LOCUS       sequence-468916-       12021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for integration at the native URA3 locus with 3xmCherry and 
            3xGFP under control of the strong bidirectional SpH2A/B promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Colarusso AV, Williams AM, Gladfelter AS, Wirshing ACE, Lew DJ
  TITLE     Optimized vectors for genetic engineering of Aureobasidium 
            pullulans.
  JOURNAL   Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi: 10.1091/mbc.E25-02-0059. 
            Epub 2025 Apr 9.
  PUBMED    40202829
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2025-06-1"; volume: "36(6):mr5. doi"; pages: " 
            10.1091/mbc.E25-02-0059. Epub 2025 Apr 9"
COMMENT     Sequence Label: pAP-U2-1
FEATURES             Location/Qualifiers
     source          1..12021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..36)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1510..1530)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1510..1528)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1549..1565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1549..1565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1562..1584)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1573..1589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1597..1603,1604..1621,1622..1627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1642..1663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1780..1797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1951..2539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2031..2050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2710..3501,3502..3570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3333..3352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3571..3675)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3702..4158
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3789..3808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3999..4020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4284..4306
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4298..4315
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4299..4315
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4325..4344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4325..4343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6775..6972
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     7139..7157
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      join(11978..12021,1..144)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
ORIGIN
        1 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
       61 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc
      121 tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa tacatatcac
      181 tgacatttga gaagctggga tattgttcag gcaggacgag gatgtgtgct tttctacggt
      241 caggaaccga caggaaacat cgaagcctcc gatacggagg acagactgga aactgaattg
      301 aggatcatat ttggaggact actttttcaa ggcgcatgtt ggagtgaaga tacaaaaaag
      361 tgtagagcag cttgggattc atccatcatc accatcatct tcataatcac tttacctccg
      421 tctcaactca tgagttggtc cgattgacct tgggagctga ctaccaacaa tggttcggat
      481 cgcacggcac aagatttgat tgatcagcga gctggtccct gatgtgtccg gatgatttgt
      541 gttgtatacg gagggctcca caatgattga gaaacggcaa gacttgctgc aagattcggc
      601 actcacgatc gaccatgcga gaagagcttg taggccgctg aacgaattgg gtgatatcgc
      661 ctacccattt aagaacagtg acgaagacat cacaagcaaa ccatattcga agatccggtt
      721 tgagtgaatg aatcaaaaaa gatgaaaggg acgtaggcgt gaacatgcgt taagcgggct
      781 ttgaaagtgc gtctccctct ctttctcttt tatgcctcga tagctgttga acactctctc
      841 gcctgcaatc acctacttca ttctgctctt ctacaagaag ccggtccaaa caagtggaag
