LOCUS       sequence-469005-        7966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red light-inducible Cre-N; Cre-N270-PhyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: CT619
FEATURES             Location/Qualifiers
     source          1..7966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      916..1504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1405..1424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1658..1675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1743..1877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1767..1786)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1821..1840
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1897..2031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2116..2137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2152..2157,2158..2175,2176..2182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2190..2206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2195..2217
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2214..2230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2214..2230
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2573..2592
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2591..2646
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2692..2711
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2951..3175
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3185..3205)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6013..6032)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6040..7056
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6094..6116)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7057..7334)
                     /label=chicken beta-actin promoter
     enhancer        7336..7715
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7746..7850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7851..7919,7920..7966,1..745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
       61 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1681 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1741 cggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1801 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1861 gctgcaataa acaagttagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     1921 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     1981 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg actatggttg
     2041 ctgactaatt gagatgcgga tccgctggca cgacaggttt cccgactgga aagcgggcag
     2101 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2161 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2221 cagctatgac catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa
     2281 gagggacagc tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat
     2341 gtaaggaaaa ttttagggat gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa
     2401 tctaacctca agtcaaggct tttctatgga ataaggaatg gacagcaggg ggctgtttca
     2461 tatactgatg acctctttat agccaacctt tgttcatggc agccagcata tgggcatatg
     2521 ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg
     2581 tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc
     2641 tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag
     2701 agggaaaaag atctcagtgg tatttgtgag ccagggcatt ggccacacca gccaccacct
     2761 tctgataggc agcctgcacc tgaggagtgc gctagcggtg gaagggctcg gagttgtggt
     2821 aatctatgta tcctgggcgg ccgcgcttgg attctgcgtt tgtttccgtc tacgaactcc
     2881 cagcggtaca cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc
     2941 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     3001 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     3061 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     3121 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacaggaatt
     3181 ctcacacctt ccgctttttc ttgggttgga ttccatcaat cttgagatcg ttgagcttgc
     3241 tatagatcac ctttgtgttc acgtcggtgg tctcgctgtc cttaaaggcg ttacgtaaga
     3301 tgagctgtaa agagtggatg gcatccattt cgtagtcctt ccaaggtaag cttctggtct
     3361 tcaccacctc cagaaaagcc ttgaaggagg atctggggtg catccttctg gcatcgtccc
     3421 tatcatcggg gtcgtgtttg gcgccgcccc atctcacttc gccggcggta tgggaacgaa
     3481 accagaaaat catatctttg ctgctgatac gaacggcagc cattccgcag acgctgtcgc
     3541 ctaaagataa agccctagga aagccagcgt cgtgtaaaga atcggtgctt aaaccggtgg
     3601 aatccatgtg gtactcacac agccaagaag cgatttcttg taaatggaac tcgctagggg
     3661 tggtgcccag cttccagatc ttgtccttgt ataataaggc ggcgccgtcg cacttgacta
     3721 aatccataat attgggggac tggctcacga tgccgagggg agcgtccctc atgagcatgt
     3781 cgcagaggag tgtctgggta cgtaaaatgt tcttctccac catctggttg tccagctcga
     3841 cctccttgtt gacgtggatg gcaaacactt gggctaaaaa ttcacaagca taacgtaagg
