgacatcaat ctcaagaaac tggaagagaa gggattcagc tccgtcacca
     5101 agctctgcgc tggcattgat gaaactgccc ccgataagag aaaggacgtg gaaaaggaga
     5161 tggctgaaag agccaaggag agcattgaca gcctcgagag cgccaacccc aagctgtatg
     5221 ccaattacat caaatacagc gacgagaaga aagccgagga gttcaccagg cagattaaca
     5281 gggagaaggc caaaaccgcc ctgaacgcct acctgaggaa caccaagtgg aatgtgatca
     5341 tcagggagga cctcctgaga attgacaaca agacatgtac cctgttcaga aacaaggccg
     5401 tccacctgga agtggccagg tatgtccacg cctatatcaa cgacattgcc gaggtcaatt
     5461 cctacttcca actgtaccat tacatcatgc agagaattat catgaatgag aggtacgaga
     5521 aaagcagcgg aaaggtgtcc gagtacttcg acgctgtgaa tgacgagaag aagtacaacg
     5581 ataggctcct gaaactgctg tgtgtgcctt tcggctactg tatccccagg tttaagaacc
     5641 tgagcatcga ggccctgttc gataggaacg aggccgccaa gttcgacaag gagaaaaaga
     5701 aggtgtccgg caattccgga tccggagcgg ccgcactcga gcaccaccac caccaccact
     5761 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     5821 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     5881 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6361 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6421 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     6481 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     6541 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     6601 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     6661 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     6721 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     6781 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     6841 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     6901 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     6961 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     7021 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     7081 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     7141 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     7201 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     7261 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     7321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7501 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8161 aag
//
