LOCUS       sequence-468914-       13238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for integration at the native URA3 locus with 3xmCherry 
            under control of the ScACT1 promoter and 3xGFP under control of the 
            ApACT1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Colarusso AV, Williams AM, Gladfelter AS, Wirshing ACE, Lew DJ
  TITLE     Optimized vectors for genetic engineering of Aureobasidium 
            pullulans.
  JOURNAL   Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi: 10.1091/mbc.E25-02-0059. 
            Epub 2025 Apr 9.
  PUBMED    40202829
REFERENCE   2  (bases 1 to 13238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2025-06-1"; volume: "36(6):mr5. doi"; pages: " 
            10.1091/mbc.E25-02-0059. Epub 2025 Apr 9"
COMMENT     Sequence Label: pAP-U2-3
FEATURES             Location/Qualifiers
     source          1..13238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..150
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      6188..6375
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6243..6267)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7741..7761)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7741..7759)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7780..7796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7780..7796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7793..7815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7804..7820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7828..7834,7835..7852,7853..7858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8011..8028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8182..8770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8262..8281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8941..9732,9733..9801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9564..9583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9802..9906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9933..10389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10020..10039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10230..10251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10515..10537
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10529..10546
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10530..10546
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10556..10575
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10556..10574
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      13006..13203
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aaaaaaaaga aaaatttgaa atataaataa cgttcttaat actaacataa ctattaaaaa
       61 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
      121 gtgggaggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc gacaaaggaa
      181 aaggggcctg attgttaact cttgtagagc tcgtccatgc cgccggtaga gtggcggccc
      241 tcggcgcgct cgtattgctc gacgatggtg tagtcctcgt tgtgagaggt gatgtcgagc
      301 ttgatgttga cgttgtaggc gccagggagt tggacaggct tcttggcctt gtaggtggtc
      361 ttgacctcgg cgtcgtagtg gccgccgtcc ttgagcttga ggcgttgctt gatctcgccc
      421 ttgagggcgc cgtcctcagg gtacatgcgc tcagaagagg cctcccagcc catggtcttc
      481 ttttgcatga cagggccgtc agaagggaag ttggtgccgc ggagcttgac cttgtagatg