      901 cccataagga tatatggacg tgacccgtat gatagaacaa tgttatgctc ggtttcgatt
      961 ctgtcaaact atggatatgt ggttgagcta tgtctaggct gcccaaagcc tctcgttagc
     1021 catgaacagg tctctggtat caagatgttt gaacaaatga ctttgagctt gtcgattctt
     1081 gtagtgttgg tgcatgctag ctccagcaga atctttcttc cataccaacg gtttcttgac
     1141 ctttgaccaa gcatagtcat gtctgcactc ctgccagcac caccaacata cgccgacttc
     1201 acctagagca agaaccaaga gatctaatag aggcaggatg cgtgtgcgaa tgccacagtg
     1261 atgatggttt ttgtctacct gatactcggg agcttgtttg aagctgtcga caagatctag
     1321 gacgtcgcca tcgtagggtt caggcggatc tgaggttgca aagatggtgc tttgcaagga
     1381 aagagtgttg atacgtttga agaatttgat ggtctgaccc aattggaaca aatcgcattc
     1441 gggcgaggag tcgtagccca gcttacactg tcgctcgcga gacgtgtata ggagctccag
     1501 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     1561 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     3721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3901 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4021 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4081 ggcctattgg ttaaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4141 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     4201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     4261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
     4321 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccctgagtgt tggtgtacgc
     4381 gcatacacaa tgatccgcgg aaggatgttc cccctgcggt ggcacagaac aggcaatcta
     4441 acaagctcgc ccttgctgca aggcacagag gagctgaagc cggcgcatga ttgttgaaga
     4501 tctccaggtc ttcgacagca agcataaagg caaaacgaaa cgggcgcaga gtcgcaaacc
     4561 gagagctgcg gagggacaag ttgagcgaca agcgggggca ggaattgacg agcttcctcc
     4621 atgcacgtct cgcggaccgg aagctgctgc gacccacacg ggtgtcgttg ggactggcct
     4681 agaaaggatt tacatggctt ggcttgggct ggcaaggaag actccgaaga gtggagtgtt
     4741 ctcaggcagt tcagagtcaa gatcaggaag gtgcagacag tgaagaggag tgttgatgga
     4801 tgcagggatc gctagcaggg ctgcgataga cggggaagag cgaatccgag cagaggtggt
     4861 ctctgaggtc gcagtgcact acctgtcaat ggccctagac cctgcattgg cgtgcgactg
     4921 cggtcaagca gagtcttgtg ttttggtgga cacttgcggc ctcgaatatg cagcagcggc
     4981 aggtttcaag cagcatccga agccctcggg tcccacccca cctctggagg cttttgagca
     5041 gcaatggcag gtggcaggag tcggaagagc gaaactggct cccgcacgct gcttgtcaga
     5101 aggaagcata aagatggaca tgtagacagc agcctcgctc tttgctgctt tcctttccca
     5161 actgccagag cctcccccag cctgaccgtc cttgagttgt ggcttgttga cttgttgact
     5221 tgttgacttg ttgacttgtg attgtgtact gcacatctcc ggccgtgtcc tctcgctttg
     5281 acttgctcgg cgcaaaatct cgactgctct cagtggtctg ggctggtgct cctcggtcgt
     5341 catttaacat tttgcttgca acttcccatc tggtgcttct tgcgtacaag catccttccc
     5401 tctcaattgc cttgatccat agctattcga cagctaccca gtcgcccagc ctcccgcatc
     5461 tcttgaccgg cttcgtccac gcacctccca cactcccaca aagtctcgct cgacgtcgac
     5521 gcccgcaatg tcccgtcacg caacaatcac ccagtcatac caggagcgag cctcgctgcc
     5581 caagacatca ccggtcgcct cgtacctcct acgattgatc gcagcgaagc agacaaatct
     5641 ctgtgtctcg gcagatgtgt cgacaaccag agagctgctg caactcgccg aagaggtcgg
     5701 cgactcaata tgcatgctca agacacacgc cgacatcatt aatgactttg gccctcgcac
     5761 catcgagggc ctgaaagaga ttgctgccaa gaagcacttc ctcgtcttcg aggaccgcaa
     5821 gtttggcgac attggcagta ggtgcctgcc ccacccgtgt cttccaatca ccgaccactg
     5881 acacagcttc ttgcaggtac cgtgcaaaag cagttcactg ccggccctct gcagatcgtt
     5941 cgctgggcca atatcatcaa tgcccacatc ttccctggcc ccgccatcat cactgctctc
     6001 tcgcaagccg cccacgatgc cgtcagttct ctcaacactg ctgtcaccac ttccatctcg
     6061 gcctcgcccg ttccctcata catggacgac agcgacgaag tagactcgcc tgcactgtct
     6121 caccacgaag actcagacga tctcgacaga ctctcgagcg acgatgactc caaccaattg
