LOCUS       sequence-468957-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For production of Extracellular Vesicles (EVs), with Epithelial 
            Growth Factor Receptor on their surface, Strep-tag II labeled on its
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo 
            L, Brilot A, Marcotte EM, Ke Z, Leahy DJ
  TITLE     Structure and organization of full-length Epidermal Growth Factor 
            Receptor in extracellular vesicles by cryo-electron tomography.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 
            10.1101/2024.11.25.625301.
  PUBMED    39651119
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 
            10.1101/2024.11.25.625301."
COMMENT     Sequence Label: pStreptagII 3C EGFR EABR
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..54)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    43..267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      313..741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(400..419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     610..631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(750..770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        755..1084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      935..1070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     997..1016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1151..1945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1205..1224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1815..1834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2119..2240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2156..2175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2210..2229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2289..2305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2289..2305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2302..2324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2313..2329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2337..2343,2344..2361,2362..2367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2382..2403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2520..2537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2691..3279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2771..2790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3450..4241,4242..4310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4311..4415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4490..4509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4681..5060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5061..5264
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5215..5235
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5309..5328
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5309..5327
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5471..5494
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5507..5530
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
       61 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      121 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      181 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      241 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
      301 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      721 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
      781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      841 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      901 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      961 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1021 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1081 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     1141 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     1201 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     1261 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     1321 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     1381 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     1441 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     1501 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     1561 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     1621 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     1681 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     1741 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     1801 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     1861 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     1921 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     1981 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     2041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     2101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     2281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4441 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     4501 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     4561 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     4621 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     4681 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4741 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4801 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4861 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4921 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4981 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     5041 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     5101 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5161 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     5221 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     5281 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
     5341 gctagcgttt aaacttaagc ttggtaccgg atcctctaga gccaccatgc gaccctccgg
     5401 gacggccggg gcagcgctcc tggcgctgct ggctgcgctc tgcccggcga gtcgggctgg
     5461 cggaggcagc tggagccacc cgcagttcga aaagggagga ggctcccttg aagtcctctt
     5521 tcagggaccc ggcgggggct ccctggagga aaagaaagtt tgccaaggca cgagtaacaa
     5581 gctcacgcag ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa
     5641 ctgtgaggtg gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc
     5701 cttcttaaag accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga
     5761 gcgaattcct ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta
     5821 tgccttagca gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat
     5881 gagaaattta caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg
     5941 caacgtggag agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc
     6001 gatggacttc cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg
     6061 gagctgctgg ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca
     6121 gcagtgctcc gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc
     6181 tgcaggctgc acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga
     6241 agccacgtgc aaggacacct gccccccact catgctctac aaccccacca cgtaccagat
     6301 ggatgtgaac cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg
     6361 taattatgtg gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga
     6421 gatggaggaa gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg
     6481 taacggaata ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa
     6541 acacttcaaa aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag
     6601 gggtgactcc ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac
     6661 cgtaaaggaa atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct
     6721 ccatgccttt gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc
     6781 tcttgcagtc gtcagcctga acataacatc cttgggatta cgctccctca aggagataag
     6841 tgatggagat gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg
     6901 gaaaaaactg tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa
     6961 cagctgcaag gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg
     7021 cccggagccc agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga
     7081 caagtgcaac cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca
     7141 gtgccaccca gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga
     7201 caactgtatc cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc
     7261 aggagtcatg ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg
     7321 ccacctgtgc catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc
     7381 aacgaatggg cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct
     7441 gctggtggtg gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg
     7501 cacgctgcgg aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga
     7561 agctcccaac caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt
     7621 gctgggctcc ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa
     7681 agttaaaatt cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa
     7741 ggaaatcctc gatgaagcct acgtgatggc cagcgtggac aacccccacg tgtgccgcct
     7801 gctgggcatc tgcctcacct ccaccgtgca actcatcacg cagctcatgc ccttcggctg
     7861 cctcctggac tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg
     7921 gtgtgtgcag atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga
     7981 cctggcagcc aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg
     8041 gctggccaaa ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc
     8101 tatcaagtgg atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt
     8161 ctggagctac ggggtgaccg tttgggagtt gatgaccttt ggatccaagc catatgacgg
     8221 aatccctgcc agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc
     8281 catatgtacc atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag
     8341 tcgcccaaag ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg
     8401 ctaccttgtc attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt
     8461 ctaccgtgcc ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct
     8521 catcccacag cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc
     8581 tctgagtgca accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag
     8641 ctgtcccatc aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt
     8701 gactgaggac agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt
     8761 tcccaaaagg cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc
     8821 cgcgcccagc agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga
     8881 gtatctcaac actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg
     8941 ggcccagaaa ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt
     9001 tcccaaggaa gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata
     9061 cctaagggtc gcgccacaaa gcagtgaatt tattggagca agcggccgcg ctttgcctgg
     9121 caatcccgat cacagggaaa tgggggaaac tctcccagaa gaggttggag aatatcggca
     9181 gccatctggt ggcagtgtac ctgtgtctcc gggtccgccc agtggtcttg aacccactag
     9241 ctcctcacct tacggcggcg gctcctttaa cagctcaatc aacaacatcc acgaaatgga
     9301 gattcagctg aaggatgcac tcgaaaagaa tcaacaatgg ctcgtttacg accaacaacg
     9361 cgaggtgtac gtgaaaggat tgcttgcaaa aattttcgaa ctggaaaaga aaacagagac
     9421 tgcagcacat agtcttcctt gac
//