      541 aactcgccgt cttggagaga agagtcttgg gtgacggtga cgacgccgcc gtcctcgaag
      601 ttcatgacgc gctcccactt gaagccctca gggaaagaga gcttgaggta gtcagggatg
      661 tcggcagggt gcttgacgta ggccttagag ccgtacatga attgaggaga gaggatgtcc
      721 caggcgaaag ggagagggcc gcccttggtg accttgagct tggcggtttg ggtgccctcg
      781 taagggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttgac agagccctcc
      841 atgtggacct tgaagcgcat gaactccttg atgatggcca tgttgtcctc ctcgccctta
      901 gagaccatgc cagcagagcc agcagacttg tagagctcgt ccatgccgcc ggtagagtgg
      961 cggccctcgg cgcgctcgta ttgctcgacg atggtgtagt cctcgttgtg agaggtgatg
     1021 tcgagcttga tgttgacgtt gtaggcgcca gggagttgga caggcttctt ggccttgtag
     1081 gtggtcttga cctcggcgtc gtagtggccg ccgtccttga gcttgaggcg ttgcttgatc
     1141 tcgcccttga gggcgccgtc ctcagggtac atgcgctcag aagaggcctc ccagcccatg
     1201 gtcttctttt gcatgacagg gccgtcagaa gggaagttgg tgccgcggag cttgaccttg
     1261 tagatgaact cgccgtcttg gagagaagag tcttgggtga cggtgacgac gccgccgtcc
     1321 tcgaagttca tgacgcgctc ccacttgaag ccctcaggga aagagagctt gaggtagtca
     1381 gggatgtcgg cagggtgctt gacgtaggcc ttagagccgt acatgaattg aggagagagg
     1441 atgtcccagg cgaaagggag agggccgccc ttggtgacct tgagcttggc ggtttgggtg
     1501 ccctcgtaag ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttgacagag
     1561 ccctccatgt ggaccttgaa gcgcatgaac tccttgatga tggccatgtt gtcctcctcg
     1621 cccttagaga ccatgccaga gccgccagag cccttgtaga gctcgtccat gccgccggta
     1681 gagtggcggc cctcggcgcg ctcgtattgc tcgacgatgg tgtagtcctc gttgtgagag
     1741 gtgatgtcga gcttgatgtt gacgttgtag gcgccaggga gttggacagg cttcttggcc
     1801 ttgtaggtgg tcttgacctc ggcgtcgtag tggccgccgt ccttgagctt gaggcgttgc
     1861 ttgatctcgc ccttgagggc gccgtcctca gggtacatgc gctcagaaga ggcctcccag
     1921 cccatggtct tcttttgcat gacagggccg tcagaaggga agttggtgcc gcggagcttg
     1981 accttgtaga tgaactcgcc gtcttggaga gaagagtctt gggtgacggt gacgacgccg
     2041 ccgtcctcga agttcatgac gcgctcccac ttgaagccct cagggaaaga gagcttgagg
     2101 tagtcaggga tgtcggcagg gtgcttgacg taggccttag agccgtacat gaattgagga
     2161 gagaggatgt cccaggcgaa agggagaggg ccgcccttgg tgaccttgag cttggcggtt
     2221 tgggtgccct cgtaagggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttg
     2281 acagagccct ccatgtggac cttgaagcgc atgaactcct tgatgatggc catgttgtcc
     2341 tcctcgccct tagagaccat gtttaattaa tgttaattca gtaaattttc gatcttggga
     2401 agaaaaaagc agtaagcgtg aaaaatctaa aagctgatgt agtagaagat cctattcttt
     2461 aacaaagatt gaccttttct ttttcttctt ggtttgagta gaaaggggaa ggaagaatac
     2521 aagagagagg aaaaaaagga agataaaaag agagcgtgat ataaatgaat atatattaaa
     2581 caagagagat tgggaaggaa aggatcaaac aaacccaaaa atatttcaaa aaggagagag
     2641 agaggcgagt ttggtttcaa aacggtttat ttatttatgc aagaggacgt ggaagaaaaa
     2701 gaagaaggaa gaaaaaaatt tgaaagaaaa aaacgcgtgg cgggtaaaga agaaaatgga
     2761 aaatagaggc cgggtgacag agaaatattg agggttaatt ggaaaatatg ttagggtgag
     2821 gcatatgttt ttaagggttt tgaagatccg ataaggaaga atgtaggtta aatgttgtgc
     2881 attaattgct gtggcagctt acccgcttcc ccacacattt tggtagtatc tgtcctcttg
     2941 ttattgttac tgtaattgtg tatatatgtt cccagcgctg ggggcctaca cttggccacc
     3001 acgcgctggg ctactgtgag gtgacatatc tgtcttctcg attccccatt aagttcgcgt
     3061 tggccgtcgt caaatccaat agataagaag tggcacgtct acgtctttgg cgcaacctac