     3901 gaaaagggac aaatctgggg gtggtgttgt ggcagaccac taaaccccac agcctctttc
     3961 tcttctgagg ctgtgtggtg gcatcgggag catcgccctc tccatcctcc tcattcacca
     4021 ccacggccat cactaaagaa gcgatgctgt ccatattggc catgtattgc agatgacaag
     4081 agtggggagc tcttaaagtg ctgccgcaga gggttaaatc gaagctcagc ttctcatctt
     4141 gtaaaaccct agcgtgcttg gcgttgcagt ccacgatcat tctcaccttg ttcttcataa
     4201 ataaaaatct ggcagcttgg gggatgtctg tggcgggata gtgtaaacct aaatagggtt
     4261 ctaaaccggg cttggtcact tcgctcacca cctcgccgtg gtcgtcctcg tggaacttgt
     4321 aggccatgac acgatcgtaa ccagtcagct cgaagacttc ttgcaccatg gtgtcgcaca
     4381 gcctctccat gcttccgctg ggtaaagact ggagtctggt gatggccttg gcggccagct
     4441 tatagctctg taaagctccg gcggctgtca tgggcacttc atagggctta acgggttcga
     4501 agtcgatgat gatggatccg gtcaccctat ggataatggc gtagaagggc ttggcggatg
     4561 tacgacaatg gacgaggata gggttcagta aagacacgtc gccgaagccg agagccttct
     4621 gtaaagcgga agcgctggga gcggtaaata aagatctgat atcggtgccg atgcccagaa
     4681 cggggtgctc tcccacgcta ggcacggcgt gggaagccat tgtgagcagt tcggaggcgt
     4741 tctcggagta ggcgatgacc ttgaaggtct tttcgtctaa agccagtaaa catccaaagg
     4801 gctggatcag cttgcccttc tggatatgat ggaggtaggt tgtggtgacc ttgtcgctcc
     4861 taggaggctg gttctcgacc acgggaccag tcaccctcac gctggtggag taatcaaagg
     4921 aggagccgct ctcctcgaaa tcggcgtgca gtttggcgtc cacggtggtc tgagcgatga
     4981 tacgagcgga gtgacgagat ctacgagatc cctcgctgga ctgtgtggga cgagatccgc
     5041 tcatcttctt ctcggtaccg ctgccaccac ttgatccgga gccagaccct ccggagcggt
     5101 gggtggcctc aaagatccct tccagggccc gggtggacag ttgggaggtg gcagaagggg
     5161 cagccacacc attctttctg acccggcaga acaggtagtt gttggggtca tcagccacac
     5221 cagacacaga gatccatctc tccaccagct tggtaacccc cagggacagg gccttctcca
     5281 caccagctgt ggacaccagg gtcttggtcc tgccaatgtg gatcagcatt ctcccaccat
     5341 cggtgcggga gatgtccttc actctgattc tggcaatttc ggcaatgcgc agcagggtgt
     5401 tgtaggcaat gcccaggaag gccaggttcc tgatgtcctg gcatctgtca gagttctcca
     5461 tcagggatct gacttggtca aagtcagtgc gttcaaaggc cagggcctgc ttggctctct
     5521 ccccagcatc cacattctcc tttctgattc tcctcatcac cagggacaca gcattggagt
     5581 cagaagggcg aggcaggcca gatctcctgt gcagcatgtt gagctggccc aggtgctgtt
     5641 ggatggtctt cacagccagg cctctggctt gcaggtacag gaggtagtcc ctcacatcct
     5701 caggttcagc agggaaccat ttcctgttgt tcagcttgca ccaggcagcc caggatctgc
     5761 acacagacag gagcatcttc caggtgtgtt cagagaaggc ctgcctgtcc ctgaacatgt
     5821 ccatcaggtt cttcctgact tcatcagagg tggcatccac agggagggca ggcaggtttt
     5881 ggtgcacagt cagcaggttg gacatggtgg cggcacgcgt gcgagacgag acgagacagc
     5941 ctgagaatgg atgcgagtaa tgggcgcgcc ttcgctcgaa ttaatcaatt ctttgccaaa
     6001 atgatgagac agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc
     6061 atgaacatgg ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc
     6121 cctgccccgt cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg
     6181 gagaagggga cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc
     6241 ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt
     6301 cggctccgca cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa
     6361 aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg
     6421 cccctccccc gagccctccc cggcccgagg cggccccgcc ccgcccggca cccccacctg
     6481 ccgccacccc ccgcccggca cggcgagccc cgcgccacgc cccgtacgga gccccgcacc
     6541 cgaagccggg ccgtgctcag caactcgggg aggggggtgc agggggggtt gcagcccgac
     6601 cgacgcgccc acaccccctg ctcacccccc cacgcacaca ccccgcacgc agcctttgtt
     6661 cccctcgcag ccccccccgc accgcggggc accgcccccg gccgcgctcc cctcgcgcac
     6721 actgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc
     6781 ggagccgcac gcggcgctcc ccacgcacac acacacgcac gcaccccccg agccgctccc
     6841 cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac
     6901 aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct
     6961 cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga
     7021 gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt
     7081 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
     7141 attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc
     7201 gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     7261 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     7321 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     7381 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     7441 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     7501 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     7561 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     7621 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     7681 tgattactat taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa
     7741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttggg
//