     6181 tctacataca gtgaccccac tggtcgcaag ccaagcgtcg tttccgtatc taccactatc
     6241 agcaccaaga cagaaagcat ctcaccccag cctacgccaa accaccacgg tggccccaac
     6301 gattctattt cgtccatcag cgccggctcg gctggtcaag aatcgtctgc tttggcccgc
     6361 ctcggagagc cccctctcct ccgaagcttg ctgatcctcg cagagatgag cagtgcaggc
     6421 aacttgatga ctggctcata cactgaacaa tgtgttgtgg aagcacgcaa gaacccggag
     6481 ttcgtcatgg gcttcatcgc acaacgcact ctcaatgagc agcctggtga caatttcatc
     6541 accatgactc ctggcgtcca gattggcgcc actggagatg gtctcggcca acagtacaac
     6601 actccggaaa aggtcatcgg cgaagctggc accgacatca tcattgtcgg ccgcggtgtc
     6661 tacggtgctc aagataagag ggccaaggcc gaggaataca gagtcagagc atggaaggca
     6721 tacgagggga gaattggcgc tgctactgca acaaaaggta gcaggtgaga attcactgac
     6781 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     6841 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     6901 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     6961 gtcgctatac tgcccgggat gttacatgcg tacacgcgtt tgtacagaaa aaaaagaaaa
     7021 atttgaaata taaataacgt tcttaatact aacataacta ttaaaaaaaa taaataggga
     7081 cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg ggaggagggc
     7141 gtgaatgtaa gcgtgacata actaattaca tgatatcgac aaaggaaaag gggcctgtct
     7201 acttgtagag ctcgtccatg ccgccggtag agtggcggcc ctcggcgcgc tcgtattgct
     7261 cgacgatggt gtagtcctcg ttgtgagagg tgatgtcgag cttgatgttg acgttgtagg
     7321 cgccagggag ttggacaggc ttcttggcct tgtaggtggt cttgacctcg gcgtcgtagt
     7381 ggccgccgtc cttgagcttg aggcgttgct tgatctcgcc cttgagggcg ccgtcctcag
     7441 ggtacatgcg ctcagaagag gcctcccagc ccatggtctt cttttgcatg acagggccgt
     7501 cagaagggaa gttggtgccg cggagcttga ccttgtagat gaactcgccg tcttggagag
     7561 aagagtcttg ggtgacggtg acgacgccgc cgtcctcgaa gttcatgacg cgctcccact
     7621 tgaagccctc agggaaagag agcttgaggt agtcagggat gtcggcaggg tgcttgacgt
     7681 aggccttaga gccgtacatg aattgaggag agaggatgtc ccaggcgaaa gggagagggc
     7741 cgcccttggt gaccttgagc ttggcggttt gggtgccctc gtaagggcgg ccctcgccct
     7801 cgccctcgat ctcgaactcg tggccgttga cagagccctc catgtggacc ttgaagcgca
     7861 tgaactcctt gatgatggcc atgttgtcct cctcgccctt agagaccatg ccagcagagc
     7921 cagcagactt gtagagctcg tccatgccgc cggtagagtg gcggccctcg gcgcgctcgt
     7981 attgctcgac gatggtgtag tcctcgttgt gagaggtgat gtcgagcttg atgttgacgt
     8041 tgtaggcgcc agggagttgg acaggcttct tggccttgta ggtggtcttg acctcggcgt
     8101 cgtagtggcc gccgtccttg agcttgaggc gttgcttgat ctcgcccttg agggcgccgt
     8161 cctcagggta catgcgctca gaagaggcct cccagcccat ggtcttcttt tgcatgacag
     8221 ggccgtcaga agggaagttg gtgccgcgga gcttgacctt gtagatgaac tcgccgtctt
     8281 ggagagaaga gtcttgggtg acggtgacga cgccgccgtc ctcgaagttc atgacgcgct
     8341 cccacttgaa gccctcaggg aaagagagct tgaggtagtc agggatgtcg gcagggtgct
     8401 tgacgtaggc cttagagccg tacatgaatt gaggagagag gatgtcccag gcgaaaggga
     8461 gagggccgcc cttggtgacc ttgagcttgg cggtttgggt gccctcgtaa gggcggccct
     8521 cgccctcgcc ctcgatctcg aactcgtggc cgttgacaga gccctccatg tggaccttga
     8581 agcgcatgaa ctccttgatg atggccatgt tgtcctcctc gcccttagag accatgccag
     8641 agccgccaga gcccttgtag agctcgtcca tgccgccggt agagtggcgg ccctcggcgc
     8701 gctcgtattg ctcgacgatg gtgtagtcct cgttgtgaga ggtgatgtcg agcttgatgt
     8761 tgacgttgta ggcgccaggg agttggacag gcttcttggc cttgtaggtg gtcttgacct
     8821 cggcgtcgta gtggccgccg tccttgagct tgaggcgttg cttgatctcg cccttgaggg
     8881 cgccgtcctc agggtacatg cgctcagaag aggcctccca gcccatggtc ttcttttgca
     8941 tgacagggcc gtcagaaggg aagttggtgc cgcggagctt gaccttgtag atgaactcgc
     9001 cgtcttggag agaagagtct tgggtgacgg tgacgacgcc gccgtcctcg aagttcatga
     9061 cgcgctccca cttgaagccc tcagggaaag agagcttgag gtagtcaggg atgtcggcag