     3121 cttgatgatg ttcggccgga ttctccatga ttattcaaca gaacatcgtc tttggacatc
     3181 acacgctcag attgatggct gggatttcgt tggttgtcta gccatggtat tgctggaaac
     3241 cgtttgggcg gatcgcaact ccagcccgtg atcatcggcg cttgaacgtt gccggcaccg
     3301 gcaagaggct ccgcgaagct ctttatcgta gtggggaagc gatttgtttt cttttttctg
     3361 catgtccaga aagtcacgat caatgacaag gaggcgtttg atagagtcca agccaaatca
     3421 gagagtcgaa atgggagcat ttcccatatt gaagcaggcc gcgagattgt gctagatgga
     3481 atacggagcg tcgtatcgaa aggggccctg ggaaagtggc agactggcag aaccccactc
     3541 cgcgttcaac aatccttcaa gcgccgagat ggtcccaaag ggctggcaga gcgattgctg
     3601 atggggacac cgttcgacaa tcgaggctgg gactgtggtc attcatctgg ctcgacgcgt
     3661 tcagacacga aggaagttga ggattgaaga aagagactcg ggagagggca caacgtctgc
     3721 ccgccatgca gcatcgctcg cgcttggcat gatggctgtg gatgatgtat tcgagagctt
     3781 actcagagtc tcggtcctgt gccacgcgtc ctgcacaaca cagtcaaggc agccgtattt
     3841 ggcaagaggt atataggcag gagcgagagc agaggcagga ttgcaagtag acaatcattt
     3901 gcggaaacaa caggacgact cgtcaagcac acgcaacggg gctctggctg ttgataaccg
     3961 gaggcttaga cattgacaat tctgacctta agatgtctaa gggcgaggag ctcttcaccg
     4021 gcgtcgtccc tatcctcgtc gagctcgacg gcgacgtcaa cggccacaag ttctctgtct
     4081 ctggcgaggg cgagggcgac gccacctacg gcaagctcac cctcaagttc atctgcacca
     4141 ccggcaagct ccctgtccct tggcctaccc tcgtcaccac cttcggctac ggcgtccaat
     4201 gcttcgcccg ctaccctgac cacatgaagc aacacgactt cttcaagtct gccatgcctg
     4261 agggctacgt ccaagagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4321 ccgaggtcaa gttcgagggc gacaccctcg tcaaccgcat cgagctcaag ggcatcgact
     4381 tcaaggagga cggcaacatc ctcggccaca agctcgagta caactacaac tctcacaacg
     4441 tctacatcat ggccgacaag caaaagaacg gcatcaaggt caacttcaag atccgccaca
     4501 acatcgagga cggctctgtc caactcgccg accactacca acaaaacacc cctatcggcg
     4561 acggccctgt cctcctccct gacaaccact acctctctac ccaatctgcc ctctctaagg
     4621 accctaacga gaagcgcgac cacatggtcc tcctcgagtt cgtcaccgcc gccggcatca
     4681 cccacggcat ggacgagctc tacaagggct ctggcggctc tggcatgtct aagggcgagg
     4741 agctcttcac cggcgtcgtc cctatcctcg tcgagctcga cggcgacgtc aacggccaca
     4801 agttctctgt ctctggcgag ggcgagggcg acgccaccta cggcaagctc accctcaagt
     4861 tcatctgcac caccggcaag ctccctgtcc cttggcctac cctcgtcacc accttcggct
     4921 acggcgtcca atgcttcgcc cgctaccctg accacatgaa gcaacacgac ttcttcaagt
     4981 ctgccatgcc tgagggctac gtccaagagc gcaccatctt cttcaaggac gacggcaact
     5041 acaagacccg cgccgaggtc aagttcgagg gcgacaccct cgtcaaccgc atcgagctca
     5101 agggcatcga cttcaaggag gacggcaaca tcctcggcca caagctcgag tacaactaca
     5161 actctcacaa cgtctacatc atggccgaca agcaaaagaa cggcatcaag gtcaacttca
     5221 agatccgcca caacatcgag gacggctctg tccaactcgc cgaccactac caacaaaaca
     5281 cccctatcgg cgacggccct gtcctcctcc ctgacaacca ctacctctct acccaatctg
     5341 ccctctctaa ggaccctaac gagaagcgcg accacatggt cctcctcgag ttcgtcaccg
     5401 ccgccggcat cacccacggc atggacgagc tctacaagtc tgctggctct gctggcatgt
     5461 ctaagggcga ggagctcttc accggcgtcg tccctatcct cgtcgagctc gacggcgacg
     5521 tcaacggcca caagttctct gtctctggcg agggcgaggg cgacgccacc tacggcaagc
     5581 tcaccctcaa gttcatctgc accaccggca agctccctgt cccttggcct accctcgtca
     5641 ccaccttcgg ctacggcgtc caatgcttcg cccgctaccc tgaccacatg aagcaacacg
