LOCUS       sequence-469002-        5848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Standard for type I interferon signature (6 genes).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Tesser A, Bocca P, Ulivi M, Pin A, Pastorino C, Cangelosi D, Santori
            E, Drago E, Caorsi R, Candotti F, Gattorno M, Tommasini A, Volpi S
  TITLE     Type I Interferon Signature: a Quantitative Standardized Method for 
            Clinical Application.
  JOURNAL   Clin Exp Immunol. 2025 Mar 24:uxaf018. doi: 10.1093/cei/uxaf018.
  PUBMED    40127048
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Exp 
            Immunol. 2025 Mar 24:uxaf018. doi: 10.1093/cei/uxaf018."
COMMENT     Sequence Label: 6-ISG-IFN
FEATURES             Location/Qualifiers
     source          1..5848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3051..3070)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3052..3070)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3077..3094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3077..3093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3086..3108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3234..3662
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3321..3340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3531..3552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4006..4800
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4060..4079)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4670..4689
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4818..4886,4887..5678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5036..5055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg gtaccatgat
     1021 taccgcagca aaccgcttgg gattatattc ggcgtttcgg gcacgaagtg caatggtctt
     1081 taggtcaagt ttacaaccaa gattcactgt ggatacaata ttttgcagct gcggtacaat
     1141 cccagaactc tccgaagctg gcgtggcagg agtgatgggg gtcatgggag tcatgggggg
     1201 gggatacagt ggagtggggt accgagctcg gatccactag taacggccgc cagtgtgctg
     1261 gaattcgccc tttgcatgct gcgctggtcc aaccgcatgc ggccgcattc tcaggaactc
     1321 tccttctttg ggtctggctg aagttgagga tctcttactc tctaggccac ggaattaacc
     1381 cgagcaggca tggaggcctc tgctctcacc tcatcagtgg ccaaagtggt cagggtggcc
     1441 tctggctctg ccgtagtttt gcccctggcc aggattgcta cagttgtgat tgggtggggt
     1501 ctgatatcac cagcataacc gagcggtata ggatatattc tgttaaagat ggaaaaaatg
     1561 gaaaatctct gccatttatg ttgtgtgaca ctatggggct agatggggca gaaggagcag
     1621 gactgtgcat ggatgacatt ccccacatct taaaaggttg tatgccagac agatatcagt
     1681 ttaattcccg taaaccaatc cacaagacag aatagccaga tctcagagga gcctggctaa
     1741 gcaaaaccct gcagaacggc tgcctaattt acagcaacca tgagtacaaa tggtgatgat
     1801 catcaggtca aggatagtct ggagcaattg agatgtcact ttacatggga gttatccatt
     1861 gatgacgatg aaatgcctga ttgaggcagc gaactcatct ttgccagtac aggagcttgt
     1921 gccgtggccc acagcccaca gcccacagcc atgggctggg acctgacggt gaagatgctg
     1981 gcgggcaacg aattccaggt gtccctgagc agctccatgt cggtgtcaga gctgaaggcg
     2041 cagatcaccc agaagatcgg cgtgcacgcc ttctaaaccc tgtccgctgg aaagtgttcc
     2101 agtgcctctt aattgagggt gagaattgtg gagaagatgc tctaagagaa gcagaaagat
     2161 ttgttattgg tgatgaagaa tttgaaagat tcttacaaat gcggcttctg tttccacaca
     2221 gccaaaacat cctttgtgct gccccttgag gaagcaaaga gaggattgct tttgcttaag
     2281 gaagctggta tggagaagat caacttttca ggtggagagc catttccagt catgctccgt
     2341 gtgctctacc ctcccaagac gcccaccatg atggtcttcg tggagcctga gggtggcctc
     2401 cggggcatcc tctgccctgc aagtcctcta tgcccctcag gacgctgtcc tgtcctcctt
     2461 ccgggactcc agggccagat ccatggctgt gatacagtgc actgtggaca gtgagccacc
     2521 tgctgtgtga tgaaggagat gggaggccat cacattgtag ccctctgtgt gctcaagggg
     2581 ggctataaat tctttgctga cctgctggat tacatcaaag cactgaatag aaatgacctt
     2641 gatttatttt gcatacctaa tcattatgct gaggatttgg aaagggtgtt tattcctcat
     2701 ggactaatta tggacaggac tgaacgtctt gctcgttaac gaggatccat tggagggcaa
     2761 gtctggtgcc agcagccgcg gtaattccag ctccaatagc gtatattaaa gttgctgcag
     2821 ttaaaaagct cgtagttgga tcttgggagc gggcgggcgg tccgccgcga ggcgagcaaa
     2881 cagagtgagc ccttcttcaa ggccacccca gaggagaagc tcaagctgga ggacttcttt
     2941 gcccgcaact cctcgaggcc tcacgtggct cagcttcacg caagggcgaa ttctgcagat
     3001 atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg ccctatagtg
     3061 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3121 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3181 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     3241 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3301 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3361 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3421 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3481 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3541 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3601 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3661 ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
     3721 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     3781 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
     3841 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     3901 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     3961 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4021 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4081 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4141 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4201 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4261 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatcc
     4321 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4381 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4441 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4501 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4561 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4621 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4681 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4741 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5821 accccgtaga aaagatcaaa ggatcttc
//