     9121 ggtgcttgac gtaggcctta gagccgtaca tgaattgagg agagaggatg tcccaggcga
     9181 aagggagagg gccgcccttg gtgaccttga gcttggcggt ttgggtgccc tcgtaagggc
     9241 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt gacagagccc tccatgtgga
     9301 ccttgaagcg catgaactcc ttgatgatgg ccatgttgtc ctcctcgccc ttagagacca
     9361 tgtttaatta atgctgtgta aggtgtgcgt gagagagagg gtcgtgagtg agtcttgaag
     9421 agaaacgtca aagcttgaag cgtagcagaa tacggaaaac gacttggggg aatggcgagg
     9481 agcgtcgcct tttatgctcc aagcggagac gatgaggccg atattacagt ggcttagtag
     9541 cgtgtacaat atttccataa aaggctacat ggtgagtttt tcacgtgacc gtaattagtc
     9601 ccaattaggc tttattgtat attgctacta agcatcgtta aatgacctgc tcgaagcttc
     9661 gtctataaaa ggcgggcgtg tgtgcgcttc ccccgctacg tctttttcac aaacactttg
     9721 cgacttgacc aagacctttc acccgcacca ctctcatcca tcacacacac aaaaacctta
     9781 agatgtctaa gggcgaggag ctcttcaccg gcgtcgtccc tatcctcgtc gagctcgacg
     9841 gcgacgtcaa cggccacaag ttctctgtct ctggcgaggg cgagggcgac gccacctacg
     9901 gcaagctcac cctcaagttc atctgcacca ccggcaagct ccctgtccct tggcctaccc
     9961 tcgtcaccac cttcggctac ggcgtccaat gcttcgcccg ctaccctgac cacatgaagc
    10021 aacacgactt cttcaagtct gccatgcctg agggctacgt ccaagagcgc accatcttct
    10081 tcaaggacga cggcaactac aagacccgcg ccgaggtcaa gttcgagggc gacaccctcg
    10141 tcaaccgcat cgagctcaag ggcatcgact tcaaggagga cggcaacatc ctcggccaca
    10201 agctcgagta caactacaac tctcacaacg tctacatcat ggccgacaag caaaagaacg
    10261 gcatcaaggt caacttcaag atccgccaca acatcgagga cggctctgtc caactcgccg
    10321 accactacca acaaaacacc cctatcggcg acggccctgt cctcctccct gacaaccact
    10381 acctctctac ccaatctgcc ctctctaagg accctaacga gaagcgcgac cacatggtcc
    10441 tcctcgagtt cgtcaccgcc gccggcatca cccacggcat ggacgagctc tacaagggct
    10501 ctggcggctc tggcatgtct aagggcgagg agctcttcac cggcgtcgtc cctatcctcg
    10561 tcgagctcga cggcgacgtc aacggccaca agttctctgt ctctggcgag ggcgagggcg
    10621 acgccaccta cggcaagctc accctcaagt tcatctgcac caccggcaag ctccctgtcc
    10681 cttggcctac cctcgtcacc accttcggct acggcgtcca atgcttcgcc cgctaccctg
    10741 accacatgaa gcaacacgac ttcttcaagt ctgccatgcc tgagggctac gtccaagagc
    10801 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtc aagttcgagg
    10861 gcgacaccct cgtcaaccgc atcgagctca agggcatcga cttcaaggag gacggcaaca
    10921 tcctcggcca caagctcgag tacaactaca actctcacaa cgtctacatc atggccgaca
    10981 agcaaaagaa cggcatcaag gtcaacttca agatccgcca caacatcgag gacggctctg
    11041 tccaactcgc cgaccactac caacaaaaca cccctatcgg cgacggccct gtcctcctcc
    11101 ctgacaacca ctacctctct acccaatctg ccctctctaa ggaccctaac gagaagcgcg
    11161 accacatggt cctcctcgag ttcgtcaccg ccgccggcat cacccacggc atggacgagc
    11221 tctacaagtc tgctggctct gctggcatgt ctaagggcga ggagctcttc accggcgtcg
    11281 tccctatcct cgtcgagctc gacggcgacg tcaacggcca caagttctct gtctctggcg
    11341 agggcgaggg cgacgccacc tacggcaagc tcaccctcaa gttcatctgc accaccggca
    11401 agctccctgt cccttggcct accctcgtca ccaccttcgg ctacggcgtc caatgcttcg
    11461 cccgctaccc tgaccacatg aagcaacacg acttcttcaa gtctgccatg cctgagggct
    11521 acgtccaaga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
    11581 tcaagttcga gggcgacacc ctcgtcaacc gcatcgagct caagggcatc gacttcaagg
    11641 aggacggcaa catcctcggc cacaagctcg agtacaacta caactctcac aacgtctaca
    11701 tcatggccga caagcaaaag aacggcatca aggtcaactt caagatccgc cacaacatcg
    11761 aggacggctc tgtccaactc gccgaccact accaacaaaa cacccctatc ggcgacggcc
    11821 ctgtcctcct ccctgacaac cactacctct ctacccaatc tgccctctct aaggacccta
    11881 acgagaagcg cgaccacatg gtcctcctcg agttcgtcac cgccgccggc atcacccacg
    11941 gcatggacga gctctacaag ggtggatcct aggcgccgcg aatttcttat gatttatgat
    12001 ttttattatt aaataagtta t
//