     5701 acttcttcaa gtctgccatg cctgagggct acgtccaaga gcgcaccatc ttcttcaagg
     5761 acgacggcaa ctacaagacc cgcgccgagg tcaagttcga gggcgacacc ctcgtcaacc
     5821 gcatcgagct caagggcatc gacttcaagg aggacggcaa catcctcggc cacaagctcg
     5881 agtacaacta caactctcac aacgtctaca tcatggccga caagcaaaag aacggcatca
     5941 aggtcaactt caagatccgc cacaacatcg aggacggctc tgtccaactc gccgaccact
     6001 accaacaaaa cacccctatc ggcgacggcc ctgtcctcct ccctgacaac cactacctct
     6061 ctacccaatc tgccctctct aaggacccta acgagaagcg cgaccacatg gtcctcctcg
     6121 agttcgtcac cgccgccggc atcacccacg gcatggacga gctctacaag ggtggatcct
     6181 aggcgccgcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     6241 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     6301 taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg ctcttattga
     6361 ccacacctct accggcagat ccgctaggga taacagggta atacatatca ctgacatttg
     6421 agaagctggg atattgttca ggcaggacga ggatgtgtgc ttttctacgg tcaggaaccg
     6481 acaggaaaca tcgaagcctc cgatacggag gacagactgg aaactgaatt gaggatcata
     6541 tttggaggac tactttttca aggcgcatgt tggagtgaag atacaaaaaa gtgtagagca
     6601 gcttgggatt catccatcat caccatcatc ttcataatca ctttacctcc gtctcaactc
     6661 atgagttggt ccgattgacc ttgggagctg actaccaaca atggttcgga tcgcacggca
     6721 caagatttga ttgatcagcg agctggtccc tgatgtgtcc ggatgatttg tgttgtatac
     6781 ggagggctcc acaatgattg agaaacggca agacttgctg caagattcgg cactcacgat
     6841 cgaccatgcg agaagagctt gtaggccgct gaacgaattg ggtgatatcg cctacccatt
     6901 taagaacagt gacgaagaca tcacaagcaa accatattcg aagatccggt ttgagtgaat
     6961 gaatcaaaaa agatgaaagg gacgtaggcg tgaacatgcg ttaagcgggc tttgaaagtg
     7021 cgtctccctc tctttctctt ttatgcctcg atagctgttg aacactctct cgcctgcaat
     7081 cacctacttc attctgctct tctacaagaa gccggtccaa acaagtggaa gcccataagg
     7141 atatatggac gtgacccgta tgatagaaca atgttatgct cggtttcgat tctgtcaaac
     7201 tatggatatg tggttgagct atgtctaggc tgcccaaagc ctctcgttag ccatgaacag
     7261 gtctctggta tcaagatgtt tgaacaaatg actttgagct tgtcgattct tgtagtgttg
     7321 gtgcatgcta gctccagcag aatctttctt ccataccaac ggtttcttga cctttgacca
     7381 agcatagtca tgtctgcact cctgccagca ccaccaacat acgccgactt cacctagagc
     7441 aagaaccaag agatctaata gaggcaggat gcgtgtgcga atgccacagt gatgatggtt
     7501 tttgtctacc tgatactcgg gagcttgttt gaagctgtcg acaagatcta ggacgtcgcc
     7561 atcgtagggt tcaggcggat ctgaggttgc aaagatggtg ctttgcaagg aaagagtgtt
     7621 gatacgtttg aagaatttga tggtctgacc caattggaac aaatcgcatt cgggcgagga
     7681 gtcgtagccc agcttacact gtcgctcgcg agacgtgtat aggagctcca gcttttgttc
     7741 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9901 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     9961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    10021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    10081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10141 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10261 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10321 gttaaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10381 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10441 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10501 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
    10561 cgactcacta tagggcgaat tgggtaccgg gccctgagtg ttggtgtacg cgcatacaca
    10621 atgatccgcg gaaggatgtt ccccctgcgg tggcacagaa caggcaatct aacaagctcg
    10681 cccttgctgc aaggcacaga ggagctgaag ccggcgcatg attgttgaag atctccaggt
    10741 cttcgacagc aagcataaag gcaaaacgaa acgggcgcag agtcgcaaac cgagagctgc
    10801 ggagggacaa gttgagcgac aagcgggggc aggaattgac gagcttcctc catgcacgtc
    10861 tcgcggaccg gaagctgctg cgacccacac gggtgtcgtt gggactggcc tagaaaggat
    10921 ttacatggct tggcttgggc tggcaaggaa gactccgaag agtggagtgt tctcaggcag
    10981 ttcagagtca agatcaggaa ggtgcagaca gtgaagagga gtgttgatgg atgcagggat
    11041 cgctagcagg gctgcgatag acggggaaga gcgaatccga gcagaggtgg tctctgaggt
    11101 cgcagtgcac tacctgtcaa tggccctaga ccctgcattg gcgtgcgact gcggtcaagc
    11161 agagtcttgt gttttggtgg acacttgcgg cctcgaatat gcagcagcgg caggtttcaa
    11221 gcagcatccg aagccctcgg gtcccacccc acctctggag gcttttgagc agcaatggca
    11281 ggtggcagga gtcggaagag cgaaactggc tcccgcacgc tgcttgtcag aaggaagcat
    11341 aaagatggac atgtagacag cagcctcgct ctttgctgct ttcctttccc aactgccaga
    11401 gcctccccca gcctgaccgt ccttgagttg tggcttgttg acttgttgac ttgttgactt
    11461 gttgacttgt gattgtgtac tgcacatctc cggccgtgtc ctctcgcttt gacttgctcg
    11521 gcgcaaaatc tcgactgctc tcagtggtct gggctggtgc tcctcggtcg tcatttaaca
    11581 ttttgcttgc aacttcccat ctggtgcttc ttgcgtacaa gcatccttcc ctctcaattg
    11641 ccttgatcca tagctattcg acagctaccc agtcgcccag cctcccgcat ctcttgaccg
    11701 gcttcgtcca cgcacctccc acactcccac aaagtctcgc tcgacgtcga cgcccgcaat
    11761 gtcccgtcac gcaacaatca cccagtcata ccaggagcga gcctcgctgc ccaagacatc
    11821 accggtcgcc tcgtacctcc tacgattgat cgcagcgaag cagacaaatc tctgtgtctc
    11881 ggcagatgtg tcgacaacca gagagctgct gcaactcgcc gaagaggtcg gcgactcaat
    11941 atgcatgctc aagacacacg ccgacatcat taatgacttt ggccctcgca ccatcgaggg
    12001 cctgaaagag attgctgcca agaagcactt cctcgtcttc gaggaccgca agtttggcga
    12061 cattggcagt aggtgcctgc cccacccgtg tcttccaatc accgaccact gacacagctt
    12121 cttgcaggta ccgtgcaaaa gcagttcact gccggccctc tgcagatcgt tcgctgggcc
    12181 aatatcatca atgcccacat cttccctggc cccgccatca tcactgctct ctcgcaagcc
    12241 gcccacgatg ccgtcagttc tctcaacact gctgtcacca cttccatctc ggcctcgccc
    12301 gttccctcat acatggacga cagcgacgaa gtagactcgc ctgcactgtc tcaccacgaa
    12361 gactcagacg atctcgacag actctcgagc gacgatgact ccaaccaatt gtctacatac
    12421 agtgacccca ctggtcgcaa gccaagcgtc gtttccgtat ctaccactat cagcaccaag
    12481 acagaaagca tctcacccca gcctacgcca aaccaccacg gtggccccaa cgattctatt
    12541 tcgtccatca gcgccggctc ggctggtcaa gaatcgtctg ctttggcccg cctcggagag
    12601 ccccctctcc tccgaagctt gctgatcctc gcagagatga gcagtgcagg caacttgatg
    12661 actggctcat acactgaaca atgtgttgtg gaagcacgca agaacccgga gttcgtcatg
    12721 ggcttcatcg cacaacgcac tctcaatgag cagcctggtg acaatttcat caccatgact
    12781 cctggcgtcc agattggcgc cactggagat ggtctcggcc aacagtacaa cactccggaa
    12841 aaggtcatcg gcgaagctgg caccgacatc atcattgtcg gccgcggtgt ctacggtgct
    12901 caagataaga gggccaaggc cgaggaatac agagtcagag catggaaggc atacgagggg
    12961 agaattggcg ctgctactgc aacaaaaggt agcaggtgag aattcactga caataaaaag
    13021 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
    13081 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
    13141 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
    13201 ctgcccggga tgttacatgc gtacacgcgt ttgtacag
//
